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The charge distribution in the cytoplasmic loop of subunit C of the F1F0 ATPase is a determinant for YidC targeting.
J Biol Chem 2008; 283(15):9871-7JB

Abstract

YidC is a member of the Oxa1 family of proteins that facilitates the membrane insertion of a subset of inner membrane proteins in Escherichia coli. YidC acts as an insertase for membrane insertion of subunit c of the F(1)F(0) ATP synthase (F(0)c), but the requirements for substrate recognition have remained unclear. Here, we have analyzed the role of the charged aminoacyl residues in F(0)c in YidC targeting and membrane insertion. Binding experiments demonstrate that F(0)c is targeted directly to YidC without the presence of a stable lipid surface-bound intermediate. Positive charges in the cytoplasmic loop of F(0)c are important determinants for YidC binding and subsequent membrane insertion. These data support a model in which F(0)c binds directly to YidC prior to its membrane insertion.

Authors+Show Affiliations

Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute of Advanced Materials, University of Groningen, Haren, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18276587

Citation

Kol, Stefan, et al. "The Charge Distribution in the Cytoplasmic Loop of Subunit C of the F1F0 ATPase Is a Determinant for YidC Targeting." The Journal of Biological Chemistry, vol. 283, no. 15, 2008, pp. 9871-7.
Kol S, Nouwen N, Driessen AJ. The charge distribution in the cytoplasmic loop of subunit C of the F1F0 ATPase is a determinant for YidC targeting. J Biol Chem. 2008;283(15):9871-7.
Kol, S., Nouwen, N., & Driessen, A. J. (2008). The charge distribution in the cytoplasmic loop of subunit C of the F1F0 ATPase is a determinant for YidC targeting. The Journal of Biological Chemistry, 283(15), pp. 9871-7. doi:10.1074/jbc.M709408200.
Kol S, Nouwen N, Driessen AJ. The Charge Distribution in the Cytoplasmic Loop of Subunit C of the F1F0 ATPase Is a Determinant for YidC Targeting. J Biol Chem. 2008 Apr 11;283(15):9871-7. PubMed PMID: 18276587.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The charge distribution in the cytoplasmic loop of subunit C of the F1F0 ATPase is a determinant for YidC targeting. AU - Kol,Stefan, AU - Nouwen,Nico, AU - Driessen,Arnold J M, Y1 - 2008/02/14/ PY - 2008/2/16/pubmed PY - 2008/5/29/medline PY - 2008/2/16/entrez SP - 9871 EP - 7 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 283 IS - 15 N2 - YidC is a member of the Oxa1 family of proteins that facilitates the membrane insertion of a subset of inner membrane proteins in Escherichia coli. YidC acts as an insertase for membrane insertion of subunit c of the F(1)F(0) ATP synthase (F(0)c), but the requirements for substrate recognition have remained unclear. Here, we have analyzed the role of the charged aminoacyl residues in F(0)c in YidC targeting and membrane insertion. Binding experiments demonstrate that F(0)c is targeted directly to YidC without the presence of a stable lipid surface-bound intermediate. Positive charges in the cytoplasmic loop of F(0)c are important determinants for YidC binding and subsequent membrane insertion. These data support a model in which F(0)c binds directly to YidC prior to its membrane insertion. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/18276587/The_charge_distribution_in_the_cytoplasmic_loop_of_subunit_C_of_the_F1F0_ATPase_is_a_determinant_for_YidC_targeting_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=18276587 DB - PRIME DP - Unbound Medicine ER -