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Effect of phytocompounds from the heartwood of Acacia confusa on inflammatory mediator production.
J Agric Food Chem. 2008 Mar 12; 56(5):1567-73.JA

Abstract

Acacia confusa Merr. (Leguminosae) is traditionally used as a medicinal plant in Taiwan. In the present study, anti-inflammatory activity of extracts from the heartwood of A. confusa were investigated for the first time. Results demonstrated that ethanolic extracts of A. confusa heartwood strongly suppressed NO production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Among all fractions derived from ethanolic extracts, the EtOAc fraction exhibited the best inhibitory activity. Following column chromatography and reverse-phase high-performance liquid chromatography, 13 specific phytocompounds including 5 new flavonoids (i.e., 7,8,3',4'-tetrahydroxy-4-methoxyflavan-3-ol, 7,8,3',4'-tetrahydroxyflavone, 7,8,3'-trihydroxy-3,4'-dimethoxyflavone, 7,3',4'-trihydroxyflavone, and 7,3',4'-trihydroxy-3-methoxyflavone) were isolated and identified from the EtOAc fraction. In addition, melanoxetin (3,7,8,3',4'-pentahydroxyflavone), a major compound in the EtOAc fraction, markedly suppressed LPS-induced NO and prostaglandin E 2 (PGE 2) production. Moreover, melanoxetin completely suppressed gene expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) at 50 and 100 microM, respectively. This is the first report to identify the inhibitory bioactivities of melanoxetin on iNOS and COX-2.

Authors+Show Affiliations

Department of Forestry, National Chung-Hsing University, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18254591

Citation

Wu, Jyh-Horng, et al. "Effect of Phytocompounds From the Heartwood of Acacia Confusa On Inflammatory Mediator Production." Journal of Agricultural and Food Chemistry, vol. 56, no. 5, 2008, pp. 1567-73.
Wu JH, Tung YT, Chien SC, et al. Effect of phytocompounds from the heartwood of Acacia confusa on inflammatory mediator production. J Agric Food Chem. 2008;56(5):1567-73.
Wu, J. H., Tung, Y. T., Chien, S. C., Wang, S. Y., Kuo, Y. H., Shyur, L. F., & Chang, S. T. (2008). Effect of phytocompounds from the heartwood of Acacia confusa on inflammatory mediator production. Journal of Agricultural and Food Chemistry, 56(5), 1567-73. https://doi.org/10.1021/jf072922s
Wu JH, et al. Effect of Phytocompounds From the Heartwood of Acacia Confusa On Inflammatory Mediator Production. J Agric Food Chem. 2008 Mar 12;56(5):1567-73. PubMed PMID: 18254591.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of phytocompounds from the heartwood of Acacia confusa on inflammatory mediator production. AU - Wu,Jyh-Horng, AU - Tung,Yu-Tang, AU - Chien,Shih-Chang, AU - Wang,Sheng-Yang, AU - Kuo,Yueh-Hsiung, AU - Shyur,Lie-Fen, AU - Chang,Shang-Tzen, Y1 - 2008/02/07/ PY - 2008/2/8/pubmed PY - 2008/4/29/medline PY - 2008/2/8/entrez SP - 1567 EP - 73 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 56 IS - 5 N2 - Acacia confusa Merr. (Leguminosae) is traditionally used as a medicinal plant in Taiwan. In the present study, anti-inflammatory activity of extracts from the heartwood of A. confusa were investigated for the first time. Results demonstrated that ethanolic extracts of A. confusa heartwood strongly suppressed NO production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Among all fractions derived from ethanolic extracts, the EtOAc fraction exhibited the best inhibitory activity. Following column chromatography and reverse-phase high-performance liquid chromatography, 13 specific phytocompounds including 5 new flavonoids (i.e., 7,8,3',4'-tetrahydroxy-4-methoxyflavan-3-ol, 7,8,3',4'-tetrahydroxyflavone, 7,8,3'-trihydroxy-3,4'-dimethoxyflavone, 7,3',4'-trihydroxyflavone, and 7,3',4'-trihydroxy-3-methoxyflavone) were isolated and identified from the EtOAc fraction. In addition, melanoxetin (3,7,8,3',4'-pentahydroxyflavone), a major compound in the EtOAc fraction, markedly suppressed LPS-induced NO and prostaglandin E 2 (PGE 2) production. Moreover, melanoxetin completely suppressed gene expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) at 50 and 100 microM, respectively. This is the first report to identify the inhibitory bioactivities of melanoxetin on iNOS and COX-2. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/18254591/Effect_of_phytocompounds_from_the_heartwood_of_Acacia_confusa_on_inflammatory_mediator_production_ L2 - https://dx.doi.org/10.1021/jf072922s DB - PRIME DP - Unbound Medicine ER -