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Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene.
Antimicrob Agents Chemother. 2008 Apr; 52(4):1257-63.AA

Abstract

Genetic structures surrounding the carbapenem-hydrolyzing Ambler class A bla KPC gene were characterized in several KPC-positive Klebsiella pneumoniae and Pseudomonas aeruginosa strains isolated from the United States, Colombia, and Greece. The bla KPC genes were associated in all cases with transposon-related structures. In the K. pneumoniae YC isolate from the United States, the beta-lactamase bla KPC-2 gene was located on a novel Tn3-based transposon, Tn4401. Tn4401 was 10 kb in size, was delimited by two 39-bp imperfect inverted repeat sequences, and harbored, in addition to the beta-lactamase bla KPC-2 gene, a transposase gene, a resolvase gene, and two novel insertion sequences, ISKpn6 and ISKpn7. Tn4401 has been identified in all isolates. However, two isoforms of this transposon were found: Tn4401a was found in K. pneumoniae YC and K. pneumoniae GR from the United States and Greece, respectively, and differed by a 100-bp deletion, located just upstream of the bla KPC-2 gene, compared to the sequence of Tn4401b, which was found in the Colombian isolates. In all isolates tested, Tn4401 was flanked by a 5-bp target site duplication, the signature of a recent transposition event, and was inserted in different open reading frames located on plasmids that varied in size and nature. Tn4401 is likely at the origin of carbapenem-hydrolyzing beta-lactamase KPC mobilization to plasmids and its further insertion into various-sized plasmids identified in nonclonally related K. pneumoniae and P. aeruginosa isolates.

Authors+Show Affiliations

Service de Bactériologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre 94275, and Faculté de Médecine Paris-Sud, Paris, France. thierry.naas@bct.aphp.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18227185

Citation

Naas, Thierry, et al. "Genetic Structures at the Origin of Acquisition of the Beta-lactamase Bla KPC Gene." Antimicrobial Agents and Chemotherapy, vol. 52, no. 4, 2008, pp. 1257-63.
Naas T, Cuzon G, Villegas MV, et al. Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene. Antimicrob Agents Chemother. 2008;52(4):1257-63.
Naas, T., Cuzon, G., Villegas, M. V., Lartigue, M. F., Quinn, J. P., & Nordmann, P. (2008). Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene. Antimicrobial Agents and Chemotherapy, 52(4), 1257-63. https://doi.org/10.1128/AAC.01451-07
Naas T, et al. Genetic Structures at the Origin of Acquisition of the Beta-lactamase Bla KPC Gene. Antimicrob Agents Chemother. 2008;52(4):1257-63. PubMed PMID: 18227185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene. AU - Naas,Thierry, AU - Cuzon,Gaelle, AU - Villegas,Maria-Virginia, AU - Lartigue,Marie-Frédérique, AU - Quinn,John P, AU - Nordmann,Patrice, Y1 - 2008/01/28/ PY - 2008/1/30/pubmed PY - 2008/7/4/medline PY - 2008/1/30/entrez SP - 1257 EP - 63 JF - Antimicrobial agents and chemotherapy JO - Antimicrob Agents Chemother VL - 52 IS - 4 N2 - Genetic structures surrounding the carbapenem-hydrolyzing Ambler class A bla KPC gene were characterized in several KPC-positive Klebsiella pneumoniae and Pseudomonas aeruginosa strains isolated from the United States, Colombia, and Greece. The bla KPC genes were associated in all cases with transposon-related structures. In the K. pneumoniae YC isolate from the United States, the beta-lactamase bla KPC-2 gene was located on a novel Tn3-based transposon, Tn4401. Tn4401 was 10 kb in size, was delimited by two 39-bp imperfect inverted repeat sequences, and harbored, in addition to the beta-lactamase bla KPC-2 gene, a transposase gene, a resolvase gene, and two novel insertion sequences, ISKpn6 and ISKpn7. Tn4401 has been identified in all isolates. However, two isoforms of this transposon were found: Tn4401a was found in K. pneumoniae YC and K. pneumoniae GR from the United States and Greece, respectively, and differed by a 100-bp deletion, located just upstream of the bla KPC-2 gene, compared to the sequence of Tn4401b, which was found in the Colombian isolates. In all isolates tested, Tn4401 was flanked by a 5-bp target site duplication, the signature of a recent transposition event, and was inserted in different open reading frames located on plasmids that varied in size and nature. Tn4401 is likely at the origin of carbapenem-hydrolyzing beta-lactamase KPC mobilization to plasmids and its further insertion into various-sized plasmids identified in nonclonally related K. pneumoniae and P. aeruginosa isolates. SN - 0066-4804 UR - https://www.unboundmedicine.com/medline/citation/18227185/Genetic_structures_at_the_origin_of_acquisition_of_the_beta_lactamase_bla_KPC_gene_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=18227185 DB - PRIME DP - Unbound Medicine ER -