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Determination of ofloxacin enantiomers in sewage using two-step solid-phase extraction and liquid chromatography with fluorescence detection.
J Chromatogr A. 2008 Feb 22; 1182(1):77-84.JC

Abstract

A robust analytical method has been developed and validated for the trace analysis of ofloxacin enantiomers in sewage using two-step solid-phase extraction purification and liquid chromatography with fluorescence detection (LC-FL). Ofloxacin enantiomers were separated on an Aglient TC-C-18 column using MeOH-water containing 4mmol/L CuSO4 and 5mmol/L l-isoleucine as mobile phase. The ofloxacin enantiomers were first extracted by a weak cation-exchange resin (WCX) and eluted with acidified MeOH (0.5% formic acid), then further purified by mixed mode of anion-exchange resin (MAX), resulting in ofloxacin recoveries generally above 95%. The limit of quantification was 0.08microg/L for each enantiomer. No significant matrix effect was found during the analytical procedure and standard solution calibration curves could be used for quantification. Total concentrations of both enantiomers in real sewage samples based on LC-FL method were consistent with those obtained upon liquid chromatography using tandem mass spectrometry (LC-MS/MS) method.

Authors+Show Affiliations

Beijing Center for Disease Control and Prevention, Beijing 100013, China. shaobingch@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18221747

Citation

Shao, Bing, et al. "Determination of Ofloxacin Enantiomers in Sewage Using Two-step Solid-phase Extraction and Liquid Chromatography With Fluorescence Detection." Journal of Chromatography. A, vol. 1182, no. 1, 2008, pp. 77-84.
Shao B, Sun X, Zhang J, et al. Determination of ofloxacin enantiomers in sewage using two-step solid-phase extraction and liquid chromatography with fluorescence detection. J Chromatogr A. 2008;1182(1):77-84.
Shao, B., Sun, X., Zhang, J., Hu, J., Dong, H., & Yang, Y. (2008). Determination of ofloxacin enantiomers in sewage using two-step solid-phase extraction and liquid chromatography with fluorescence detection. Journal of Chromatography. A, 1182(1), 77-84. https://doi.org/10.1016/j.chroma.2007.12.073
Shao B, et al. Determination of Ofloxacin Enantiomers in Sewage Using Two-step Solid-phase Extraction and Liquid Chromatography With Fluorescence Detection. J Chromatogr A. 2008 Feb 22;1182(1):77-84. PubMed PMID: 18221747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of ofloxacin enantiomers in sewage using two-step solid-phase extraction and liquid chromatography with fluorescence detection. AU - Shao,Bing, AU - Sun,Xiaojie, AU - Zhang,Jing, AU - Hu,Jianying, AU - Dong,Huiru, AU - Yang,Yi, Y1 - 2008/01/06/ PY - 2007/07/28/received PY - 2007/12/19/revised PY - 2007/12/21/accepted PY - 2008/1/29/pubmed PY - 2008/5/20/medline PY - 2008/1/29/entrez SP - 77 EP - 84 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1182 IS - 1 N2 - A robust analytical method has been developed and validated for the trace analysis of ofloxacin enantiomers in sewage using two-step solid-phase extraction purification and liquid chromatography with fluorescence detection (LC-FL). Ofloxacin enantiomers were separated on an Aglient TC-C-18 column using MeOH-water containing 4mmol/L CuSO4 and 5mmol/L l-isoleucine as mobile phase. The ofloxacin enantiomers were first extracted by a weak cation-exchange resin (WCX) and eluted with acidified MeOH (0.5% formic acid), then further purified by mixed mode of anion-exchange resin (MAX), resulting in ofloxacin recoveries generally above 95%. The limit of quantification was 0.08microg/L for each enantiomer. No significant matrix effect was found during the analytical procedure and standard solution calibration curves could be used for quantification. Total concentrations of both enantiomers in real sewage samples based on LC-FL method were consistent with those obtained upon liquid chromatography using tandem mass spectrometry (LC-MS/MS) method. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18221747/Determination_of_ofloxacin_enantiomers_in_sewage_using_two_step_solid_phase_extraction_and_liquid_chromatography_with_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00013-7 DB - PRIME DP - Unbound Medicine ER -