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Analysis of the Arabidopsis O-acetylserine(thiol)lyase gene family demonstrates compartment-specific differences in the regulation of cysteine synthesis.
Plant Cell 2008; 20(1):168-85PC

Abstract

Cys synthesis in plants takes place in plastids, cytosol, and mitochondria. Why Cys synthesis is required in all compartments with autonomous protein biosynthesis and whether Cys is exchanged between them has remained enigmatic. This question was addressed using Arabidopsis thaliana T-DNA insertion lines deficient in the final step of Cys biosynthesis catalyzed by the enzyme O-acetylserine(thiol)lyase (OAS-TL). Null alleles of oastlA or oastlB alone showed that cytosolic OAS-TL A and plastid OAS-TL B were completely dispensable, although together they contributed 95% of total OAS-TL activity. An oastlAB double mutant, relying solely on mitochondrial OAS-TL C for Cys synthesis, showed 25% growth retardation. Although OAS-TL C alone was sufficient for full development, oastlC plants also showed retarded growth. Targeted affinity purification identified the major OAS-TL-like proteins. Two-dimensional gel electrophoresis and mass spectrometry showed no compensatory changes of OAS-TL isoforms in the four mutants. Steady state concentrations of Cys and glutathione and pulse-chase labeling with [35S]sulfate indicated strong perturbation of primary sulfur metabolism. These data demonstrate that Cys and also sulfide must be sufficiently exchangeable between cytosol and organelles. Despite partial redundancy, the mitochondria and not the plastids play the most important role for Cys synthesis in Arabidopsis.

Authors+Show Affiliations

Heidelberg Institute of Plant Sciences, University of Heidelberg, 69120 Heidelberg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18223034

Citation

Heeg, Corinna, et al. "Analysis of the Arabidopsis O-acetylserine(thiol)lyase Gene Family Demonstrates Compartment-specific Differences in the Regulation of Cysteine Synthesis." The Plant Cell, vol. 20, no. 1, 2008, pp. 168-85.
Heeg C, Kruse C, Jost R, et al. Analysis of the Arabidopsis O-acetylserine(thiol)lyase gene family demonstrates compartment-specific differences in the regulation of cysteine synthesis. Plant Cell. 2008;20(1):168-85.
Heeg, C., Kruse, C., Jost, R., Gutensohn, M., Ruppert, T., Wirtz, M., & Hell, R. (2008). Analysis of the Arabidopsis O-acetylserine(thiol)lyase gene family demonstrates compartment-specific differences in the regulation of cysteine synthesis. The Plant Cell, 20(1), pp. 168-85. doi:10.1105/tpc.107.056747.
Heeg C, et al. Analysis of the Arabidopsis O-acetylserine(thiol)lyase Gene Family Demonstrates Compartment-specific Differences in the Regulation of Cysteine Synthesis. Plant Cell. 2008;20(1):168-85. PubMed PMID: 18223034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of the Arabidopsis O-acetylserine(thiol)lyase gene family demonstrates compartment-specific differences in the regulation of cysteine synthesis. AU - Heeg,Corinna, AU - Kruse,Cordula, AU - Jost,Ricarda, AU - Gutensohn,Michael, AU - Ruppert,Thomas, AU - Wirtz,Markus, AU - Hell,Rüdiger, Y1 - 2008/01/25/ PY - 2008/1/29/pubmed PY - 2008/5/16/medline PY - 2008/1/29/entrez SP - 168 EP - 85 JF - The Plant cell JO - Plant Cell VL - 20 IS - 1 N2 - Cys synthesis in plants takes place in plastids, cytosol, and mitochondria. Why Cys synthesis is required in all compartments with autonomous protein biosynthesis and whether Cys is exchanged between them has remained enigmatic. This question was addressed using Arabidopsis thaliana T-DNA insertion lines deficient in the final step of Cys biosynthesis catalyzed by the enzyme O-acetylserine(thiol)lyase (OAS-TL). Null alleles of oastlA or oastlB alone showed that cytosolic OAS-TL A and plastid OAS-TL B were completely dispensable, although together they contributed 95% of total OAS-TL activity. An oastlAB double mutant, relying solely on mitochondrial OAS-TL C for Cys synthesis, showed 25% growth retardation. Although OAS-TL C alone was sufficient for full development, oastlC plants also showed retarded growth. Targeted affinity purification identified the major OAS-TL-like proteins. Two-dimensional gel electrophoresis and mass spectrometry showed no compensatory changes of OAS-TL isoforms in the four mutants. Steady state concentrations of Cys and glutathione and pulse-chase labeling with [35S]sulfate indicated strong perturbation of primary sulfur metabolism. These data demonstrate that Cys and also sulfide must be sufficiently exchangeable between cytosol and organelles. Despite partial redundancy, the mitochondria and not the plastids play the most important role for Cys synthesis in Arabidopsis. SN - 1040-4651 UR - https://www.unboundmedicine.com/medline/citation/18223034/Analysis_of_the_Arabidopsis_O_acetylserine_thiol_lyase_gene_family_demonstrates_compartment_specific_differences_in_the_regulation_of_cysteine_synthesis_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=18223034 DB - PRIME DP - Unbound Medicine ER -