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Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas.
Clin Endocrinol (Oxf). 2008 Sep; 69(3):400-6.CE

Abstract

OBJECTIVES

The pancreatic ducts, endocrine islets and exocrine acini are three functionally related components. From birth to adulthood, the islets and ducts are regarded as independent entities. The objective of this study is to investigate the topographical associations between the islet endocrine cells and duct epithelial cells in the adult human pancreas.

MATERIALS AND METHODS

Panels of immunomarkers for the exocrine acinar cells (amylase), duct cells [cytokeratin 19 (CK19)], endocrine cells (chromogranin A, neurone specific enolase, synaptophysin) and islet hormones (glucagon, insulin, somatostatin, pancreatic polypeptide) were applied to sequential pancreatic tissue sections obtained from autopsy specimens of 10-nondiabetic human adults. Double immunofluorescent staining with CK19 and islet hormones was performed to confirm the islet to duct interrelationship.

RESULTS

Sequential sectioning and immunostaining showed that 45% of the 172 islets examined appeared as single endocrine cell units or small clusters of < 10 endocrine cells on at least one plane of section. A topographical association was found between the islet endocrine cells and duct epithelial cells. Topographical associations with CK 19-stained duct cells occurred in 10.9% of the islet insulin-containing beta-cells and in 8.9% of the islet glucagon-producing alpha-cells. The frequency of topographical associations increased toward the more distally located duct systems. The CK19-stained duct cells and amylase-labelled acinar cells were less frequently in association with other islet hormone-producing cells.

CONCLUSIONS

Topographical associations between islet endocrine cells and pancreatic duct cells are frequent in adult human pancreas. The islet-duct association suggests possible functional interactions between the two interrelated pancreatic compartments.

Authors+Show Affiliations

Department of Medicine and Therapeutics, the Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong SAR, China. zhaohailu@cuhk.edu.hkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18221396

Citation

Zhao, Hai-Lu, et al. "Topographical Associations Between Islet Endocrine Cells and Duct Epithelial Cells in the Adult Human Pancreas." Clinical Endocrinology, vol. 69, no. 3, 2008, pp. 400-6.
Zhao HL, Sui Y, Guan J, et al. Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas. Clin Endocrinol (Oxf). 2008;69(3):400-6.
Zhao, H. L., Sui, Y., Guan, J., Lai, F. M., Gu, X. M., He, L., Zhu, X., Rowlands, D. K., Xu, G., Tong, P. C., & Chan, J. C. (2008). Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas. Clinical Endocrinology, 69(3), 400-6. https://doi.org/10.1111/j.1365-2265.2008.03190.x
Zhao HL, et al. Topographical Associations Between Islet Endocrine Cells and Duct Epithelial Cells in the Adult Human Pancreas. Clin Endocrinol (Oxf). 2008;69(3):400-6. PubMed PMID: 18221396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Topographical associations between islet endocrine cells and duct epithelial cells in the adult human pancreas. AU - Zhao,Hai-Lu, AU - Sui,Yi, AU - Guan,Jing, AU - Lai,Fernand M M, AU - Gu,Xue-Mei, AU - He,Lan, AU - Zhu,Xun, AU - Rowlands,Dewi K, AU - Xu,Gang, AU - Tong,Peter C Y, AU - Chan,Juliana C N, Y1 - 2008/01/21/ PY - 2008/1/29/pubmed PY - 2009/7/25/medline PY - 2008/1/29/entrez SP - 400 EP - 6 JF - Clinical endocrinology JO - Clin Endocrinol (Oxf) VL - 69 IS - 3 N2 - OBJECTIVES: The pancreatic ducts, endocrine islets and exocrine acini are three functionally related components. From birth to adulthood, the islets and ducts are regarded as independent entities. The objective of this study is to investigate the topographical associations between the islet endocrine cells and duct epithelial cells in the adult human pancreas. MATERIALS AND METHODS: Panels of immunomarkers for the exocrine acinar cells (amylase), duct cells [cytokeratin 19 (CK19)], endocrine cells (chromogranin A, neurone specific enolase, synaptophysin) and islet hormones (glucagon, insulin, somatostatin, pancreatic polypeptide) were applied to sequential pancreatic tissue sections obtained from autopsy specimens of 10-nondiabetic human adults. Double immunofluorescent staining with CK19 and islet hormones was performed to confirm the islet to duct interrelationship. RESULTS: Sequential sectioning and immunostaining showed that 45% of the 172 islets examined appeared as single endocrine cell units or small clusters of < 10 endocrine cells on at least one plane of section. A topographical association was found between the islet endocrine cells and duct epithelial cells. Topographical associations with CK 19-stained duct cells occurred in 10.9% of the islet insulin-containing beta-cells and in 8.9% of the islet glucagon-producing alpha-cells. The frequency of topographical associations increased toward the more distally located duct systems. The CK19-stained duct cells and amylase-labelled acinar cells were less frequently in association with other islet hormone-producing cells. CONCLUSIONS: Topographical associations between islet endocrine cells and pancreatic duct cells are frequent in adult human pancreas. The islet-duct association suggests possible functional interactions between the two interrelated pancreatic compartments. SN - 1365-2265 UR - https://www.unboundmedicine.com/medline/citation/18221396/Topographical_associations_between_islet_endocrine_cells_and_duct_epithelial_cells_in_the_adult_human_pancreas_ L2 - https://doi.org/10.1111/j.1365-2265.2008.03190.x DB - PRIME DP - Unbound Medicine ER -