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Tonic facilitation of glutamate release by glycine binding sites on presynaptic NR2B-containing NMDA autoreceptors in the rat visual cortex.
Neurosci Lett. 2008 Feb 27; 432(3):212-6.NL

Abstract

We have previously shown that glycine binding sites on presynaptic NMDA receptors (NMDA-Rs) can tonically regulate glutamate release in the rat visual cortex. In the present study, we investigated the subunit composition of these presynaptic NMDA-Rs. We recorded miniature a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor-mediated excitatory postsynaptic currents (mEPSCs) using whole-cell voltage clamp in layer II/III pyramidal neurons of the rat visual cortex with the open-channel NMDA receptor blocker, MK-801, in the recording pipette. We found that the frequency of mEPSCs is significantly reduced by 7-chloro-kynurenic acid (7-Cl KYNA) an NMDA-R glycine binding site antagonist, and glycine reverses this effect. Using a specific antagonist for NR2B-NMDA-Rs, Ro 25-6981 [(alphaR,betaS)-alpha-(4-hydroxyphenyl)-beta-methyl-4-(phenylmethyl)-1-piperidinepropanol hydrochloride], instead of 7-Cl KYNA, we found that the frequency of mEPSCs is also significantly reduced but glycine cannot reverse this effect. Moreover, Zn(2+), an NR2A-NMDA-R antagonist, did not affect mEPSC frequency. These results suggest that presynaptic NR2B-containing NMDA-Rs are located in layer II/III pyramidal neurons of the rat visual cortex, and that the glycine binding site of these type NMDA-Rs tonically regulates glutamate release.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li YH
Department of Physiology, School of Medicine, Xi'an Jiaotong University, Zhuque Dajie 205, Xi'an 710061, PR China.
Han TZ
No affiliation info available
Meng K
No affiliation info available

MeSH

AnimalsAnimals, NewbornBinding SitesDizocilpine MaleateDrug InteractionsExcitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsGlutamic AcidGlycineIn Vitro TechniquesKynurenic AcidPatch-Clamp TechniquesPhenolsPiperidinesPresynaptic TerminalsRatsRats, Sprague-DawleyReceptors, N-Methyl-D-AspartateVisual Cortex

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18248890

Citation

Li, Yan-Hai, et al. "Tonic Facilitation of Glutamate Release By Glycine Binding Sites On Presynaptic NR2B-containing NMDA Autoreceptors in the Rat Visual Cortex." Neuroscience Letters, vol. 432, no. 3, 2008, pp. 212-6.
Li YH, Han TZ, Meng K. Tonic facilitation of glutamate release by glycine binding sites on presynaptic NR2B-containing NMDA autoreceptors in the rat visual cortex. Neurosci Lett. 2008;432(3):212-6.
Li, Y. H., Han, T. Z., & Meng, K. (2008). Tonic facilitation of glutamate release by glycine binding sites on presynaptic NR2B-containing NMDA autoreceptors in the rat visual cortex. Neuroscience Letters, 432(3), 212-6. https://doi.org/10.1016/j.neulet.2007.12.023
Li YH, Han TZ, Meng K. Tonic Facilitation of Glutamate Release By Glycine Binding Sites On Presynaptic NR2B-containing NMDA Autoreceptors in the Rat Visual Cortex. Neurosci Lett. 2008 Feb 27;432(3):212-6. PubMed PMID: 18248890.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tonic facilitation of glutamate release by glycine binding sites on presynaptic NR2B-containing NMDA autoreceptors in the rat visual cortex. AU - Li,Yan-Hai, AU - Han,Tai-Zhen, AU - Meng,Kai, Y1 - 2007/12/23/ PY - 2007/07/13/received PY - 2007/11/28/revised PY - 2007/12/05/accepted PY - 2008/2/6/pubmed PY - 2008/6/13/medline PY - 2008/2/6/entrez SP - 212 EP - 6 JF - Neuroscience letters JO - Neurosci Lett VL - 432 IS - 3 N2 - We have previously shown that glycine binding sites on presynaptic NMDA receptors (NMDA-Rs) can tonically regulate glutamate release in the rat visual cortex. In the present study, we investigated the subunit composition of these presynaptic NMDA-Rs. We recorded miniature a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor-mediated excitatory postsynaptic currents (mEPSCs) using whole-cell voltage clamp in layer II/III pyramidal neurons of the rat visual cortex with the open-channel NMDA receptor blocker, MK-801, in the recording pipette. We found that the frequency of mEPSCs is significantly reduced by 7-chloro-kynurenic acid (7-Cl KYNA) an NMDA-R glycine binding site antagonist, and glycine reverses this effect. Using a specific antagonist for NR2B-NMDA-Rs, Ro 25-6981 [(alphaR,betaS)-alpha-(4-hydroxyphenyl)-beta-methyl-4-(phenylmethyl)-1-piperidinepropanol hydrochloride], instead of 7-Cl KYNA, we found that the frequency of mEPSCs is also significantly reduced but glycine cannot reverse this effect. Moreover, Zn(2+), an NR2A-NMDA-R antagonist, did not affect mEPSC frequency. These results suggest that presynaptic NR2B-containing NMDA-Rs are located in layer II/III pyramidal neurons of the rat visual cortex, and that the glycine binding site of these type NMDA-Rs tonically regulates glutamate release. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/18248890/Tonic_facilitation_of_glutamate_release_by_glycine_binding_sites_on_presynaptic_NR2B_containing_NMDA_autoreceptors_in_the_rat_visual_cortex_ DB - PRIME DP - Unbound Medicine ER -