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A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides.
J Chromatogr A. 2008 Mar 28; 1185(2):251-7.JC

Abstract

We have developed a reversed-phase high-performance liquid chromatography pulsed amperometric detection (RP-HPLC-PAD) method for the determination of glycosides. It is sensitive, repeatable, and selective without the pretreatment step. Ginsenosides were separated completely in 50 min using an water-acetonitrile gradient as the eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limit (S/N=3) was 0.02-0.07 ng and the quantification limit (S/N=10) was 0.1-0.2 ng. The coefficient of linear regression was 0.9984-0.9998 for concentrations between 1 and 50 microg/mL. The intra- and inter-day precision (RSD) was less than 6.35% in Ginseng Radix and Shy-jiun-tzyy-tang extracts. The average recoveries from Ginseng Radix and Shy-jiun-tzyy-tang extracts were 98.19-105.45% and 96.89-102.22%, respectively.

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Authors+Show Affiliations

Kwon HJ
Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
Jeong JS
No affiliation info available
Lee YM
No affiliation info available
Hong SP
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDrugs, Chinese HerbalGinsenosidesPanaxSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18262196

Citation

Kwon, Ha-Jeong, et al. "A Reversed-phase High-performance Liquid Chromatography Method With Pulsed Amperometric Detection for the Determination of Glycosides." Journal of Chromatography. A, vol. 1185, no. 2, 2008, pp. 251-7.
Kwon HJ, Jeong JS, Lee YM, et al. A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides. J Chromatogr A. 2008;1185(2):251-7.
Kwon, H. J., Jeong, J. S., Lee, Y. M., & Hong, S. P. (2008). A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides. Journal of Chromatography. A, 1185(2), 251-7. https://doi.org/10.1016/j.chroma.2008.01.052
Kwon HJ, et al. A Reversed-phase High-performance Liquid Chromatography Method With Pulsed Amperometric Detection for the Determination of Glycosides. J Chromatogr A. 2008 Mar 28;1185(2):251-7. PubMed PMID: 18262196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides. AU - Kwon,Ha-Jeong, AU - Jeong,Ji-Seon, AU - Lee,Yong-Moon, AU - Hong,Seon-Pyo, Y1 - 2008/01/31/ PY - 2007/11/20/received PY - 2008/01/15/revised PY - 2008/01/25/accepted PY - 2008/2/12/pubmed PY - 2008/5/31/medline PY - 2008/2/12/entrez SP - 251 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1185 IS - 2 N2 - We have developed a reversed-phase high-performance liquid chromatography pulsed amperometric detection (RP-HPLC-PAD) method for the determination of glycosides. It is sensitive, repeatable, and selective without the pretreatment step. Ginsenosides were separated completely in 50 min using an water-acetonitrile gradient as the eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limit (S/N=3) was 0.02-0.07 ng and the quantification limit (S/N=10) was 0.1-0.2 ng. The coefficient of linear regression was 0.9984-0.9998 for concentrations between 1 and 50 microg/mL. The intra- and inter-day precision (RSD) was less than 6.35% in Ginseng Radix and Shy-jiun-tzyy-tang extracts. The average recoveries from Ginseng Radix and Shy-jiun-tzyy-tang extracts were 98.19-105.45% and 96.89-102.22%, respectively. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18262196/A_reversed_phase_high_performance_liquid_chromatography_method_with_pulsed_amperometric_detection_for_the_determination_of_glycosides_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00172-6 DB - PRIME DP - Unbound Medicine ER -