TY - JOUR T1 - Structural characterization of the cell wall D-glucans isolated from the mycelium of Botryosphaeria rhodina MAMB-05. AU - de Lourdes Corradi da Silva,Maria, AU - Fukuda,Eliane K, AU - Vasconcelos,Ana Flora D, AU - Dekker,Robert F H, AU - Matias,Andreza C, AU - Monteiro,Nilson K, AU - Cardoso,Marilsa S, AU - Barbosa,Aneli M, AU - Silveira,Joana L M, AU - Sassaki,Guilherme L, AU - Carbonero,Elaine R, Y1 - 2008/01/11/ PY - 2007/10/26/received PY - 2007/12/18/revised PY - 2007/12/22/accepted PY - 2008/2/2/pubmed PY - 2008/6/13/medline PY - 2008/2/2/entrez SP - 793 EP - 8 JF - Carbohydrate research JO - Carbohydr Res VL - 343 IS - 4 N2 - Three D-glucans were isolated from the mycelium of the fungus Botryosphaeria rhodina MAMB-05 by sequential extraction with hot-water and hot aqueous KOH (2% w/v) followed by ethanol precipitation. Following their purification by gel permeation chromatography on Sepharose CL-4B, the structural characteristics of the D-glucans were determined by FT-IR and 13C NMR spectroscopy and, after methylation, by GC-MS. The hot-water extract produced a fraction designated Q1A that was a beta-(1-->6)-D-glucan with the following structure: [Formula: see text] The alkaline extract, when subjected to repeated freeze-thawing, yielded two fractions: K1P (insoluble) that comprised a beta-(1-->3)-D-glucan with beta-D-glucose branches at C-6 with the structure: [Formula: see text] and K1SA (soluble) consisting of a backbone chain of alpha-(1-->4)-linked D-glucopyranosyl residues substituted at O-6 with alpha-D-glucopyranosyl residues: [Formula: see text] SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/18237722/Structural_characterization_of_the_cell_wall_D_glucans_isolated_from_the_mycelium_of_Botryosphaeria_rhodina_MAMB_05_ DB - PRIME DP - Unbound Medicine ER -