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Optimization of the preparation of glass-coated, dye-tagged metal nanoparticles as SERS substrates.
Langmuir. 2008 Mar 04; 24(5):2178-85.L

Abstract

Dye-tagged metal nanoparticles are of significant interest in SERS-based sensitive detection applications. Coating these particles in glass results in an inert spectral tag that can be used in applications such as flow cytometry with significant multiplexing potential. Maximizing the SERS signal obtainable from these particles requires care in partitioning available nanoparticle surface area (binding sites) between the SERS dyes and the functionalized silanes necessary for anchoring the glass coating. In this article, we use the metal-mediated fluorescence quenching of SERS dyes to measure surface areas occupied by both dyes and silanes and thus examine SERS intensities as a function of both dye and silane loading. Notably, we find that increased surface occupation by silane increases the aggregative power of added dye but that decreasing the silane coverage allows a greater surface concentration of dye. Both effects increase the SERS intensity, but obtaining the optimum SERS intensity will require balancing aggregation against surface dye concentration.

Authors+Show Affiliations

Chemical Sciences and Engineering, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA. leif@lanl.govNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18220434

Citation

Brown, Leif O., and Stephen K. Doorn. "Optimization of the Preparation of Glass-coated, Dye-tagged Metal Nanoparticles as SERS Substrates." Langmuir : the ACS Journal of Surfaces and Colloids, vol. 24, no. 5, 2008, pp. 2178-85.
Brown LO, Doorn SK. Optimization of the preparation of glass-coated, dye-tagged metal nanoparticles as SERS substrates. Langmuir. 2008;24(5):2178-85.
Brown, L. O., & Doorn, S. K. (2008). Optimization of the preparation of glass-coated, dye-tagged metal nanoparticles as SERS substrates. Langmuir : the ACS Journal of Surfaces and Colloids, 24(5), 2178-85. https://doi.org/10.1021/la703218f
Brown LO, Doorn SK. Optimization of the Preparation of Glass-coated, Dye-tagged Metal Nanoparticles as SERS Substrates. Langmuir. 2008 Mar 4;24(5):2178-85. PubMed PMID: 18220434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of the preparation of glass-coated, dye-tagged metal nanoparticles as SERS substrates. AU - Brown,Leif O, AU - Doorn,Stephen K, Y1 - 2008/01/26/ PY - 2008/1/29/pubmed PY - 2008/5/28/medline PY - 2008/1/29/entrez SP - 2178 EP - 85 JF - Langmuir : the ACS journal of surfaces and colloids JO - Langmuir VL - 24 IS - 5 N2 - Dye-tagged metal nanoparticles are of significant interest in SERS-based sensitive detection applications. Coating these particles in glass results in an inert spectral tag that can be used in applications such as flow cytometry with significant multiplexing potential. Maximizing the SERS signal obtainable from these particles requires care in partitioning available nanoparticle surface area (binding sites) between the SERS dyes and the functionalized silanes necessary for anchoring the glass coating. In this article, we use the metal-mediated fluorescence quenching of SERS dyes to measure surface areas occupied by both dyes and silanes and thus examine SERS intensities as a function of both dye and silane loading. Notably, we find that increased surface occupation by silane increases the aggregative power of added dye but that decreasing the silane coverage allows a greater surface concentration of dye. Both effects increase the SERS intensity, but obtaining the optimum SERS intensity will require balancing aggregation against surface dye concentration. SN - 0743-7463 UR - https://www.unboundmedicine.com/medline/citation/18220434/Optimization_of_the_preparation_of_glass_coated_dye_tagged_metal_nanoparticles_as_SERS_substrates_ L2 - https://doi.org/10.1021/la703218f DB - PRIME DP - Unbound Medicine ER -