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Simultaneous determination of berberine, palmatine and jatrorrhizine by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of coptis-evodia herb couple.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 01; 863(2):195-205.JC

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine berberine, palmatine and jatrorrhizine in rat plasma. After mixing with the internal standard (IS) tetrahydropalmatine, plasma samples were pretreated by protein precipitation with acetonitrile-methanol (1:2, v/v). Chromatographic separation was carried out on a C18 column using a mixture of water (containing 0.1% formic acid) and acetonitrile (30:70, v/v) as mobile phase. The detection was performed by selected reaction monitoring (SRM) mode via electrospray ionization (ESI) source operating in the positive ionization mode. The method was linear over the concentration range of 1.0-250.0 ng/mL for all components. The intra- and inter-day precision values were less than 14.6% and the deviations were within +/-4.0%. The fully validated LC-MS/MS method has been successfully applied to the pharmacokinetic study of berberine, palmatine and jatrorrhizine in rat plasma after oral administration of coptis-evodia herb couple. Three peaks were observed in both individual and mean plasma-concentration curves of berberine, palmatine and jatrorrhizine, which may be attributed to distribution re-absorption and enterohepatic circulation.

Authors+Show Affiliations

Laboratory of Pharmaceutical Analysis & Quality Assessment, School of Pharmaceutical Sciences, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

18258496

Citation

Deng, Yating, et al. "Simultaneous Determination of Berberine, Palmatine and Jatrorrhizine By Liquid Chromatography-tandem Mass Spectrometry in Rat Plasma and Its Application in a Pharmacokinetic Study After Oral Administration of Coptis-evodia Herb Couple." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 863, no. 2, 2008, pp. 195-205.
Deng Y, Liao Q, Li S, et al. Simultaneous determination of berberine, palmatine and jatrorrhizine by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of coptis-evodia herb couple. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;863(2):195-205.
Deng, Y., Liao, Q., Li, S., Bi, K., Pan, B., & Xie, Z. (2008). Simultaneous determination of berberine, palmatine and jatrorrhizine by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of coptis-evodia herb couple. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 863(2), 195-205. https://doi.org/10.1016/j.jchromb.2007.12.028
Deng Y, et al. Simultaneous Determination of Berberine, Palmatine and Jatrorrhizine By Liquid Chromatography-tandem Mass Spectrometry in Rat Plasma and Its Application in a Pharmacokinetic Study After Oral Administration of Coptis-evodia Herb Couple. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 1;863(2):195-205. PubMed PMID: 18258496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of berberine, palmatine and jatrorrhizine by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of coptis-evodia herb couple. AU - Deng,Yating, AU - Liao,Qiongfeng, AU - Li,Suhua, AU - Bi,Kaishun, AU - Pan,Biyan, AU - Xie,Zhiyong, Y1 - 2008/01/21/ PY - 2007/08/28/received PY - 2007/11/27/revised PY - 2007/12/24/accepted PY - 2008/2/9/pubmed PY - 2008/7/11/medline PY - 2008/2/9/entrez SP - 195 EP - 205 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 863 IS - 2 N2 - A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine berberine, palmatine and jatrorrhizine in rat plasma. After mixing with the internal standard (IS) tetrahydropalmatine, plasma samples were pretreated by protein precipitation with acetonitrile-methanol (1:2, v/v). Chromatographic separation was carried out on a C18 column using a mixture of water (containing 0.1% formic acid) and acetonitrile (30:70, v/v) as mobile phase. The detection was performed by selected reaction monitoring (SRM) mode via electrospray ionization (ESI) source operating in the positive ionization mode. The method was linear over the concentration range of 1.0-250.0 ng/mL for all components. The intra- and inter-day precision values were less than 14.6% and the deviations were within +/-4.0%. The fully validated LC-MS/MS method has been successfully applied to the pharmacokinetic study of berberine, palmatine and jatrorrhizine in rat plasma after oral administration of coptis-evodia herb couple. Three peaks were observed in both individual and mean plasma-concentration curves of berberine, palmatine and jatrorrhizine, which may be attributed to distribution re-absorption and enterohepatic circulation. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18258496/Simultaneous_determination_of_berberine_palmatine_and_jatrorrhizine_by_liquid_chromatography_tandem_mass_spectrometry_in_rat_plasma_and_its_application_in_a_pharmacokinetic_study_after_oral_administration_of_coptis_evodia_herb_couple_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00010-X DB - PRIME DP - Unbound Medicine ER -