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Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liquid chromatography.
Phytochem Anal. 2008 Jan-Feb; 19(1):86-9.PA

Abstract

Two novel methods for the analysis of ellagic acid in pomegranate (Punica granatum) rinds are proposed. Capillary electrophoresis (CE) was performed in a bare fused-silica capillary using a buffer solution of tri(hydroxymethyl)aminomethane:potassium dihydrogen phosphate (pH 8.4) with an applied voltage of 20 kV and UV detection at 254 nm. HPLC analysis was performed with a Zobax SB C(18) column and a mobile phase consisting of methanol:ethyl acetate:potassium dihydrogen phosphate: phosphoric acid at a flow rate of 1.0 mL/min. Under optimised conditions, the HPLC retention and the CE migration times for ellagic acid were 10.32 and 12.23 min, respectively. Calibration curves of peak area vs. concentration gave correlation coefficients of 0.9999 for HPLC and 0.9990 for CE. The detection limits for HPLC and CE were 2.8 and 2.2 microg/mL, respectively. Average recoveries were 98.32 +/- 1.2% for HPLC and 96.52 +/- 2.8% for CE. Both methods were shown to be suitable for the determination of ellagic acid in pomegranate rinds extraction; however, the CE method required less solvent and gave better column efficiency, whilst the HPLC provided superior precision.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhou B
Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan City, Hubei Province, People's Republic of China. benhongz@yahoo.com.cn
Wu Z
No affiliation info available
Li X
No affiliation info available
Zhang J
No affiliation info available
Hu X
No affiliation info available

MeSH

Chromatography, High Pressure LiquidElectrophoresis, CapillaryEllagic AcidLythraceaeReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18229889

Citation

Zhou, Benhong, et al. "Analysis of Ellagic Acid in Pomegranate Rinds By Capillary Electrophoresis and High-performance Liquid Chromatography." Phytochemical Analysis : PCA, vol. 19, no. 1, 2008, pp. 86-9.
Zhou B, Wu Z, Li X, et al. Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liquid chromatography. Phytochem Anal. 2008;19(1):86-9.
Zhou, B., Wu, Z., Li, X., Zhang, J., & Hu, X. (2008). Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liquid chromatography. Phytochemical Analysis : PCA, 19(1), 86-9. https://doi.org/10.1002/pca.1054
Zhou B, et al. Analysis of Ellagic Acid in Pomegranate Rinds By Capillary Electrophoresis and High-performance Liquid Chromatography. Phytochem Anal. 2008 Jan-Feb;19(1):86-9. PubMed PMID: 18229889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liquid chromatography. AU - Zhou,Benhong, AU - Wu,Zhenhua, AU - Li,Xiaojun, AU - Zhang,Jie, AU - Hu,Xianming, PY - 2008/1/31/pubmed PY - 2008/4/11/medline PY - 2008/1/31/entrez SP - 86 EP - 9 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 19 IS - 1 N2 - Two novel methods for the analysis of ellagic acid in pomegranate (Punica granatum) rinds are proposed. Capillary electrophoresis (CE) was performed in a bare fused-silica capillary using a buffer solution of tri(hydroxymethyl)aminomethane:potassium dihydrogen phosphate (pH 8.4) with an applied voltage of 20 kV and UV detection at 254 nm. HPLC analysis was performed with a Zobax SB C(18) column and a mobile phase consisting of methanol:ethyl acetate:potassium dihydrogen phosphate: phosphoric acid at a flow rate of 1.0 mL/min. Under optimised conditions, the HPLC retention and the CE migration times for ellagic acid were 10.32 and 12.23 min, respectively. Calibration curves of peak area vs. concentration gave correlation coefficients of 0.9999 for HPLC and 0.9990 for CE. The detection limits for HPLC and CE were 2.8 and 2.2 microg/mL, respectively. Average recoveries were 98.32 +/- 1.2% for HPLC and 96.52 +/- 2.8% for CE. Both methods were shown to be suitable for the determination of ellagic acid in pomegranate rinds extraction; however, the CE method required less solvent and gave better column efficiency, whilst the HPLC provided superior precision. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/18229889/Analysis_of_ellagic_acid_in_pomegranate_rinds_by_capillary_electrophoresis_and_high_performance_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -