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Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium.
Biotechnol J. 2008 Feb; 3(2):245-51.BJ

Abstract

Probiotic bacteria synthesize extracellular polysaccharides (EPSs) with commercially significant physiological and therapeutic activities. This important class of biomolecules is also characterized by their ability to remove reactive oxygen species (ROS) that are formed in the intestine by various metabolic reactions; hence, they exhibit antioxidant activities. Our probiotic bacterium, Bacillus coagulans RK-02, produces an EPS during the exponential and stationary growth phases when grown in a glucose mineral salts medium. The time course of EPS synthesis was studied with respect to biomass growth. The antioxidant and free radical scavenging potential of isolated EPS were studied by various methods, including the beta-carotene-linoleic acid model system, a superoxide radical scavenging assay using the PMS-NADH-nitroblue tetrazolium system, the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, a hydroxyl radical scavenging assay using the ascorbic acid-Cu(2+)-cytochrome c system and an in vitro microsome peroxidation inhibition study using a thiobarbituric acid assay. The antioxidant activities were compared to known antioxidants vitamin C and E, which were used as reference standards. The results showed that the EPS, which is a heteropolymer composed of four monosaccharides, produced by B. coagulans RK-02 had significant antioxidant and free radical scavenging activities.

Authors+Show Affiliations

Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18246578

Citation

Kodali, Vidya Prabhakar, and Ramkrishna Sen. "Antioxidant and Free Radical Scavenging Activities of an Exopolysaccharide From a Probiotic Bacterium." Biotechnology Journal, vol. 3, no. 2, 2008, pp. 245-51.
Kodali VP, Sen R. Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. Biotechnol J. 2008;3(2):245-51.
Kodali, V. P., & Sen, R. (2008). Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. Biotechnology Journal, 3(2), 245-51. https://doi.org/10.1002/biot.200700208
Kodali VP, Sen R. Antioxidant and Free Radical Scavenging Activities of an Exopolysaccharide From a Probiotic Bacterium. Biotechnol J. 2008;3(2):245-51. PubMed PMID: 18246578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. AU - Kodali,Vidya Prabhakar, AU - Sen,Ramkrishna, PY - 2008/2/5/pubmed PY - 2008/3/28/medline PY - 2008/2/5/entrez SP - 245 EP - 51 JF - Biotechnology journal JO - Biotechnol J VL - 3 IS - 2 N2 - Probiotic bacteria synthesize extracellular polysaccharides (EPSs) with commercially significant physiological and therapeutic activities. This important class of biomolecules is also characterized by their ability to remove reactive oxygen species (ROS) that are formed in the intestine by various metabolic reactions; hence, they exhibit antioxidant activities. Our probiotic bacterium, Bacillus coagulans RK-02, produces an EPS during the exponential and stationary growth phases when grown in a glucose mineral salts medium. The time course of EPS synthesis was studied with respect to biomass growth. The antioxidant and free radical scavenging potential of isolated EPS were studied by various methods, including the beta-carotene-linoleic acid model system, a superoxide radical scavenging assay using the PMS-NADH-nitroblue tetrazolium system, the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, a hydroxyl radical scavenging assay using the ascorbic acid-Cu(2+)-cytochrome c system and an in vitro microsome peroxidation inhibition study using a thiobarbituric acid assay. The antioxidant activities were compared to known antioxidants vitamin C and E, which were used as reference standards. The results showed that the EPS, which is a heteropolymer composed of four monosaccharides, produced by B. coagulans RK-02 had significant antioxidant and free radical scavenging activities. SN - 1860-7314 UR - https://www.unboundmedicine.com/medline/citation/18246578/Antioxidant_and_free_radical_scavenging_activities_of_an_exopolysaccharide_from_a_probiotic_bacterium_ L2 - https://doi.org/10.1002/biot.200700208 DB - PRIME DP - Unbound Medicine ER -