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Separation and determination of enkephalin-related peptides by capillary electrophoresis with amperometric detection.
J Sep Sci. 2008 Feb; 31(3):574-8.JS

Abstract

A simple, reliable, and reproducible method for separation and determination of five enkephalin-related peptides based on CE with amperometric detection (AD) is described in this paper. A potential of 1.0 V was applied to the carbon disk electrode, which was used as a working electrode in this system. At 15 kV of applied voltage, the five compounds were separated within 18 min in a 20 mmol/L phosphate buffer solution (PBS, pH 7.6) including 2.5% methanol v/v. LOD for five enkephalins were ranged from 6.31 to 54.3 nmol/L. The method was applied successfully to determine the five compounds added in the human cerebrospinal fluid (CSF) and the recoveries were in the range of 95.8-98.2%.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang W
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fujian, China.
Yao S
No affiliation info available
Qiu B
No affiliation info available
Chen G
No affiliation info available

MeSH

CarbonConductometryElectrodesElectrophoresis, CapillaryEnkephalinsHydrogen-Ion ConcentrationPeptidesReproducibility of ResultsSensitivity and SpecificityTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18219656

Citation

Wang, Wei, et al. "Separation and Determination of Enkephalin-related Peptides By Capillary Electrophoresis With Amperometric Detection." Journal of Separation Science, vol. 31, no. 3, 2008, pp. 574-8.
Wang W, Yao S, Qiu B, et al. Separation and determination of enkephalin-related peptides by capillary electrophoresis with amperometric detection. J Sep Sci. 2008;31(3):574-8.
Wang, W., Yao, S., Qiu, B., & Chen, G. (2008). Separation and determination of enkephalin-related peptides by capillary electrophoresis with amperometric detection. Journal of Separation Science, 31(3), 574-8. https://doi.org/10.1002/jssc.200700538
Wang W, et al. Separation and Determination of Enkephalin-related Peptides By Capillary Electrophoresis With Amperometric Detection. J Sep Sci. 2008;31(3):574-8. PubMed PMID: 18219656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of enkephalin-related peptides by capillary electrophoresis with amperometric detection. AU - Wang,Wei, AU - Yao,Sheyue, AU - Qiu,Bin, AU - Chen,Guonan, PY - 2008/1/26/pubmed PY - 2008/4/26/medline PY - 2008/1/26/entrez SP - 574 EP - 8 JF - Journal of separation science JO - J Sep Sci VL - 31 IS - 3 N2 - A simple, reliable, and reproducible method for separation and determination of five enkephalin-related peptides based on CE with amperometric detection (AD) is described in this paper. A potential of 1.0 V was applied to the carbon disk electrode, which was used as a working electrode in this system. At 15 kV of applied voltage, the five compounds were separated within 18 min in a 20 mmol/L phosphate buffer solution (PBS, pH 7.6) including 2.5% methanol v/v. LOD for five enkephalins were ranged from 6.31 to 54.3 nmol/L. The method was applied successfully to determine the five compounds added in the human cerebrospinal fluid (CSF) and the recoveries were in the range of 95.8-98.2%. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/18219656/Separation_and_determination_of_enkephalin_related_peptides_by_capillary_electrophoresis_with_amperometric_detection_ DB - PRIME DP - Unbound Medicine ER -