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Overcoming H-NS-mediated transcriptional silencing of horizontally acquired genes by the PhoP and SlyA proteins in Salmonella enterica.
J Biol Chem. 2008 Apr 18; 283(16):10773-83.JB

Abstract

The acquisition of new traits through horizontal gene transfer depends on the ability of the recipient organism to express the incorporated genes. However, foreign DNA appears to be silenced by the histone-like nucleoid-structuring protein (H-NS) in several enteric pathogens, raising the question of how this silencing is overcome and the acquired genes are expressed at the right time and place. To address this question, we investigated transcription of the horizontally acquired ugtL and pagC genes from Salmonella enterica, which is dependent on the regulatory DNA-binding proteins PhoP and SlyA. We reconstituted transcription of the ugtL and pagC genes in vitro and determined occupancy of their respective promoters by PhoP, H-NS, and RNA polymerase in vivo. The SlyA protein counteracted H-NS-promoted repression in vitro but could not promote gene transcription by itself. PhoP-promoted transcription required SlyA when H-NS was present but not in its absence. In vivo, H-NS remained bound to the ugtL and pagC promoters under inducing conditions that promoted RNA polymerase recruitment and transcription of the ugtL and pagC genes. Our results indicate that relief of H-NS repression and recruitment of RNA polymerase are controlled by different regulatory proteins that act in concert to express horizontally acquired genes.

Authors+Show Affiliations

Program in Molecular Genetics and Genomics and Department of Molecular Microbiology, Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

18270203

Citation

Perez, J Christian, et al. "Overcoming H-NS-mediated Transcriptional Silencing of Horizontally Acquired Genes By the PhoP and SlyA Proteins in Salmonella Enterica." The Journal of Biological Chemistry, vol. 283, no. 16, 2008, pp. 10773-83.
Perez JC, Latifi T, Groisman EA. Overcoming H-NS-mediated transcriptional silencing of horizontally acquired genes by the PhoP and SlyA proteins in Salmonella enterica. J Biol Chem. 2008;283(16):10773-83.
Perez, J. C., Latifi, T., & Groisman, E. A. (2008). Overcoming H-NS-mediated transcriptional silencing of horizontally acquired genes by the PhoP and SlyA proteins in Salmonella enterica. The Journal of Biological Chemistry, 283(16), 10773-83. https://doi.org/10.1074/jbc.M709843200
Perez JC, Latifi T, Groisman EA. Overcoming H-NS-mediated Transcriptional Silencing of Horizontally Acquired Genes By the PhoP and SlyA Proteins in Salmonella Enterica. J Biol Chem. 2008 Apr 18;283(16):10773-83. PubMed PMID: 18270203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overcoming H-NS-mediated transcriptional silencing of horizontally acquired genes by the PhoP and SlyA proteins in Salmonella enterica. AU - Perez,J Christian, AU - Latifi,Tammy, AU - Groisman,Eduardo A, Y1 - 2008/02/11/ PY - 2008/2/14/pubmed PY - 2008/6/11/medline PY - 2008/2/14/entrez SP - 10773 EP - 83 JF - The Journal of biological chemistry JO - J Biol Chem VL - 283 IS - 16 N2 - The acquisition of new traits through horizontal gene transfer depends on the ability of the recipient organism to express the incorporated genes. However, foreign DNA appears to be silenced by the histone-like nucleoid-structuring protein (H-NS) in several enteric pathogens, raising the question of how this silencing is overcome and the acquired genes are expressed at the right time and place. To address this question, we investigated transcription of the horizontally acquired ugtL and pagC genes from Salmonella enterica, which is dependent on the regulatory DNA-binding proteins PhoP and SlyA. We reconstituted transcription of the ugtL and pagC genes in vitro and determined occupancy of their respective promoters by PhoP, H-NS, and RNA polymerase in vivo. The SlyA protein counteracted H-NS-promoted repression in vitro but could not promote gene transcription by itself. PhoP-promoted transcription required SlyA when H-NS was present but not in its absence. In vivo, H-NS remained bound to the ugtL and pagC promoters under inducing conditions that promoted RNA polymerase recruitment and transcription of the ugtL and pagC genes. Our results indicate that relief of H-NS repression and recruitment of RNA polymerase are controlled by different regulatory proteins that act in concert to express horizontally acquired genes. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/18270203/Overcoming_H_NS_mediated_transcriptional_silencing_of_horizontally_acquired_genes_by_the_PhoP_and_SlyA_proteins_in_Salmonella_enterica_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)62046-4 DB - PRIME DP - Unbound Medicine ER -