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A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae.
Nat Methods. 2008 Mar; 5(3):239-41.NM

Abstract

Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Jones GM
Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
Stalker J
No affiliation info available
Humphray S
No affiliation info available
West A
No affiliation info available
Cox T
No affiliation info available
Rogers J
No affiliation info available
Dunham I
No affiliation info available
Prelich G
No affiliation info available

MeSH

Gene Expression ProfilingGene Expression Regulation, FungalGene LibraryGenes, FungalGenome, FungalSaccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

18246075

Citation

Jones, Grace Marie, et al. "A Systematic Library for Comprehensive Overexpression Screens in Saccharomyces Cerevisiae." Nature Methods, vol. 5, no. 3, 2008, pp. 239-41.
Jones GM, Stalker J, Humphray S, et al. A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae. Nat Methods. 2008;5(3):239-41.
Jones, G. M., Stalker, J., Humphray, S., West, A., Cox, T., Rogers, J., Dunham, I., & Prelich, G. (2008). A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae. Nature Methods, 5(3), 239-41. https://doi.org/10.1038/nmeth.1181
Jones GM, et al. A Systematic Library for Comprehensive Overexpression Screens in Saccharomyces Cerevisiae. Nat Methods. 2008;5(3):239-41. PubMed PMID: 18246075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae. AU - Jones,Grace Marie, AU - Stalker,Jim, AU - Humphray,Sean, AU - West,Anthony, AU - Cox,Tony, AU - Rogers,Jane, AU - Dunham,Ian, AU - Prelich,Gregory, Y1 - 2008/02/03/ PY - 2007/09/20/received PY - 2008/01/08/accepted PY - 2008/2/5/pubmed PY - 2008/3/14/medline PY - 2008/2/5/entrez SP - 239 EP - 41 JF - Nature methods JO - Nat Methods VL - 5 IS - 3 N2 - Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens. SN - 1548-7105 UR - https://www.unboundmedicine.com/medline/citation/18246075/A_systematic_library_for_comprehensive_overexpression_screens_in_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -