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Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations.
Respir Med. 2008 May; 102(5):720-5.RM

Abstract

BACKGROUND

Exhaled breath condensate analysis is an attractive but still not fully standardised method for investigating airway pathology. Adherence of biomarkers to various condensing surfaces and changes in condensing temperature has been considered to be responsible for the variability of the results. Our aims were to compare the efficacy of different types of condensers and to test the influence of condensing temperature on condensate composition.

METHODS

Breath condensates from 12 healthy persons were collected in two settings: (1) by using three condensers of different type (EcoScreen, R-Tube, Anacon) and (2) by using R-Tube condenser either cooled to -20 or -70 degrees C. Condensate pH at standardised CO(2) level was determined; protein content was measured by the Bradford method and leukotrienes by EIA.

RESULTS

Breath condensates collected using EcoScreen were more alkaline (6.45+/-0.20 vs. 6.19+/-0.23, p<0.05 and 6.10+/-0.26, p<0.001) and contained more protein (3.89+/-2.03 vs. 2.65+/-1.98, n.s. and 1.88+/-1.99 microg/ml, p<0.004) as compared to the other devices. Only parameters obtained with R-Tube and Anacon correlated. Condensing temperature affected condensate pH (5.99+/-0.20 at -20 degrees C and 5.82+/-0.07 at -70 degrees C, p<0.05) but not protein content. Leukotriene B(4) was not found in any sample and cysteinyl-leukotriene was not found in condensates collected with R-Tube or Anacon.

CONCLUSION

Condenser type influences sample pH, total protein content and cysteinyl-leukotriene concentration. Condensing temperature influences condensate pH but not total protein content. These results suggest that adherence of the biomarkers to condenser surface and condensing temperature may play a role but does not fully explain the variability of EBC biomarker levels.

Authors+Show Affiliations

Department of Pathophysiology, National Korányi Institute for TB and Pulmonology, 1529 Budapest Pihenö u. 1, Budapest, Hungary.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18249106

Citation

Czebe, Krisztina, et al. "Influence of Condensing Equipment and Temperature On Exhaled Breath Condensate pH, Total Protein and Leukotriene Concentrations." Respiratory Medicine, vol. 102, no. 5, 2008, pp. 720-5.
Czebe K, Barta I, Antus B, et al. Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations. Respir Med. 2008;102(5):720-5.
Czebe, K., Barta, I., Antus, B., Valyon, M., Horváth, I., & Kullmann, T. (2008). Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations. Respiratory Medicine, 102(5), 720-5. https://doi.org/10.1016/j.rmed.2007.12.013
Czebe K, et al. Influence of Condensing Equipment and Temperature On Exhaled Breath Condensate pH, Total Protein and Leukotriene Concentrations. Respir Med. 2008;102(5):720-5. PubMed PMID: 18249106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of condensing equipment and temperature on exhaled breath condensate pH, total protein and leukotriene concentrations. AU - Czebe,Krisztina, AU - Barta,Imre, AU - Antus,Balázs, AU - Valyon,Márta, AU - Horváth,Ildikó, AU - Kullmann,Tamás, Y1 - 2008/01/31/ PY - 2007/09/18/received PY - 2007/12/18/revised PY - 2007/12/18/accepted PY - 2008/2/6/pubmed PY - 2008/8/12/medline PY - 2008/2/6/entrez SP - 720 EP - 5 JF - Respiratory medicine JO - Respir Med VL - 102 IS - 5 N2 - BACKGROUND: Exhaled breath condensate analysis is an attractive but still not fully standardised method for investigating airway pathology. Adherence of biomarkers to various condensing surfaces and changes in condensing temperature has been considered to be responsible for the variability of the results. Our aims were to compare the efficacy of different types of condensers and to test the influence of condensing temperature on condensate composition. METHODS: Breath condensates from 12 healthy persons were collected in two settings: (1) by using three condensers of different type (EcoScreen, R-Tube, Anacon) and (2) by using R-Tube condenser either cooled to -20 or -70 degrees C. Condensate pH at standardised CO(2) level was determined; protein content was measured by the Bradford method and leukotrienes by EIA. RESULTS: Breath condensates collected using EcoScreen were more alkaline (6.45+/-0.20 vs. 6.19+/-0.23, p<0.05 and 6.10+/-0.26, p<0.001) and contained more protein (3.89+/-2.03 vs. 2.65+/-1.98, n.s. and 1.88+/-1.99 microg/ml, p<0.004) as compared to the other devices. Only parameters obtained with R-Tube and Anacon correlated. Condensing temperature affected condensate pH (5.99+/-0.20 at -20 degrees C and 5.82+/-0.07 at -70 degrees C, p<0.05) but not protein content. Leukotriene B(4) was not found in any sample and cysteinyl-leukotriene was not found in condensates collected with R-Tube or Anacon. CONCLUSION: Condenser type influences sample pH, total protein content and cysteinyl-leukotriene concentration. Condensing temperature influences condensate pH but not total protein content. These results suggest that adherence of the biomarkers to condenser surface and condensing temperature may play a role but does not fully explain the variability of EBC biomarker levels. SN - 0954-6111 UR - https://www.unboundmedicine.com/medline/citation/18249106/Influence_of_condensing_equipment_and_temperature_on_exhaled_breath_condensate_pH_total_protein_and_leukotriene_concentrations_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(07)00512-4 DB - PRIME DP - Unbound Medicine ER -