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Root cementum may modulate gene expression during periodontal regeneration: a preliminary study in humans.
J Periodontol 2008; 79(2):323-31JP

Abstract

BACKGROUND

Previous data demonstrated that root cementum may affect periodontal regeneration. As such, this study aimed to explore further possible mechanisms involved in this process by investigating in humans whether root cementum modulates gene expression in the regenerating tissue formed under membrane-protected intrabony defects.

METHODS

Thirty subjects with deep intrabony defects (> or =5 mm; 2- or 3-wall) were selected and assigned to the control or test group. The control group received scaling and root planing with the removal of granulation tissue and root cementum; the test group underwent removal of granulation tissue and soft microbial deposits by cleaning the root surface with a microbrush and saline solution, aiming at cementum preservation. Guided tissue regeneration (GTR) was applied to both groups. Twenty-one days later, the newly formed tissue under the membrane was assessed for the expression of the following genes: alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), platelet-derived growth factor-alpha (PDGFA), bone sialoprotein (BSP), and basic fibroblast growth factor (bFGF).

RESULTS

Data analysis demonstrated that mRNA levels for PDGFA, BSP, and bFGF were higher in the sites where root cementum was kept in place compared to the sites where root cementum was removed completely as part of the periodontal therapy (P <0.05); in contrast, OCN levels were lower (P <0.05). No difference for ALP or OPN was observed between the control and test groups (P >0.05).

CONCLUSION

Root cementum may modulate the expression of growth and mineral-associated factors during periodontal regeneration.

Authors+Show Affiliations

Department of Dentistry, Division of Periodontics, School of Biologic and Health Sciences, Federal University of Vales of Jequitinhonha and Mucuri, Diamantina, MG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18251647

Citation

Gonçalves, Patricia F., et al. "Root Cementum May Modulate Gene Expression During Periodontal Regeneration: a Preliminary Study in Humans." Journal of Periodontology, vol. 79, no. 2, 2008, pp. 323-31.
Gonçalves PF, Lima LL, Sallum EA, et al. Root cementum may modulate gene expression during periodontal regeneration: a preliminary study in humans. J Periodontol. 2008;79(2):323-31.
Gonçalves, P. F., Lima, L. L., Sallum, E. A., Casati, M. Z., & Nociti, F. H. (2008). Root cementum may modulate gene expression during periodontal regeneration: a preliminary study in humans. Journal of Periodontology, 79(2), pp. 323-31. doi:10.1902/jop.2008.070327.
Gonçalves PF, et al. Root Cementum May Modulate Gene Expression During Periodontal Regeneration: a Preliminary Study in Humans. J Periodontol. 2008;79(2):323-31. PubMed PMID: 18251647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Root cementum may modulate gene expression during periodontal regeneration: a preliminary study in humans. AU - Gonçalves,Patricia F, AU - Lima,Liana L, AU - Sallum,Enilson A, AU - Casati,Márcio Z, AU - Nociti,Francisco H,Jr PY - 2008/2/7/pubmed PY - 2008/3/19/medline PY - 2008/2/7/entrez SP - 323 EP - 31 JF - Journal of periodontology JO - J. Periodontol. VL - 79 IS - 2 N2 - BACKGROUND: Previous data demonstrated that root cementum may affect periodontal regeneration. As such, this study aimed to explore further possible mechanisms involved in this process by investigating in humans whether root cementum modulates gene expression in the regenerating tissue formed under membrane-protected intrabony defects. METHODS: Thirty subjects with deep intrabony defects (> or =5 mm; 2- or 3-wall) were selected and assigned to the control or test group. The control group received scaling and root planing with the removal of granulation tissue and root cementum; the test group underwent removal of granulation tissue and soft microbial deposits by cleaning the root surface with a microbrush and saline solution, aiming at cementum preservation. Guided tissue regeneration (GTR) was applied to both groups. Twenty-one days later, the newly formed tissue under the membrane was assessed for the expression of the following genes: alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), platelet-derived growth factor-alpha (PDGFA), bone sialoprotein (BSP), and basic fibroblast growth factor (bFGF). RESULTS: Data analysis demonstrated that mRNA levels for PDGFA, BSP, and bFGF were higher in the sites where root cementum was kept in place compared to the sites where root cementum was removed completely as part of the periodontal therapy (P <0.05); in contrast, OCN levels were lower (P <0.05). No difference for ALP or OPN was observed between the control and test groups (P >0.05). CONCLUSION: Root cementum may modulate the expression of growth and mineral-associated factors during periodontal regeneration. SN - 0022-3492 UR - https://www.unboundmedicine.com/medline/citation/18251647/Root_cementum_may_modulate_gene_expression_during_periodontal_regeneration:_a_preliminary_study_in_humans_ L2 - https://doi.org/10.1902/jop.2008.070327 DB - PRIME DP - Unbound Medicine ER -