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Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle.
Vet Microbiol. 2008 Jul 27; 130(1-2):141-50.VM

Abstract

Bovine digital dermatitis (BDD) is a severe infectious cause of lameness which has spread through dairy cattle populations worldwide, causing serious welfare and agricultural problems. Spirochetes are the main organisms implicated and have previously proven difficult to isolate. This study aimed to isolate and characterise the range of spirochetes associated with BDD in the UK. Twenty-three spirochete isolates were obtained from 30 BDD lesions, which by 16S rRNA gene and flaB2 gene analysis clustered within the genus Treponema as three phylogroups; groups 1 (Treponema medium/Treponema vincentii-like), 2 (Treponema phagedenis-like) and 3 (Treponema denticola/Treponema putidum-like). The treponemes displayed large genotypic and phenotypic diversity between phylogroups and differed from named treponeme species. A previously isolated contagious ovine digital dermatitis spirochete was located within one of the three phylogroups, group 3, and could also be identified within this group on the basis of phenotype testing, suggesting BDD and contagious ovine digital dermatitis may share the same aetiological agent. A strain isolated from a bovine interdigital dermatitis lesion, could be identified as part of BDD isolate group 2, suggesting bovine interdigital dermatitis and BDD may have the same causative agent. Two common enzyme activities, C4 esterase and C8 esterase lipase, were identified in all BDD associated treponemes suggesting common metabolic pathways for sharing this novel niche or even common virulence traits. Further studies are required to determine whether the three groups of novel treponemes are representative of new treponeme taxa and to delineate how they interact with bovine tissues to cause disease.

Authors+Show Affiliations

Department of Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 3BX, UK. evansnj@liverpool.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18243592

Citation

Evans, Nicholas J., et al. "Three Unique Groups of Spirochetes Isolated From Digital Dermatitis Lesions in UK Cattle." Veterinary Microbiology, vol. 130, no. 1-2, 2008, pp. 141-50.
Evans NJ, Brown JM, Demirkan I, et al. Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle. Vet Microbiol. 2008;130(1-2):141-50.
Evans, N. J., Brown, J. M., Demirkan, I., Murray, R. D., Vink, W. D., Blowey, R. W., Hart, C. A., & Carter, S. D. (2008). Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle. Veterinary Microbiology, 130(1-2), 141-50. https://doi.org/10.1016/j.vetmic.2007.12.019
Evans NJ, et al. Three Unique Groups of Spirochetes Isolated From Digital Dermatitis Lesions in UK Cattle. Vet Microbiol. 2008 Jul 27;130(1-2):141-50. PubMed PMID: 18243592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle. AU - Evans,Nicholas J, AU - Brown,Jennifer M, AU - Demirkan,Ibrahim, AU - Murray,Richard D, AU - Vink,W Daan, AU - Blowey,Roger W, AU - Hart,C Anthony, AU - Carter,Stuart D, Y1 - 2008/01/04/ PY - 2007/11/05/received PY - 2007/12/20/revised PY - 2007/12/28/accepted PY - 2008/2/5/pubmed PY - 2008/10/29/medline PY - 2008/2/5/entrez SP - 141 EP - 50 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 130 IS - 1-2 N2 - Bovine digital dermatitis (BDD) is a severe infectious cause of lameness which has spread through dairy cattle populations worldwide, causing serious welfare and agricultural problems. Spirochetes are the main organisms implicated and have previously proven difficult to isolate. This study aimed to isolate and characterise the range of spirochetes associated with BDD in the UK. Twenty-three spirochete isolates were obtained from 30 BDD lesions, which by 16S rRNA gene and flaB2 gene analysis clustered within the genus Treponema as three phylogroups; groups 1 (Treponema medium/Treponema vincentii-like), 2 (Treponema phagedenis-like) and 3 (Treponema denticola/Treponema putidum-like). The treponemes displayed large genotypic and phenotypic diversity between phylogroups and differed from named treponeme species. A previously isolated contagious ovine digital dermatitis spirochete was located within one of the three phylogroups, group 3, and could also be identified within this group on the basis of phenotype testing, suggesting BDD and contagious ovine digital dermatitis may share the same aetiological agent. A strain isolated from a bovine interdigital dermatitis lesion, could be identified as part of BDD isolate group 2, suggesting bovine interdigital dermatitis and BDD may have the same causative agent. Two common enzyme activities, C4 esterase and C8 esterase lipase, were identified in all BDD associated treponemes suggesting common metabolic pathways for sharing this novel niche or even common virulence traits. Further studies are required to determine whether the three groups of novel treponemes are representative of new treponeme taxa and to delineate how they interact with bovine tissues to cause disease. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/18243592/Three_unique_groups_of_spirochetes_isolated_from_digital_dermatitis_lesions_in_UK_cattle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(08)00002-3 DB - PRIME DP - Unbound Medicine ER -