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Ex vivo priming of endothelial progenitor cells with SDF-1 before transplantation could increase their proangiogenic potential.
Arterioscler Thromb Vasc Biol. 2008 Apr; 28(4):644-50.AT

Abstract

OBJECTIVE

As SDF-1 and its cognate receptor CXCR4 play a key role in the survival and mobilization of immature cells, we examined whether preconditioning of endothelial progenitor cells (EPCs) with SDF-1 could further promote their capacity to enhance angiogenesis.

METHODS AND RESULTS

EPC exposure to 100 ng/mL SDF-1 for 30 min induced a proangiogenic phenotype, with cell migration and differentiation into vascular cords in Matrigel and increased their therapeutic potential in a nude mouse model of hindlimb ischemia. This pretreatment enhanced EPC adhesion to activated endothelium in physiological conditions of blood flow by stimulating integrin-mediated EPCs binding to endothelial cells. Pretreated EPCs showed significantly upregulated surface alpha4 and alphaM integrin subunit expression involved in the homing of immature cells to a neovasculature and enhanced FGF-2 and promatrix metalloproteinase (MMP)-2 secretion. All these effects were significantly attenuated by EPC incubation with AMD-3100, a CXCR4 antagonist, by prior HSPGs disruption and by HUVEC incubation with anti-intercellular adhesion molecule1 (ICAM-1) and anti-vascular cell adhesion molecule (VCAM) blocking antibodies. Pretreated EPCs adhered very rapidly (within minutes) and were resistant to shear stresses of up to 2500 s(-1).

CONCLUSIONS

SDF-1 pretreatment during EPC expansion stimulates EPC adhesion to endothelial cells and thus augments the efficiency of cell therapy for ischemic vascular diseases.

Authors+Show Affiliations

INSERM U765, Cardiovascular Research Center, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18239152

Citation

Zemani, Faouzia, et al. "Ex Vivo Priming of Endothelial Progenitor Cells With SDF-1 Before Transplantation Could Increase Their Proangiogenic Potential." Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 28, no. 4, 2008, pp. 644-50.
Zemani F, Silvestre JS, Fauvel-Lafeve F, et al. Ex vivo priming of endothelial progenitor cells with SDF-1 before transplantation could increase their proangiogenic potential. Arterioscler Thromb Vasc Biol. 2008;28(4):644-50.
Zemani, F., Silvestre, J. S., Fauvel-Lafeve, F., Bruel, A., Vilar, J., Bieche, I., Laurendeau, I., Galy-Fauroux, I., Fischer, A. M., & Boisson-Vidal, C. (2008). Ex vivo priming of endothelial progenitor cells with SDF-1 before transplantation could increase their proangiogenic potential. Arteriosclerosis, Thrombosis, and Vascular Biology, 28(4), 644-50. https://doi.org/10.1161/ATVBAHA.107.160044
Zemani F, et al. Ex Vivo Priming of Endothelial Progenitor Cells With SDF-1 Before Transplantation Could Increase Their Proangiogenic Potential. Arterioscler Thromb Vasc Biol. 2008;28(4):644-50. PubMed PMID: 18239152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ex vivo priming of endothelial progenitor cells with SDF-1 before transplantation could increase their proangiogenic potential. AU - Zemani,Faouzia, AU - Silvestre,Jean-Sébastien, AU - Fauvel-Lafeve,Françoise, AU - Bruel,Arlette, AU - Vilar,José, AU - Bieche,Ivan, AU - Laurendeau,Ingrid, AU - Galy-Fauroux,Isabelle, AU - Fischer,Anne Marie, AU - Boisson-Vidal,Catherine, Y1 - 2008/01/31/ PY - 2008/2/2/pubmed PY - 2008/4/22/medline PY - 2008/2/2/entrez SP - 644 EP - 50 JF - Arteriosclerosis, thrombosis, and vascular biology JO - Arterioscler Thromb Vasc Biol VL - 28 IS - 4 N2 - OBJECTIVE: As SDF-1 and its cognate receptor CXCR4 play a key role in the survival and mobilization of immature cells, we examined whether preconditioning of endothelial progenitor cells (EPCs) with SDF-1 could further promote their capacity to enhance angiogenesis. METHODS AND RESULTS: EPC exposure to 100 ng/mL SDF-1 for 30 min induced a proangiogenic phenotype, with cell migration and differentiation into vascular cords in Matrigel and increased their therapeutic potential in a nude mouse model of hindlimb ischemia. This pretreatment enhanced EPC adhesion to activated endothelium in physiological conditions of blood flow by stimulating integrin-mediated EPCs binding to endothelial cells. Pretreated EPCs showed significantly upregulated surface alpha4 and alphaM integrin subunit expression involved in the homing of immature cells to a neovasculature and enhanced FGF-2 and promatrix metalloproteinase (MMP)-2 secretion. All these effects were significantly attenuated by EPC incubation with AMD-3100, a CXCR4 antagonist, by prior HSPGs disruption and by HUVEC incubation with anti-intercellular adhesion molecule1 (ICAM-1) and anti-vascular cell adhesion molecule (VCAM) blocking antibodies. Pretreated EPCs adhered very rapidly (within minutes) and were resistant to shear stresses of up to 2500 s(-1). CONCLUSIONS: SDF-1 pretreatment during EPC expansion stimulates EPC adhesion to endothelial cells and thus augments the efficiency of cell therapy for ischemic vascular diseases. SN - 1524-4636 UR - https://www.unboundmedicine.com/medline/citation/18239152/Ex_vivo_priming_of_endothelial_progenitor_cells_with_SDF_1_before_transplantation_could_increase_their_proangiogenic_potential_ L2 - https://www.ahajournals.org/doi/10.1161/ATVBAHA.107.160044?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -