Tags

Type your tag names separated by a space and hit enter

Lipid peroxidation and vitamin E in serum and follicular fluid of infertile women with peritoneal endometriosis submitted to controlled ovarian hyperstimulation: a pilot study.
Fertil Steril. 2008 Dec; 90(6):2080-5.FS

Abstract

OBJECTIVE

To assess the level of lipid peroxidation (LP) and vitamin E in the follicular fluid and serum of infertile patients, with or without endometriosis, who were submitted to ovulation induction for assisted reproduction procedures.

DESIGN

Prospective study.

SETTING

Assisted conception unit, university hospital.

PATIENT(S)

Infertile patients 20 to 38 years of age were selected prospectively and consecutively and were divided into the endometriosis group (17 patients with pelvic endometriosis) and the control group (19 patients with previous tubal ligation or male factor and without endometriosis).

INTERVENTION(S)

Peripheral blood samples were collected on D1 (before the beginning of the use of gonadotropins), D2 (day of hCG administration), and D3 (day of oocyte retrieval). On D3, follicular-fluid samples free from blood contamination also were collected and stored.

MAIN OUTCOME MEASURE(S)

Lipid peroxidation was assessed by malondialdehyde quantification by spectrophotometry, and measurement of vitamin E was performed by HLPC.

RESULT(S)

On D1, no significant difference in LP was observed between groups. However, vitamin E levels were significantly higher in the control group. On D2, LP levels were significantly higher in the endometriosis group compared with in the control group, and vitamin E levels continued to be significantly higher in the control group. On D3, there was no significant difference in serum and follicular-fluid levels of LP and vitamin E between groups. However, on D3, vitamin E levels were found to be significantly higher in serum than in follicular fluid in both groups, whereas malondialdehyde levels were significantly lower in follicular fluid than in serum only in the control group.

CONCLUSION(S)

Before the beginning of ovulation induction, a significant decrease in vitamin E was observed in patients with endometriosis, perhaps because antioxidants are consumed during oxidation reactions. After ovulation induction with exogenous gonadotropins, the group of patients with endometriosis not only presented increased lipid peroxidation but also maintained lower vitamin E levels than the control group, a fact that hypothetically could compromise oocyte quality in endometriotic patients. However, on the day of oocyte retrieval, both serum LP potential and vitamin E levels were found to be similar in the two groups.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Ribeirão Preto Medical School, São Paulo University, Ribeirão Preto, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18249400

Citation

Campos Petean, Carla, et al. "Lipid Peroxidation and Vitamin E in Serum and Follicular Fluid of Infertile Women With Peritoneal Endometriosis Submitted to Controlled Ovarian Hyperstimulation: a Pilot Study." Fertility and Sterility, vol. 90, no. 6, 2008, pp. 2080-5.
Campos Petean C, Ferriani RA, dos Reis RM, et al. Lipid peroxidation and vitamin E in serum and follicular fluid of infertile women with peritoneal endometriosis submitted to controlled ovarian hyperstimulation: a pilot study. Fertil Steril. 2008;90(6):2080-5.
Campos Petean, C., Ferriani, R. A., dos Reis, R. M., de Moura, M. D., Jordão, A. A., & Navarro, P. A. (2008). Lipid peroxidation and vitamin E in serum and follicular fluid of infertile women with peritoneal endometriosis submitted to controlled ovarian hyperstimulation: a pilot study. Fertility and Sterility, 90(6), 2080-5. https://doi.org/10.1016/j.fertnstert.2007.10.072
Campos Petean C, et al. Lipid Peroxidation and Vitamin E in Serum and Follicular Fluid of Infertile Women With Peritoneal Endometriosis Submitted to Controlled Ovarian Hyperstimulation: a Pilot Study. Fertil Steril. 2008;90(6):2080-5. PubMed PMID: 18249400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid peroxidation and vitamin E in serum and follicular fluid of infertile women with peritoneal endometriosis submitted to controlled ovarian hyperstimulation: a pilot study. AU - Campos Petean,Carla, AU - Ferriani,Rui Alberto, AU - dos Reis,Rosana Maria, AU - de Moura,Marcos Dias, AU - Jordão,Alceu Afonso,Jr AU - Navarro,Paula Andrea de Albuquerque Salles, Y1 - 2008/02/04/ PY - 2007/05/30/received PY - 2007/10/27/revised PY - 2007/10/27/accepted PY - 2008/2/6/pubmed PY - 2008/12/24/medline PY - 2008/2/6/entrez SP - 2080 EP - 5 JF - Fertility and sterility JO - Fertil. Steril. VL - 90 IS - 6 N2 - OBJECTIVE: To assess the level of lipid peroxidation (LP) and vitamin E in the follicular fluid and serum of infertile patients, with or without endometriosis, who were submitted to ovulation induction for assisted reproduction procedures. DESIGN: Prospective study. SETTING: Assisted conception unit, university hospital. PATIENT(S): Infertile patients 20 to 38 years of age were selected prospectively and consecutively and were divided into the endometriosis group (17 patients with pelvic endometriosis) and the control group (19 patients with previous tubal ligation or male factor and without endometriosis). INTERVENTION(S): Peripheral blood samples were collected on D1 (before the beginning of the use of gonadotropins), D2 (day of hCG administration), and D3 (day of oocyte retrieval). On D3, follicular-fluid samples free from blood contamination also were collected and stored. MAIN OUTCOME MEASURE(S): Lipid peroxidation was assessed by malondialdehyde quantification by spectrophotometry, and measurement of vitamin E was performed by HLPC. RESULT(S): On D1, no significant difference in LP was observed between groups. However, vitamin E levels were significantly higher in the control group. On D2, LP levels were significantly higher in the endometriosis group compared with in the control group, and vitamin E levels continued to be significantly higher in the control group. On D3, there was no significant difference in serum and follicular-fluid levels of LP and vitamin E between groups. However, on D3, vitamin E levels were found to be significantly higher in serum than in follicular fluid in both groups, whereas malondialdehyde levels were significantly lower in follicular fluid than in serum only in the control group. CONCLUSION(S): Before the beginning of ovulation induction, a significant decrease in vitamin E was observed in patients with endometriosis, perhaps because antioxidants are consumed during oxidation reactions. After ovulation induction with exogenous gonadotropins, the group of patients with endometriosis not only presented increased lipid peroxidation but also maintained lower vitamin E levels than the control group, a fact that hypothetically could compromise oocyte quality in endometriotic patients. However, on the day of oocyte retrieval, both serum LP potential and vitamin E levels were found to be similar in the two groups. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/18249400/Lipid_peroxidation_and_vitamin_E_in_serum_and_follicular_fluid_of_infertile_women_with_peritoneal_endometriosis_submitted_to_controlled_ovarian_hyperstimulation:_a_pilot_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(07)03958-1 DB - PRIME DP - Unbound Medicine ER -