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Serum concentration of amino acids versus nutritional status in hemodialysis patients.
J Ren Nutr. 2008 Mar; 18(2):239-47.JR

Abstract

OBJECTIVE

The aim of this study was to evaluate the correlation between the serum concentration of amino acids (AAs) and nutritional status in hemodialysis (HD) patients.

METHODS

This study was performed in 22 HD patients dialyzed for 10 to 288 months, and in a control group of 20 healthy volunteers. Nutritional status was determined by the subjective global assessment method and by measuring albumin concentration. Body composition was determined using the parameters of body mass index, and the percentage of body fat and lean body mass (as measured by the near-infrared method). We measured C-reactive protein (CRP) as a marker of inflammatory status. Serum concentrations of 20 AAs were measured by precolumn orthophtalaldehyde derivatization, applying high-performance liquid chromatography (Hitachi-Merck HPLC, Tokyo, Japan) equipped with a C-18 reversed-phase column and a methanol/acetate buffer gradient.

RESULTS

Thirteen of 22 (59%) patients were of good nutritional status, and 9/22 (41%) were malnourished, including 1 person with severe malnutrition. In dialyzed patients compared with control subjects, a decreased concentration of essential and nonessential AAs was observed (P < .05). Concentrations of the majority of studied AAs (16 out of 20) were lower in patients dialyzed for a period >2 years, compared with patients dialyzed for a shorter time. The ratio of branched-chain amino acids (BCAAs) to aromatic AAs was lower in the dialyzed group compared with control subjects. This ratio was also lower in patients dialyzed longer compared with patients dialyzed for <2 years. No correlation between the concentration of some AAs and CRP level or dialysis adequacy was observed. In the malnourished group, an insignificantly lower concentration of some essential AAs (lysine, leucine, isoleucine, valine, and threonine), and a significantly higher (P = .04) concentration of CRP, were observed.

CONCLUSION

Despite quite good nutritional status, dialyzed patients present abnormalities in their AA profiles. Moreover, a significant decrease of BCAA concentration is related to calorie-protein malnutrition, inflammation, and a long period of hemodialysis.

Authors+Show Affiliations

Department of Clinical Nutrition and Diagnostic Laboratory, Medical University of Gdansk, Gdansk, Poland. sylwia@tetra.plNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18267217

Citation

Małgorzewicz, Sylwia, et al. "Serum Concentration of Amino Acids Versus Nutritional Status in Hemodialysis Patients." Journal of Renal Nutrition : the Official Journal of the Council On Renal Nutrition of the National Kidney Foundation, vol. 18, no. 2, 2008, pp. 239-47.
Małgorzewicz S, Debska-Slizień A, Rutkowski B, et al. Serum concentration of amino acids versus nutritional status in hemodialysis patients. J Ren Nutr. 2008;18(2):239-47.
Małgorzewicz, S., Debska-Slizień, A., Rutkowski, B., & Lysiak-Szydłowska, W. (2008). Serum concentration of amino acids versus nutritional status in hemodialysis patients. Journal of Renal Nutrition : the Official Journal of the Council On Renal Nutrition of the National Kidney Foundation, 18(2), 239-47. https://doi.org/10.1053/j.jrn.2007.11.011
Małgorzewicz S, et al. Serum Concentration of Amino Acids Versus Nutritional Status in Hemodialysis Patients. J Ren Nutr. 2008;18(2):239-47. PubMed PMID: 18267217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum concentration of amino acids versus nutritional status in hemodialysis patients. AU - Małgorzewicz,Sylwia, AU - Debska-Slizień,Alicja, AU - Rutkowski,Bolesław, AU - Lysiak-Szydłowska,Wiesława, PY - 2007/01/04/received PY - 2008/2/13/pubmed PY - 2008/3/18/medline PY - 2008/2/13/entrez SP - 239 EP - 47 JF - Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation JO - J Ren Nutr VL - 18 IS - 2 N2 - OBJECTIVE: The aim of this study was to evaluate the correlation between the serum concentration of amino acids (AAs) and nutritional status in hemodialysis (HD) patients. METHODS: This study was performed in 22 HD patients dialyzed for 10 to 288 months, and in a control group of 20 healthy volunteers. Nutritional status was determined by the subjective global assessment method and by measuring albumin concentration. Body composition was determined using the parameters of body mass index, and the percentage of body fat and lean body mass (as measured by the near-infrared method). We measured C-reactive protein (CRP) as a marker of inflammatory status. Serum concentrations of 20 AAs were measured by precolumn orthophtalaldehyde derivatization, applying high-performance liquid chromatography (Hitachi-Merck HPLC, Tokyo, Japan) equipped with a C-18 reversed-phase column and a methanol/acetate buffer gradient. RESULTS: Thirteen of 22 (59%) patients were of good nutritional status, and 9/22 (41%) were malnourished, including 1 person with severe malnutrition. In dialyzed patients compared with control subjects, a decreased concentration of essential and nonessential AAs was observed (P < .05). Concentrations of the majority of studied AAs (16 out of 20) were lower in patients dialyzed for a period >2 years, compared with patients dialyzed for a shorter time. The ratio of branched-chain amino acids (BCAAs) to aromatic AAs was lower in the dialyzed group compared with control subjects. This ratio was also lower in patients dialyzed longer compared with patients dialyzed for <2 years. No correlation between the concentration of some AAs and CRP level or dialysis adequacy was observed. In the malnourished group, an insignificantly lower concentration of some essential AAs (lysine, leucine, isoleucine, valine, and threonine), and a significantly higher (P = .04) concentration of CRP, were observed. CONCLUSION: Despite quite good nutritional status, dialyzed patients present abnormalities in their AA profiles. Moreover, a significant decrease of BCAA concentration is related to calorie-protein malnutrition, inflammation, and a long period of hemodialysis. SN - 1532-8503 UR - https://www.unboundmedicine.com/medline/citation/18267217/Serum_concentration_of_amino_acids_versus_nutritional_status_in_hemodialysis_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1051-2276(07)00269-5 DB - PRIME DP - Unbound Medicine ER -