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Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method.
Colloids Surf B Biointerfaces. 2008 Jun 01; 63(2):164-75.CS

Abstract

In this study, uniform-sized pH-sensitive quaternized chitosan microsphere was prepared by combining Shirasu porous glass (SPG) membrane emulsification technique and a novel thermal-gelation method. In this preparation process, the mixture of quaternized chitosan solution and alpha-beta-glycerophosphate (alpha-beta-GP) was used as water phase and dispersed in oil phase to form uniform W/O emulsion by SPG membrane emulsification technique. The droplets solidified into microspheres at 37 degrees C by thermal-gelation method. The whole process was simple and mild. The influence of process conditions on the property of prepared microspheres was investigated and the optimized preparation condition was obtained. As a result, the coefficient of variation (C.V.) of obtained microspheres diameters was below 15%. The obtained microsphere had porous structure and showed apparent pH-sensitivity. It dissolved rapidly in acid solution (pH 5) and kept stable in neutral solution (pH 7.4). The pH-sensitivity of microspheres also affected its drug release behavior. Bovine serum albumin (BSA) as a model drug was encapsulated in microspheres, and it was released rapidly in acid solution and slowly in neutral medium. The novel quaternized chitosan microspheres with pH-sensitivity can be used as drug delivery system in the biomedical field, such as tumor-targeted drug carrier.

Authors+Show Affiliations

State Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18226510

Citation

Wu, Jie, et al. "Preparation of Uniform-sized pH-sensitive Quaternized Chitosan Microsphere By Combining Membrane Emulsification Technique and Thermal-gelation Method." Colloids and Surfaces. B, Biointerfaces, vol. 63, no. 2, 2008, pp. 164-75.
Wu J, Wei W, Wang LY, et al. Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method. Colloids Surf B Biointerfaces. 2008;63(2):164-75.
Wu, J., Wei, W., Wang, L. Y., Su, Z. G., & Ma, G. H. (2008). Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method. Colloids and Surfaces. B, Biointerfaces, 63(2), 164-75. https://doi.org/10.1016/j.colsurfb.2007.11.021
Wu J, et al. Preparation of Uniform-sized pH-sensitive Quaternized Chitosan Microsphere By Combining Membrane Emulsification Technique and Thermal-gelation Method. Colloids Surf B Biointerfaces. 2008 Jun 1;63(2):164-75. PubMed PMID: 18226510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of uniform-sized pH-sensitive quaternized chitosan microsphere by combining membrane emulsification technique and thermal-gelation method. AU - Wu,Jie, AU - Wei,Wei, AU - Wang,Lian-Yan, AU - Su,Zhi-Guo, AU - Ma,Guang-Hui, Y1 - 2007/12/15/ PY - 2007/04/23/received PY - 2007/11/12/revised PY - 2007/11/27/accepted PY - 2008/1/30/pubmed PY - 2008/8/6/medline PY - 2008/1/30/entrez SP - 164 EP - 75 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 63 IS - 2 N2 - In this study, uniform-sized pH-sensitive quaternized chitosan microsphere was prepared by combining Shirasu porous glass (SPG) membrane emulsification technique and a novel thermal-gelation method. In this preparation process, the mixture of quaternized chitosan solution and alpha-beta-glycerophosphate (alpha-beta-GP) was used as water phase and dispersed in oil phase to form uniform W/O emulsion by SPG membrane emulsification technique. The droplets solidified into microspheres at 37 degrees C by thermal-gelation method. The whole process was simple and mild. The influence of process conditions on the property of prepared microspheres was investigated and the optimized preparation condition was obtained. As a result, the coefficient of variation (C.V.) of obtained microspheres diameters was below 15%. The obtained microsphere had porous structure and showed apparent pH-sensitivity. It dissolved rapidly in acid solution (pH 5) and kept stable in neutral solution (pH 7.4). The pH-sensitivity of microspheres also affected its drug release behavior. Bovine serum albumin (BSA) as a model drug was encapsulated in microspheres, and it was released rapidly in acid solution and slowly in neutral medium. The novel quaternized chitosan microspheres with pH-sensitivity can be used as drug delivery system in the biomedical field, such as tumor-targeted drug carrier. SN - 0927-7765 UR - https://www.unboundmedicine.com/medline/citation/18226510/Preparation_of_uniform_sized_pH_sensitive_quaternized_chitosan_microsphere_by_combining_membrane_emulsification_technique_and_thermal_gelation_method_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(07)00435-3 DB - PRIME DP - Unbound Medicine ER -