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Ceruloplasmin, an indicator of copper status.
Biol Trace Elem Res 2008; 123(1-3):261-9BT

Abstract

For clinical purposes, the non-ceruloplasmin copper fraction is routinely derived on the basis that ceruloplasmin binds six Cu atoms. However, this approach is limited because the actual ceruloplasmin copper binding is unclear. We performed direct measurement of the total serum copper and ceruloplasmin in 790 healthy individuals. We used an immunoprecipitation technique to separate ceruloplasmin and determined Cu content. With these values, we calculated the Cu/ceruloplasmin (Cp) ratio and thus generated data to support or discard the theoretical calculation of the non-ceruloplasmin fraction. Average of serum Cu and Cp levels were 18.4 +/- 4.4 micromol/l and 390 +/- 100 mg/l, respectively. The immunoprecipitation procedure allowed us to calculate a Cu/Cp ratio of 5.8, respectively, which supports the methodology of calculation that assigns a mean of six copper atoms to each ceruloplasmin molecule. With these values, we calculated that, in apparently normal adults, the non-ceruloplasmin copper (NCPC) fraction is lower than 1.3 micromol/l of Cu. In this report, we examine the Cp/Cu ratio by using Cp immunoprecipitation procedure. Our in vitro and in vivo studies indicate that, as a mean, there are 5.8 atoms of Cu per Cp molecule and that <1.3 micromol/l of Cu would correspond to the NCPC.

Authors+Show Affiliations

Micronutrient Laboratory, Nutrition and Food Technology Institute (INTA), University of Chile, El Líbano, 5524, Macul, Santiago, Chile. marredon@inta.clNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18270661

Citation

Arredondo, Miguel, et al. "Ceruloplasmin, an Indicator of Copper Status." Biological Trace Element Research, vol. 123, no. 1-3, 2008, pp. 261-9.
Arredondo M, González M, Olivares M, et al. Ceruloplasmin, an indicator of copper status. Biol Trace Elem Res. 2008;123(1-3):261-9.
Arredondo, M., González, M., Olivares, M., Pizarro, F., & Araya, M. (2008). Ceruloplasmin, an indicator of copper status. Biological Trace Element Research, 123(1-3), pp. 261-9. doi:10.1007/s12011-008-8110-2.
Arredondo M, et al. Ceruloplasmin, an Indicator of Copper Status. Biol Trace Elem Res. 2008;123(1-3):261-9. PubMed PMID: 18270661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ceruloplasmin, an indicator of copper status. AU - Arredondo,Miguel, AU - González,Mauricio, AU - Olivares,Manuel, AU - Pizarro,Fernando, AU - Araya,Magdalena, Y1 - 2008/02/13/ PY - 2007/12/13/received PY - 2008/01/24/accepted PY - 2008/2/14/pubmed PY - 2008/8/5/medline PY - 2008/2/14/entrez SP - 261 EP - 9 JF - Biological trace element research JO - Biol Trace Elem Res VL - 123 IS - 1-3 N2 - For clinical purposes, the non-ceruloplasmin copper fraction is routinely derived on the basis that ceruloplasmin binds six Cu atoms. However, this approach is limited because the actual ceruloplasmin copper binding is unclear. We performed direct measurement of the total serum copper and ceruloplasmin in 790 healthy individuals. We used an immunoprecipitation technique to separate ceruloplasmin and determined Cu content. With these values, we calculated the Cu/ceruloplasmin (Cp) ratio and thus generated data to support or discard the theoretical calculation of the non-ceruloplasmin fraction. Average of serum Cu and Cp levels were 18.4 +/- 4.4 micromol/l and 390 +/- 100 mg/l, respectively. The immunoprecipitation procedure allowed us to calculate a Cu/Cp ratio of 5.8, respectively, which supports the methodology of calculation that assigns a mean of six copper atoms to each ceruloplasmin molecule. With these values, we calculated that, in apparently normal adults, the non-ceruloplasmin copper (NCPC) fraction is lower than 1.3 micromol/l of Cu. In this report, we examine the Cp/Cu ratio by using Cp immunoprecipitation procedure. Our in vitro and in vivo studies indicate that, as a mean, there are 5.8 atoms of Cu per Cp molecule and that <1.3 micromol/l of Cu would correspond to the NCPC. SN - 0163-4984 UR - https://www.unboundmedicine.com/medline/citation/18270661/Ceruloplasmin_an_indicator_of_copper_status_ L2 - https://dx.doi.org/10.1007/s12011-008-8110-2 DB - PRIME DP - Unbound Medicine ER -