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A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides.
J Chromatogr A. 2008 Mar 28; 1185(2):251-7.JC

Abstract

We have developed a reversed-phase high-performance liquid chromatography pulsed amperometric detection (RP-HPLC-PAD) method for the determination of glycosides. It is sensitive, repeatable, and selective without the pretreatment step. Ginsenosides were separated completely in 50 min using an water-acetonitrile gradient as the eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limit (S/N=3) was 0.02-0.07 ng and the quantification limit (S/N=10) was 0.1-0.2 ng. The coefficient of linear regression was 0.9984-0.9998 for concentrations between 1 and 50 microg/mL. The intra- and inter-day precision (RSD) was less than 6.35% in Ginseng Radix and Shy-jiun-tzyy-tang extracts. The average recoveries from Ginseng Radix and Shy-jiun-tzyy-tang extracts were 98.19-105.45% and 96.89-102.22%, respectively.

Authors+Show Affiliations

Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18262196

Citation

Kwon, Ha-Jeong, et al. "A Reversed-phase High-performance Liquid Chromatography Method With Pulsed Amperometric Detection for the Determination of Glycosides." Journal of Chromatography. A, vol. 1185, no. 2, 2008, pp. 251-7.
Kwon HJ, Jeong JS, Lee YM, et al. A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides. J Chromatogr A. 2008;1185(2):251-7.
Kwon, H. J., Jeong, J. S., Lee, Y. M., & Hong, S. P. (2008). A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides. Journal of Chromatography. A, 1185(2), 251-7. https://doi.org/10.1016/j.chroma.2008.01.052
Kwon HJ, et al. A Reversed-phase High-performance Liquid Chromatography Method With Pulsed Amperometric Detection for the Determination of Glycosides. J Chromatogr A. 2008 Mar 28;1185(2):251-7. PubMed PMID: 18262196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides. AU - Kwon,Ha-Jeong, AU - Jeong,Ji-Seon, AU - Lee,Yong-Moon, AU - Hong,Seon-Pyo, Y1 - 2008/01/31/ PY - 2007/11/20/received PY - 2008/01/15/revised PY - 2008/01/25/accepted PY - 2008/2/12/pubmed PY - 2008/5/31/medline PY - 2008/2/12/entrez SP - 251 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1185 IS - 2 N2 - We have developed a reversed-phase high-performance liquid chromatography pulsed amperometric detection (RP-HPLC-PAD) method for the determination of glycosides. It is sensitive, repeatable, and selective without the pretreatment step. Ginsenosides were separated completely in 50 min using an water-acetonitrile gradient as the eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limit (S/N=3) was 0.02-0.07 ng and the quantification limit (S/N=10) was 0.1-0.2 ng. The coefficient of linear regression was 0.9984-0.9998 for concentrations between 1 and 50 microg/mL. The intra- and inter-day precision (RSD) was less than 6.35% in Ginseng Radix and Shy-jiun-tzyy-tang extracts. The average recoveries from Ginseng Radix and Shy-jiun-tzyy-tang extracts were 98.19-105.45% and 96.89-102.22%, respectively. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18262196/A_reversed_phase_high_performance_liquid_chromatography_method_with_pulsed_amperometric_detection_for_the_determination_of_glycosides_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00172-6 DB - PRIME DP - Unbound Medicine ER -