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Artemisia princeps var orientalis induces apoptosis in human breast cancer MCF-7 cells.
Anticancer Res. 2007 Nov-Dec; 27(6B):3891-8.AR

Abstract

BACKGROUND

Dried leaves of Artemisia princeps var orientalis are used in the Eastern practice of moxibustion to improve general health. The ability of A. princeps smoke and water extracts to induce apoptosis was evaluated in human breast cancer MCF-7 cells in vitro.

MATERIALS AND METHODS

Tumor cells were cultured with a smoke or water extract (1.5-50% v/v) for 72 h, and cytotoxicity and apoptosis were determined by MTT and TUNEL assays, respectively. Activation of caspases, changes in membrane potential, and BCL-2 expression were determined by flow cytometry.

RESULTS

Both preparations inhibited the growth of breast cancer cells in a dose-dependent- manner. Induction of apoptosis was associated with activation of caspases 3, 8 and 9, depolarization of the mitochondrial membrane potential and down-regulation of BCL-2 expression. Furthermore, A. princeps smoke exerted synergistic cytotoxicity with doxorubicin.

CONCLUSION

The data suggest that A. princeps smoke and water soluble extracts induce apoptosis via the mitochondrial pathway and may represent a novel adjuvant for the treatment of breast cancer.

Authors+Show Affiliations

Division of Basic and Clinical Immunology, University of California, Irvine, CA, 92697, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18225547

Citation

Sarath, Vasiraju J., et al. "Artemisia Princeps Var Orientalis Induces Apoptosis in Human Breast Cancer MCF-7 Cells." Anticancer Research, vol. 27, no. 6B, 2007, pp. 3891-8.
Sarath VJ, So CS, Won YD, et al. Artemisia princeps var orientalis induces apoptosis in human breast cancer MCF-7 cells. Anticancer Res. 2007;27(6B):3891-8.
Sarath, V. J., So, C. S., Won, Y. D., & Gollapudi, S. (2007). Artemisia princeps var orientalis induces apoptosis in human breast cancer MCF-7 cells. Anticancer Research, 27(6B), 3891-8.
Sarath VJ, et al. Artemisia Princeps Var Orientalis Induces Apoptosis in Human Breast Cancer MCF-7 Cells. Anticancer Res. 2007 Nov-Dec;27(6B):3891-8. PubMed PMID: 18225547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Artemisia princeps var orientalis induces apoptosis in human breast cancer MCF-7 cells. AU - Sarath,Vasiraju J, AU - So,Chang-Sok, AU - Won,Young Doo, AU - Gollapudi,Sastry, PY - 2008/1/30/pubmed PY - 2008/2/13/medline PY - 2008/1/30/entrez SP - 3891 EP - 8 JF - Anticancer research JO - Anticancer Res VL - 27 IS - 6B N2 - BACKGROUND: Dried leaves of Artemisia princeps var orientalis are used in the Eastern practice of moxibustion to improve general health. The ability of A. princeps smoke and water extracts to induce apoptosis was evaluated in human breast cancer MCF-7 cells in vitro. MATERIALS AND METHODS: Tumor cells were cultured with a smoke or water extract (1.5-50% v/v) for 72 h, and cytotoxicity and apoptosis were determined by MTT and TUNEL assays, respectively. Activation of caspases, changes in membrane potential, and BCL-2 expression were determined by flow cytometry. RESULTS: Both preparations inhibited the growth of breast cancer cells in a dose-dependent- manner. Induction of apoptosis was associated with activation of caspases 3, 8 and 9, depolarization of the mitochondrial membrane potential and down-regulation of BCL-2 expression. Furthermore, A. princeps smoke exerted synergistic cytotoxicity with doxorubicin. CONCLUSION: The data suggest that A. princeps smoke and water soluble extracts induce apoptosis via the mitochondrial pathway and may represent a novel adjuvant for the treatment of breast cancer. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/18225547/Artemisia_princeps_var_orientalis_induces_apoptosis_in_human_breast_cancer_MCF_7_cells_ L2 - http://ar.iiarjournals.org/cgi/pmidlookup?view=long&pmid=18225547 DB - PRIME DP - Unbound Medicine ER -