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Selenium speciation and bioavailability in biofortified products using species-unspecific isotope dilution and reverse phase ion pairing-inductively coupled plasma-mass spectrometry.
J Agric Food Chem. 2008 Mar 12; 56(5):1772-9.JA

Abstract

In some regions of the world, where the bioavailability of selenium (Se) in soil is low and/or declining (e.g., due to use of high-sulfur fertilizers), there is increased risk of adverse affects on animals and human health. In recent years, increased research attention has focused on understanding the relationships between Se contents in foods and supplements and their nutritional benefits for animal and humans. The objective of this study was to use a species-unspecific isotope dilution and reverse phase ion pairing-inductively coupled plasma-mass spectrometry techniques for the identification and quantification of Se species in biofortified grains (i.e., wheat and triticale), flour, and wheat biscuits. The information on Se species was used to gain an understanding of the bioavailability of Se in biofortified and process-fortified wheat biscuits used in a clinical trail. The major Se species identified in biofortified and process-fortified samples were selenomethionine (76-85%) and selenomethionine selenoxide (51-60%), respectively. Total plasma Se concentrations in the biofortified Se exposure group were found to increase throughout the 6 month trial period (mean=122 microg L(-1) at 0 months to 194 microg L(-1) at 6 months). In contrast, the trial group exposed to process-fortified Se biscuits showed little increase in mean total Se plasma concentrations until 4 months of exposure (mean=122 microg L(-1) at 0 months to 140 microg L(-1) at 4 months) that remained constant until the end of the trial period (mean=140 microg L(-1) at 4 months to 138 microg L(-1) at 6 months). The difference in total Se plasma concentrations may be due to the presence and bioavailability of different Se species in biofortified and process-fortified biscuits. An understanding of Se speciation in foods enables better understanding of pathways and their potential benefits for animals and humans.

Authors+Show Affiliations

Centre for Environmental Contaminant Research, CSIRO, Waite Road, Urrbrae, South Australia 5064, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18254593

Citation

Kirby, J K., et al. "Selenium Speciation and Bioavailability in Biofortified Products Using Species-unspecific Isotope Dilution and Reverse Phase Ion Pairing-inductively Coupled Plasma-mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 56, no. 5, 2008, pp. 1772-9.
Kirby JK, Lyons GH, Karkkainen MP. Selenium speciation and bioavailability in biofortified products using species-unspecific isotope dilution and reverse phase ion pairing-inductively coupled plasma-mass spectrometry. J Agric Food Chem. 2008;56(5):1772-9.
Kirby, J. K., Lyons, G. H., & Karkkainen, M. P. (2008). Selenium speciation and bioavailability in biofortified products using species-unspecific isotope dilution and reverse phase ion pairing-inductively coupled plasma-mass spectrometry. Journal of Agricultural and Food Chemistry, 56(5), 1772-9. https://doi.org/10.1021/jf073030v
Kirby JK, Lyons GH, Karkkainen MP. Selenium Speciation and Bioavailability in Biofortified Products Using Species-unspecific Isotope Dilution and Reverse Phase Ion Pairing-inductively Coupled Plasma-mass Spectrometry. J Agric Food Chem. 2008 Mar 12;56(5):1772-9. PubMed PMID: 18254593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium speciation and bioavailability in biofortified products using species-unspecific isotope dilution and reverse phase ion pairing-inductively coupled plasma-mass spectrometry. AU - Kirby,J K, AU - Lyons,G H, AU - Karkkainen,M P, Y1 - 2008/02/07/ PY - 2008/2/8/pubmed PY - 2008/4/29/medline PY - 2008/2/8/entrez SP - 1772 EP - 9 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 56 IS - 5 N2 - In some regions of the world, where the bioavailability of selenium (Se) in soil is low and/or declining (e.g., due to use of high-sulfur fertilizers), there is increased risk of adverse affects on animals and human health. In recent years, increased research attention has focused on understanding the relationships between Se contents in foods and supplements and their nutritional benefits for animal and humans. The objective of this study was to use a species-unspecific isotope dilution and reverse phase ion pairing-inductively coupled plasma-mass spectrometry techniques for the identification and quantification of Se species in biofortified grains (i.e., wheat and triticale), flour, and wheat biscuits. The information on Se species was used to gain an understanding of the bioavailability of Se in biofortified and process-fortified wheat biscuits used in a clinical trail. The major Se species identified in biofortified and process-fortified samples were selenomethionine (76-85%) and selenomethionine selenoxide (51-60%), respectively. Total plasma Se concentrations in the biofortified Se exposure group were found to increase throughout the 6 month trial period (mean=122 microg L(-1) at 0 months to 194 microg L(-1) at 6 months). In contrast, the trial group exposed to process-fortified Se biscuits showed little increase in mean total Se plasma concentrations until 4 months of exposure (mean=122 microg L(-1) at 0 months to 140 microg L(-1) at 4 months) that remained constant until the end of the trial period (mean=140 microg L(-1) at 4 months to 138 microg L(-1) at 6 months). The difference in total Se plasma concentrations may be due to the presence and bioavailability of different Se species in biofortified and process-fortified biscuits. An understanding of Se speciation in foods enables better understanding of pathways and their potential benefits for animals and humans. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/18254593/Selenium_speciation_and_bioavailability_in_biofortified_products_using_species_unspecific_isotope_dilution_and_reverse_phase_ion_pairing_inductively_coupled_plasma_mass_spectrometry_ L2 - https://dx.doi.org/10.1021/jf073030v DB - PRIME DP - Unbound Medicine ER -