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Molecular authentication and quality control using a high performance liquid chromatography technique of Fructus Evodiae.
Biol Pharm Bull. 2008 Feb; 31(2):312-5.BP

Abstract

In present paper, the properties of molecular authentication combined with the fingerprints of high performance liquid chromatography (HPLC) were validated by analyzing ten batches of Fructus Evodiae samples (the dried nearly ripe fruit of Evodia rutaecarpa (JUSS.) BENTH., Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG or Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG). The results of this investigation show that the similarities of internal transcribed spacer (ITS) sequences were almost 100% in Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG, 97% in Evodia rutaecarpa (JUSS.) BENTH., and 96% in Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG. The percentage of identity between the two groups of Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG and Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG is almost 96%, but the identity among the group of these three species is only 73%. The results show that Fructus Evodiae comes from three species respectively. The fingerprints of HPLC show that Fructus Evodiae revealed 20 major common peaks. And the three species have almost the same chemical constituents. ITS sequence fingerprint combining the fingerprint of HPLC can not only be developed to identify and distinguish the three species in detail, but also can be used for optimizing location where Fructus Evodiae has much higher bioactive constituents and yield.

Authors+Show Affiliations

Provincial Key Laboratory of Drug Research, Hunan Academy of Traditional Chinese Medicine, Changsha, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18239294

Citation

Huang, Dan, et al. "Molecular Authentication and Quality Control Using a High Performance Liquid Chromatography Technique of Fructus Evodiae." Biological & Pharmaceutical Bulletin, vol. 31, no. 2, 2008, pp. 312-5.
Huang D, Li SX, Cai GX, et al. Molecular authentication and quality control using a high performance liquid chromatography technique of Fructus Evodiae. Biol Pharm Bull. 2008;31(2):312-5.
Huang, D., Li, S. X., Cai, G. X., Yue, C. H., Wei, L. J., & Zhang, P. (2008). Molecular authentication and quality control using a high performance liquid chromatography technique of Fructus Evodiae. Biological & Pharmaceutical Bulletin, 31(2), 312-5.
Huang D, et al. Molecular Authentication and Quality Control Using a High Performance Liquid Chromatography Technique of Fructus Evodiae. Biol Pharm Bull. 2008;31(2):312-5. PubMed PMID: 18239294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular authentication and quality control using a high performance liquid chromatography technique of Fructus Evodiae. AU - Huang,Dan, AU - Li,Shun Xiang, AU - Cai,Guang Xian, AU - Yue,Chun Hua, AU - Wei,Li Jun, AU - Zhang,Ping, PY - 2008/2/2/pubmed PY - 2008/3/11/medline PY - 2008/2/2/entrez SP - 312 EP - 5 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 31 IS - 2 N2 - In present paper, the properties of molecular authentication combined with the fingerprints of high performance liquid chromatography (HPLC) were validated by analyzing ten batches of Fructus Evodiae samples (the dried nearly ripe fruit of Evodia rutaecarpa (JUSS.) BENTH., Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG or Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG). The results of this investigation show that the similarities of internal transcribed spacer (ITS) sequences were almost 100% in Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG, 97% in Evodia rutaecarpa (JUSS.) BENTH., and 96% in Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG. The percentage of identity between the two groups of Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG and Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG is almost 96%, but the identity among the group of these three species is only 73%. The results show that Fructus Evodiae comes from three species respectively. The fingerprints of HPLC show that Fructus Evodiae revealed 20 major common peaks. And the three species have almost the same chemical constituents. ITS sequence fingerprint combining the fingerprint of HPLC can not only be developed to identify and distinguish the three species in detail, but also can be used for optimizing location where Fructus Evodiae has much higher bioactive constituents and yield. SN - 0918-6158 UR - https://www.unboundmedicine.com/medline/citation/18239294/Molecular_authentication_and_quality_control_using_a_high_performance_liquid_chromatography_technique_of_Fructus_Evodiae_ DB - PRIME DP - Unbound Medicine ER -