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[Amplification and restriction fragment length polymorphism analysis on the complete sequence of Sta56 gene of Orientia tsutsugamushi isolated from Shandong area].
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Sep; 28(9):886-90.ZL

Abstract

OBJECTIVE

To analyze the genetic differences of Orientia tsutsugamushi (Ot) Sta56 gene between Shandong isolates and other strains deposited in GenBank.

METHODS

PCR and restriction fragment length polymorphism (RFLP) were used to amplify the complete sequence of Ot-Sta56 gene. RFLP profiles of Ot were predicted by a computer program according to their complete sequences of Ot-Sta56 gene. PCR amplicon from XDM2 strain was sequenced and analyzed by Clustal X (1.8) and PHYLIP software.

RESULTS

The complete sequences (about 1.6 kbp) of Ot-Sta56 gene were amplified from B16 strain (isolated from patients), FXS2 strain (isolated from A. agrarius) and XDM2 strain. Four species of restriction endonucleases (Hha I, Hinf I, Hae III, Pst I) were used to digest the PCR amplicons from the 3 isolates. When comparing with the RFLP profiles of prototype Ot, the RFLP profiles of PCR amplicons from the 3 isolates were similar to those of Japan Kawasaki strain, but were quite different from the international reference strains Gilliam, Karp, Kato. Results from DNA sequence analysis showed that the complete sequence of Ot-Sta56 gene homology to Japan Kawasaki strain of XDM2 strain was 97%, and deduced amino acid sequence was 92%.

CONCLUSION

Data from the complete sequence of Sta56 gene indicated that the genotypes of Ot isolates in Shandong province were similar, but with distinction from the Kawasaki strain.

Authors+Show Affiliations

Liu YX
General Hospital of PLA, Beijing 100853, China.
Zhang Q
No affiliation info available
Zhao ZT
No affiliation info available
Yang ZQ
No affiliation info available
Yang LP
No affiliation info available
Zhang PH
No affiliation info available
Yang H
No affiliation info available
Yuan YE
No affiliation info available
Wei H
No affiliation info available
Suo JJ
No affiliation info available
Xing YB
No affiliation info available
Jia N
No affiliation info available
Gao Y
No affiliation info available
Cao WC
No affiliation info available

MeSH

Amplified Fragment Length Polymorphism AnalysisAntigens, BacterialBacterial ProteinsBacterial Typing TechniquesDNA, BacterialGenes, BacterialMembrane ProteinsOrientia tsutsugamushiPolymorphism, Restriction Fragment LengthSequence Analysis, DNA

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18251273

Citation

Liu, Yun-Xi, et al. "[Amplification and Restriction Fragment Length Polymorphism Analysis On the Complete Sequence of Sta56 Gene of Orientia Tsutsugamushi Isolated From Shandong Area]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 28, no. 9, 2007, pp. 886-90.
Liu YX, Zhang Q, Zhao ZT, et al. [Amplification and restriction fragment length polymorphism analysis on the complete sequence of Sta56 gene of Orientia tsutsugamushi isolated from Shandong area]. Zhonghua Liu Xing Bing Xue Za Zhi. 2007;28(9):886-90.
Liu, Y. X., Zhang, Q., Zhao, Z. T., Yang, Z. Q., Yang, L. P., Zhang, P. H., Yang, H., Yuan, Y. E., Wei, H., Suo, J. J., Xing, Y. B., Jia, N., Gao, Y., & Cao, W. C. (2007). [Amplification and restriction fragment length polymorphism analysis on the complete sequence of Sta56 gene of Orientia tsutsugamushi isolated from Shandong area]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 28(9), 886-90.
Liu YX, et al. [Amplification and Restriction Fragment Length Polymorphism Analysis On the Complete Sequence of Sta56 Gene of Orientia Tsutsugamushi Isolated From Shandong Area]. Zhonghua Liu Xing Bing Xue Za Zhi. 2007;28(9):886-90. PubMed PMID: 18251273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Amplification and restriction fragment length polymorphism analysis on the complete sequence of Sta56 gene of Orientia tsutsugamushi isolated from Shandong area]. AU - Liu,Yun-Xi, AU - Zhang,Qian, AU - Zhao,Zhong-Tang, AU - Yang,Zhan-Qing, AU - Yang,Li-Ping, AU - Zhang,Pan-He, AU - Yang,Hong, AU - Yuan,Yun-E, AU - Wei,Hua, AU - Suo,Ji-Jiang, AU - Xing,Yu-Bin, AU - Jia,Ning, AU - Gao,Yan, AU - Cao,Wu-Chun, PY - 2008/2/7/pubmed PY - 2010/5/14/medline PY - 2008/2/7/entrez SP - 886 EP - 90 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 28 IS - 9 N2 - OBJECTIVE: To analyze the genetic differences of Orientia tsutsugamushi (Ot) Sta56 gene between Shandong isolates and other strains deposited in GenBank. METHODS: PCR and restriction fragment length polymorphism (RFLP) were used to amplify the complete sequence of Ot-Sta56 gene. RFLP profiles of Ot were predicted by a computer program according to their complete sequences of Ot-Sta56 gene. PCR amplicon from XDM2 strain was sequenced and analyzed by Clustal X (1.8) and PHYLIP software. RESULTS: The complete sequences (about 1.6 kbp) of Ot-Sta56 gene were amplified from B16 strain (isolated from patients), FXS2 strain (isolated from A. agrarius) and XDM2 strain. Four species of restriction endonucleases (Hha I, Hinf I, Hae III, Pst I) were used to digest the PCR amplicons from the 3 isolates. When comparing with the RFLP profiles of prototype Ot, the RFLP profiles of PCR amplicons from the 3 isolates were similar to those of Japan Kawasaki strain, but were quite different from the international reference strains Gilliam, Karp, Kato. Results from DNA sequence analysis showed that the complete sequence of Ot-Sta56 gene homology to Japan Kawasaki strain of XDM2 strain was 97%, and deduced amino acid sequence was 92%. CONCLUSION: Data from the complete sequence of Sta56 gene indicated that the genotypes of Ot isolates in Shandong province were similar, but with distinction from the Kawasaki strain. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/18251273/[Amplification_and_restriction_fragment_length_polymorphism_analysis_on_the_complete_sequence_of_Sta56_gene_of_Orientia_tsutsugamushi_isolated_from_Shandong_area]_ DB - PRIME DP - Unbound Medicine ER -