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The tryptophan pathway is involved in the defense responses of rice against pathogenic infection via serotonin production.
Plant J. 2008 May; 54(3):481-95.PJ

Abstract

The upregulation of the tryptophan (Trp) pathway in rice leaves infected by Bipolaris oryzae was indicated by: (i) enhanced enzyme activity of anthranilate synthase (AS), which regulates metabolic flux in the Trp pathway; (ii) elevated levels of the AS (OASA2, OASB1, and OASB2) transcripts; and (iii) increases in the contents of anthranilate, indole, and Trp. The measurement of the contents of Trp-derived metabolites by high-performance liquid chromatography coupled with tandem mass spectrometry revealed that serotonin and its hydroxycinnamic acid amides were accumulated in infected leaves. Serotonin accumulation was preceded by a transient increase in the tryptamine content and by marked activation of Trp decarboxylase, indicating that enhanced Trp production is linked to the formation of serotonin from Trp via tryptamine. Feeding of radiolabeled serotonin to inoculated leaves demonstrated that serotonin is incorporated into the cell walls of lesion tissue. The leaves of a propagating-type lesion mimic mutant (sl, Sekiguchi lesion) lacked both serotonin production and deposition of unextractable brown material at the infection sites, and showed increased susceptibility to B. oryzae infection. Treating the mutant with serotonin restored deposition of brown material at the lesion site. In addition, the serotonin treatment suppressed the growth of fungal hyphae in the leaf tissues of the sl mutant. These findings indicated that the activation of the Trp pathway is involved in the establishment of effective physical defenses by producing serotonin in rice leaves.

Authors+Show Affiliations

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18266919

Citation

Ishihara, Atsushi, et al. "The Tryptophan Pathway Is Involved in the Defense Responses of Rice Against Pathogenic Infection Via Serotonin Production." The Plant Journal : for Cell and Molecular Biology, vol. 54, no. 3, 2008, pp. 481-95.
Ishihara A, Hashimoto Y, Tanaka C, et al. The tryptophan pathway is involved in the defense responses of rice against pathogenic infection via serotonin production. Plant J. 2008;54(3):481-95.
Ishihara, A., Hashimoto, Y., Tanaka, C., Dubouzet, J. G., Nakao, T., Matsuda, F., Nishioka, T., Miyagawa, H., & Wakasa, K. (2008). The tryptophan pathway is involved in the defense responses of rice against pathogenic infection via serotonin production. The Plant Journal : for Cell and Molecular Biology, 54(3), 481-95. https://doi.org/10.1111/j.1365-313X.2008.03441.x
Ishihara A, et al. The Tryptophan Pathway Is Involved in the Defense Responses of Rice Against Pathogenic Infection Via Serotonin Production. Plant J. 2008;54(3):481-95. PubMed PMID: 18266919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The tryptophan pathway is involved in the defense responses of rice against pathogenic infection via serotonin production. AU - Ishihara,Atsushi, AU - Hashimoto,Yumi, AU - Tanaka,Chihiro, AU - Dubouzet,Joseph G, AU - Nakao,Takahito, AU - Matsuda,Fumio, AU - Nishioka,Takaaki, AU - Miyagawa,Hisashi, AU - Wakasa,Kyo, Y1 - 2008/02/07/ PY - 2008/2/13/pubmed PY - 2008/8/8/medline PY - 2008/2/13/entrez SP - 481 EP - 95 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 54 IS - 3 N2 - The upregulation of the tryptophan (Trp) pathway in rice leaves infected by Bipolaris oryzae was indicated by: (i) enhanced enzyme activity of anthranilate synthase (AS), which regulates metabolic flux in the Trp pathway; (ii) elevated levels of the AS (OASA2, OASB1, and OASB2) transcripts; and (iii) increases in the contents of anthranilate, indole, and Trp. The measurement of the contents of Trp-derived metabolites by high-performance liquid chromatography coupled with tandem mass spectrometry revealed that serotonin and its hydroxycinnamic acid amides were accumulated in infected leaves. Serotonin accumulation was preceded by a transient increase in the tryptamine content and by marked activation of Trp decarboxylase, indicating that enhanced Trp production is linked to the formation of serotonin from Trp via tryptamine. Feeding of radiolabeled serotonin to inoculated leaves demonstrated that serotonin is incorporated into the cell walls of lesion tissue. The leaves of a propagating-type lesion mimic mutant (sl, Sekiguchi lesion) lacked both serotonin production and deposition of unextractable brown material at the infection sites, and showed increased susceptibility to B. oryzae infection. Treating the mutant with serotonin restored deposition of brown material at the lesion site. In addition, the serotonin treatment suppressed the growth of fungal hyphae in the leaf tissues of the sl mutant. These findings indicated that the activation of the Trp pathway is involved in the establishment of effective physical defenses by producing serotonin in rice leaves. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/18266919/The_tryptophan_pathway_is_involved_in_the_defense_responses_of_rice_against_pathogenic_infection_via_serotonin_production_ DB - PRIME DP - Unbound Medicine ER -