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Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity.
Lett Appl Microbiol. 2008 Mar; 46(3):371-6.LA

Abstract

AIMS

The aim of this work was to analyse the antimicrobial properties of a purified lectin from Eugenia uniflora L. seeds.

METHODS AND RESULTS

The E. uniflora lectin (EuniSL) was isolated from the seed extract and purified by ion-exchange chromatography in DEAE-Sephadex with a purification factor of 11.68. The purified lectin showed a single band on denaturing electrophoresis, with a molecular mass of 67 kDa. EuniSL agglutinated rabbit and human erythrocytes with a higher specificity for rabbit erythrocytes. The haemagglutination was not inhibited by the tested carbohydrates but glycoproteins exerted a strong inhibitory action. The lectin proved to be thermo resistant with the highest stability at pH 6.5 and divalent ions did not affect its activity. EuniSL demonstrated a remarkable nonselective antibacterial activity. EuniSL strongly inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella sp. with a minimum inhibitory concentration (MIC) of 1.5 microg ml(-1), and moderately inhibited the growth of Bacillus subtilis, Streptococcus sp. and Escherichia coli with a MIC of 16.5 microg ml(-1).

CONCLUSIONS

EuniSL was found to be effective against bacteria.

SIGNIFICANCE AND IMPACT OF THE STUDY

The strong antibacterial activity of the studied lectin indicates a high potential for clinical microbiology and therapeutic applications.

Authors+Show Affiliations

Departamento de Bioquímica - Laboratório de Glicoproteínas, Universidade Federal de Pernambuco, Recife, PE, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18266644

Citation

Oliveira, M D L., et al. "Purification of a Lectin From Eugenia Uniflora L. Seeds and Its Potential Antibacterial Activity." Letters in Applied Microbiology, vol. 46, no. 3, 2008, pp. 371-6.
Oliveira MD, Andrade CA, Santos-Magalhães NS, et al. Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity. Lett Appl Microbiol. 2008;46(3):371-6.
Oliveira, M. D., Andrade, C. A., Santos-Magalhães, N. S., Coelho, L. C., Teixeira, J. A., Carneiro-da-Cunha, M. G., & Correia, M. T. (2008). Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity. Letters in Applied Microbiology, 46(3), 371-6. https://doi.org/10.1111/j.1472-765X.2007.02319.x
Oliveira MD, et al. Purification of a Lectin From Eugenia Uniflora L. Seeds and Its Potential Antibacterial Activity. Lett Appl Microbiol. 2008;46(3):371-6. PubMed PMID: 18266644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification of a lectin from Eugenia uniflora L. seeds and its potential antibacterial activity. AU - Oliveira,M D L, AU - Andrade,C A S, AU - Santos-Magalhães,N S, AU - Coelho,L C B B, AU - Teixeira,J A, AU - Carneiro-da-Cunha,M G, AU - Correia,M T S, Y1 - 2008/02/07/ PY - 2008/2/13/pubmed PY - 2008/3/25/medline PY - 2008/2/13/entrez SP - 371 EP - 6 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 46 IS - 3 N2 - AIMS: The aim of this work was to analyse the antimicrobial properties of a purified lectin from Eugenia uniflora L. seeds. METHODS AND RESULTS: The E. uniflora lectin (EuniSL) was isolated from the seed extract and purified by ion-exchange chromatography in DEAE-Sephadex with a purification factor of 11.68. The purified lectin showed a single band on denaturing electrophoresis, with a molecular mass of 67 kDa. EuniSL agglutinated rabbit and human erythrocytes with a higher specificity for rabbit erythrocytes. The haemagglutination was not inhibited by the tested carbohydrates but glycoproteins exerted a strong inhibitory action. The lectin proved to be thermo resistant with the highest stability at pH 6.5 and divalent ions did not affect its activity. EuniSL demonstrated a remarkable nonselective antibacterial activity. EuniSL strongly inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella sp. with a minimum inhibitory concentration (MIC) of 1.5 microg ml(-1), and moderately inhibited the growth of Bacillus subtilis, Streptococcus sp. and Escherichia coli with a MIC of 16.5 microg ml(-1). CONCLUSIONS: EuniSL was found to be effective against bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY: The strong antibacterial activity of the studied lectin indicates a high potential for clinical microbiology and therapeutic applications. SN - 1472-765X UR - https://www.unboundmedicine.com/medline/citation/18266644/Purification_of_a_lectin_from_Eugenia_uniflora_L__seeds_and_its_potential_antibacterial_activity_ L2 - https://doi.org/10.1111/j.1472-765X.2007.02319.x DB - PRIME DP - Unbound Medicine ER -