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Inhibition of insect calcium channels by huwentoxin-V, a neurotoxin from Chinese tarantula Ornithoctonus huwena venom.
Eur J Pharmacol. 2008 Mar 17; 582(1-3):12-6.EJ

Abstract

The effects of huwentoxin-V, an insect neurotoxic peptide from Chinese tarantula Ornithoctonus huwena venom, were studied on neuronal voltage-gated ion channels. Whole-cell patch-clamp configuration indicated that huwentoxin-V specifically inhibited high-voltage-activated calcium channels in adult cockroach dorsal unpaired median neurons (IC(50) approximately 219 nM) while having no evident effect on voltage-gated potassium and sodium channels. Omega-conotoxin GVIA is a well-known neuronal N-type calcium channel blocker from the venom of the sea snail Conus geographus and it also can partially block calcium currents in cockroach dorsal unpaired median neurons. In our study, huwentoxin-V inhibited omega-conotoxin GVIA-sensitive, diltiazem-sensitive and partial omega-conotoxin GVIA and diltiazem-resistant calcium currents elicited from insect neurons. Based on the sensitivity of calcium currents to these toxins, insect neuronal HVA calcium channels might be classified into four types: Type I, omega-conotoxin GVIA-sensitive and huwentoxin-V-sensitive; type II, diltiazem-sensitive and huwentoxin-V-sensitive; type III, huwentoxin-V-sensitive but omega-conotoxin GVIA and diltiazem-resistant; type IV, omega-conotoxin GVIA and diltiazem-resistant and huwentoxin-V-resistant. Its specificity, incomplete inhibition and insect-toxic effects make it an interesting tool for investigating insect voltage-gated calcium channels and development of new insecticidal compounds.

Authors+Show Affiliations

College of Life Sciences, Hunan Normal University, Changsha 410081, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18234186

Citation

Deng, Meichun, et al. "Inhibition of Insect Calcium Channels By huwentoxin-V, a Neurotoxin From Chinese Tarantula Ornithoctonus Huwena Venom." European Journal of Pharmacology, vol. 582, no. 1-3, 2008, pp. 12-6.
Deng M, Luo X, Meng E, et al. Inhibition of insect calcium channels by huwentoxin-V, a neurotoxin from Chinese tarantula Ornithoctonus huwena venom. Eur J Pharmacol. 2008;582(1-3):12-6.
Deng, M., Luo, X., Meng, E., Xiao, Y., & Liang, S. (2008). Inhibition of insect calcium channels by huwentoxin-V, a neurotoxin from Chinese tarantula Ornithoctonus huwena venom. European Journal of Pharmacology, 582(1-3), 12-6. https://doi.org/10.1016/j.ejphar.2007.12.014
Deng M, et al. Inhibition of Insect Calcium Channels By huwentoxin-V, a Neurotoxin From Chinese Tarantula Ornithoctonus Huwena Venom. Eur J Pharmacol. 2008 Mar 17;582(1-3):12-6. PubMed PMID: 18234186.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of insect calcium channels by huwentoxin-V, a neurotoxin from Chinese tarantula Ornithoctonus huwena venom. AU - Deng,Meichun, AU - Luo,Xuan, AU - Meng,Er, AU - Xiao,Yucheng, AU - Liang,Songping, Y1 - 2007/12/27/ PY - 2007/05/09/received PY - 2007/12/02/revised PY - 2007/12/16/accepted PY - 2008/2/1/pubmed PY - 2008/6/6/medline PY - 2008/2/1/entrez SP - 12 EP - 6 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 582 IS - 1-3 N2 - The effects of huwentoxin-V, an insect neurotoxic peptide from Chinese tarantula Ornithoctonus huwena venom, were studied on neuronal voltage-gated ion channels. Whole-cell patch-clamp configuration indicated that huwentoxin-V specifically inhibited high-voltage-activated calcium channels in adult cockroach dorsal unpaired median neurons (IC(50) approximately 219 nM) while having no evident effect on voltage-gated potassium and sodium channels. Omega-conotoxin GVIA is a well-known neuronal N-type calcium channel blocker from the venom of the sea snail Conus geographus and it also can partially block calcium currents in cockroach dorsal unpaired median neurons. In our study, huwentoxin-V inhibited omega-conotoxin GVIA-sensitive, diltiazem-sensitive and partial omega-conotoxin GVIA and diltiazem-resistant calcium currents elicited from insect neurons. Based on the sensitivity of calcium currents to these toxins, insect neuronal HVA calcium channels might be classified into four types: Type I, omega-conotoxin GVIA-sensitive and huwentoxin-V-sensitive; type II, diltiazem-sensitive and huwentoxin-V-sensitive; type III, huwentoxin-V-sensitive but omega-conotoxin GVIA and diltiazem-resistant; type IV, omega-conotoxin GVIA and diltiazem-resistant and huwentoxin-V-resistant. Its specificity, incomplete inhibition and insect-toxic effects make it an interesting tool for investigating insect voltage-gated calcium channels and development of new insecticidal compounds. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18234186/Inhibition_of_insect_calcium_channels_by_huwentoxin_V_a_neurotoxin_from_Chinese_tarantula_Ornithoctonus_huwena_venom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)01351-9 DB - PRIME DP - Unbound Medicine ER -