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Separation and determination of ephedrine and pseudoephedrine in Ephedrae Herba by CZE modified with a Cu(II)-L-lysine complex.
Electrophoresis. 2008 Feb; 29(3):658-64.E

Abstract

A CZE method using a complex of 2.5 mM Cu(II)-L-lysine (molar ratio is 1:2) as additive in a run buffer solution composed of Tris-H(3)PO(4) (pH 4.5) was developed for the simultaneous determination of ephedrine and pseudoephedrine within 4 min. The effects of pH, composition, and concentration of run buffer as well as the composition and concentration of the Cu(II)-L-lysine complex on the separation were investigated. The linear ranges for the determination of ephedrine and pseudoephedrine were 15.0-225.0 and 20.0-250.0 mg/L with LODs both of 5.0 mg/L. Satisfactory result for the determination of ephedrine and pseudoephedrine in Ephedrae Herba from different producing area was obtained by the proposed method. Ephedrine and pseudoephedrine were separated effectively with each other and with the other compounds in the sample. The RSD for the determination of the two constituents in the samples varied from 1.82 to 2.76%, and the recovery ranged between 95.0 and 104.0%.

Authors+Show Affiliations

Key Laboratory of Medicinal Chemistry for Nature Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University, Yunnan, P R China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18228536

Citation

Li, Fei, et al. "Separation and Determination of Ephedrine and Pseudoephedrine in Ephedrae Herba By CZE Modified With a Cu(II)-L-lysine Complex." Electrophoresis, vol. 29, no. 3, 2008, pp. 658-64.
Li F, Ding Z, Cao QE. Separation and determination of ephedrine and pseudoephedrine in Ephedrae Herba by CZE modified with a Cu(II)-L-lysine complex. Electrophoresis. 2008;29(3):658-64.
Li, F., Ding, Z., & Cao, Q. E. (2008). Separation and determination of ephedrine and pseudoephedrine in Ephedrae Herba by CZE modified with a Cu(II)-L-lysine complex. Electrophoresis, 29(3), 658-64. https://doi.org/10.1002/elps.200700334
Li F, Ding Z, Cao QE. Separation and Determination of Ephedrine and Pseudoephedrine in Ephedrae Herba By CZE Modified With a Cu(II)-L-lysine Complex. Electrophoresis. 2008;29(3):658-64. PubMed PMID: 18228536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of ephedrine and pseudoephedrine in Ephedrae Herba by CZE modified with a Cu(II)-L-lysine complex. AU - Li,Fei, AU - Ding,Zhongtao, AU - Cao,Qiu-E, PY - 2008/1/30/pubmed PY - 2008/4/18/medline PY - 2008/1/30/entrez SP - 658 EP - 64 JF - Electrophoresis JO - Electrophoresis VL - 29 IS - 3 N2 - A CZE method using a complex of 2.5 mM Cu(II)-L-lysine (molar ratio is 1:2) as additive in a run buffer solution composed of Tris-H(3)PO(4) (pH 4.5) was developed for the simultaneous determination of ephedrine and pseudoephedrine within 4 min. The effects of pH, composition, and concentration of run buffer as well as the composition and concentration of the Cu(II)-L-lysine complex on the separation were investigated. The linear ranges for the determination of ephedrine and pseudoephedrine were 15.0-225.0 and 20.0-250.0 mg/L with LODs both of 5.0 mg/L. Satisfactory result for the determination of ephedrine and pseudoephedrine in Ephedrae Herba from different producing area was obtained by the proposed method. Ephedrine and pseudoephedrine were separated effectively with each other and with the other compounds in the sample. The RSD for the determination of the two constituents in the samples varied from 1.82 to 2.76%, and the recovery ranged between 95.0 and 104.0%. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/18228536/Separation_and_determination_of_ephedrine_and_pseudoephedrine_in_Ephedrae_Herba_by_CZE_modified_with_a_Cu_II__L_lysine_complex_ L2 - https://doi.org/10.1002/elps.200700334 DB - PRIME DP - Unbound Medicine ER -