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Coupling of non-aqueous electrokinetic chromatography using cationic cyclodextrins with electrospray ionization mass spectrometry.
Rapid Commun Mass Spectrom. 2008; 22(6):790-6.RC

Abstract

Non-aqueous electrokinetic chromatography (NAEKC) using cationic cyclodextrins (CDs) was coupled to electrospray ionization mass spectrometry (ESI-MS). A methanolic background electrolyte (BGE) was used which contained the hydrochloride salts of the single-isomer derivative cyclodextrins 6-monodeoxy-6-mono(2-hydroxy)propylamino-beta-cyclodextrin (IPA-beta-CD) or 6-monodeoxy-6-mono(3-hydroxy)propylamino-beta-cyclodextrin (PA-beta-CD). Applying a reversed capillary electrophoresis (CE) polarity (-30 kV), efficient separation of negatively charged compounds was achieved with plate numbers of up to 190,000. PA-beta-CD appeared to be the most suitable for the separation of various acidic drugs while also providing a high chiral selectivity. Analyte detection was achieved by ESI-MS in the negative-ion mode using a sheath-liquid interface. In order to prevent current drops caused by the cathodic electroosmotic flow, a pressure of 15 mbar was applied on the inlet vial during NAEKC/MS analysis. The effect of the cationic CDs on the MS signal intensities of acidic test drugs was thoroughly studied. When a voltage is applied across the CE capillary, the overall mobility of the cationic CDs is towards the inlet vial so that no CD molecules enter the ion source. The chloride counter ions of the CDs, which migrated towards the capillary outlet, were found to cause ionization suppression, although significant analyte signals could still be detected. Depending on the CD concentration in the BGE, limits of detection for acidic drugs were in the 50-400 ng/mL range in full-scan mode.

Authors+Show Affiliations

Biomedical Analysis, Utrecht University, P.O. Box 80082, NL-3508 TB Utrecht, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18275093

Citation

Mol, Roelof, et al. "Coupling of Non-aqueous Electrokinetic Chromatography Using Cationic Cyclodextrins With Electrospray Ionization Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 6, 2008, pp. 790-6.
Mol R, de Jong GJ, Somsen GW. Coupling of non-aqueous electrokinetic chromatography using cationic cyclodextrins with electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom. 2008;22(6):790-6.
Mol, R., de Jong, G. J., & Somsen, G. W. (2008). Coupling of non-aqueous electrokinetic chromatography using cationic cyclodextrins with electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 22(6), 790-6. https://doi.org/10.1002/rcm.3431
Mol R, de Jong GJ, Somsen GW. Coupling of Non-aqueous Electrokinetic Chromatography Using Cationic Cyclodextrins With Electrospray Ionization Mass Spectrometry. Rapid Commun Mass Spectrom. 2008;22(6):790-6. PubMed PMID: 18275093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coupling of non-aqueous electrokinetic chromatography using cationic cyclodextrins with electrospray ionization mass spectrometry. AU - Mol,Roelof, AU - de Jong,Gerhardus J, AU - Somsen,Govert W, PY - 2008/2/16/pubmed PY - 2008/5/30/medline PY - 2008/2/16/entrez SP - 790 EP - 6 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 6 N2 - Non-aqueous electrokinetic chromatography (NAEKC) using cationic cyclodextrins (CDs) was coupled to electrospray ionization mass spectrometry (ESI-MS). A methanolic background electrolyte (BGE) was used which contained the hydrochloride salts of the single-isomer derivative cyclodextrins 6-monodeoxy-6-mono(2-hydroxy)propylamino-beta-cyclodextrin (IPA-beta-CD) or 6-monodeoxy-6-mono(3-hydroxy)propylamino-beta-cyclodextrin (PA-beta-CD). Applying a reversed capillary electrophoresis (CE) polarity (-30 kV), efficient separation of negatively charged compounds was achieved with plate numbers of up to 190,000. PA-beta-CD appeared to be the most suitable for the separation of various acidic drugs while also providing a high chiral selectivity. Analyte detection was achieved by ESI-MS in the negative-ion mode using a sheath-liquid interface. In order to prevent current drops caused by the cathodic electroosmotic flow, a pressure of 15 mbar was applied on the inlet vial during NAEKC/MS analysis. The effect of the cationic CDs on the MS signal intensities of acidic test drugs was thoroughly studied. When a voltage is applied across the CE capillary, the overall mobility of the cationic CDs is towards the inlet vial so that no CD molecules enter the ion source. The chloride counter ions of the CDs, which migrated towards the capillary outlet, were found to cause ionization suppression, although significant analyte signals could still be detected. Depending on the CD concentration in the BGE, limits of detection for acidic drugs were in the 50-400 ng/mL range in full-scan mode. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18275093/Coupling_of_non_aqueous_electrokinetic_chromatography_using_cationic_cyclodextrins_with_electrospray_ionization_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -