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Analysis of diuretic doping agents by HPLC screening and GC-MSD confirmation.
J Pharm Biomed Anal. 1991; 9(10-12):1069-76.JP

Abstract

The simultaneous analysis of 14 diuretics in human urine (belonging to five different pharmacological groups) by reversed-phase high-performance liquid chromatography (HPLC) with a diode-array detector was developed by using a gradient elution with acetonitrile and phosphate buffer containing propylamine hydrochloride on a Bondclone-ODS (10 microns) column. The method was applied to the screening test of several diuretics abused by athletes. The confirmation analysis was performed by gas chromatography-mass spectrometry with ion-selective detection (GC-MSD). The characteristic mass fragment ions obtained by electron-impact (EI) ionization (70 eV) provided sufficient identification of these diuretic agents. During the 1990 weight-lifting contest of Asia in Kao-Hsiung, Republic of China, one urine sample was found positive for the diuretic hydrochlorothiazide.

Authors+Show Affiliations

National Laboratory of Foods and Drugs, Department of Health, Taipei, Taiwan, Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1822175

Citation

Tsai, F Y., et al. "Analysis of Diuretic Doping Agents By HPLC Screening and GC-MSD Confirmation." Journal of Pharmaceutical and Biomedical Analysis, vol. 9, no. 10-12, 1991, pp. 1069-76.
Tsai FY, Lui LF, Chang B. Analysis of diuretic doping agents by HPLC screening and GC-MSD confirmation. J Pharm Biomed Anal. 1991;9(10-12):1069-76.
Tsai, F. Y., Lui, L. F., & Chang, B. (1991). Analysis of diuretic doping agents by HPLC screening and GC-MSD confirmation. Journal of Pharmaceutical and Biomedical Analysis, 9(10-12), 1069-76.
Tsai FY, Lui LF, Chang B. Analysis of Diuretic Doping Agents By HPLC Screening and GC-MSD Confirmation. J Pharm Biomed Anal. 1991;9(10-12):1069-76. PubMed PMID: 1822175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of diuretic doping agents by HPLC screening and GC-MSD confirmation. AU - Tsai,F Y, AU - Lui,L F, AU - Chang,B, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 1069 EP - 76 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 9 IS - 10-12 N2 - The simultaneous analysis of 14 diuretics in human urine (belonging to five different pharmacological groups) by reversed-phase high-performance liquid chromatography (HPLC) with a diode-array detector was developed by using a gradient elution with acetonitrile and phosphate buffer containing propylamine hydrochloride on a Bondclone-ODS (10 microns) column. The method was applied to the screening test of several diuretics abused by athletes. The confirmation analysis was performed by gas chromatography-mass spectrometry with ion-selective detection (GC-MSD). The characteristic mass fragment ions obtained by electron-impact (EI) ionization (70 eV) provided sufficient identification of these diuretic agents. During the 1990 weight-lifting contest of Asia in Kao-Hsiung, Republic of China, one urine sample was found positive for the diuretic hydrochlorothiazide. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/1822175/Analysis_of_diuretic_doping_agents_by_HPLC_screening_and_GC_MSD_confirmation_ DB - PRIME DP - Unbound Medicine ER -