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Type 1 fimbriae of insecticidal bacterium Xenorhabdus nematophila is necessary for growth and colonization of its symbiotic host nematode Steinernema carpocapsiae.
Environ Microbiol. 2008 May; 10(5):1285-95.EM

Abstract

Xenorhabdus nematophila produces type 1 fimbriae on the surface of Phase I cells. Fimbriae mediate recognition and adhesion of the bacteria to its target cell. To investigate the role of fimbriae in the biology of X. nematophila, we have produced a fimbrial mutant strain by insertional inactivation of the mrxA gene, encoding the structural subunit of type 1 fimbriae. Phenotypic characterization of the mutant revealed loss of fimbriae on the cell surface. Cell surface characteristics like dye absorption, biofilm formation, red blood cell agglutination remained unaltered. The mrxA mutant was defective in swarming on soft agar, although swimming motility was not affected. Flagellar expression was suppressed in the mrxA strain under swarming conditions, but not swimming conditions. Agglutination and cytotoxicity of the mutant to larval haemocytes was also reduced. When the mutant cells were injected in the haemocoel of the fourth instar larvae of Helicoverpa armigera, an increase in the LT(50) of 9-12 h was observed relative to the wild-type strain. The nematode growth was slow on the lawn of the fimbrial mutant. The mrxA negative strain was unable to colonize the nematode gut efficiently. This study demonstrates importance of type 1 fimbriae in establishment of bacteria-nematode symbiosis, a key to successful pest management program.

Authors+Show Affiliations

International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18279345

Citation

Chandra, Harish, et al. "Type 1 Fimbriae of Insecticidal Bacterium Xenorhabdus Nematophila Is Necessary for Growth and Colonization of Its Symbiotic Host Nematode Steinernema Carpocapsiae." Environmental Microbiology, vol. 10, no. 5, 2008, pp. 1285-95.
Chandra H, Khandelwal P, Khattri A, et al. Type 1 fimbriae of insecticidal bacterium Xenorhabdus nematophila is necessary for growth and colonization of its symbiotic host nematode Steinernema carpocapsiae. Environ Microbiol. 2008;10(5):1285-95.
Chandra, H., Khandelwal, P., Khattri, A., & Banerjee, N. (2008). Type 1 fimbriae of insecticidal bacterium Xenorhabdus nematophila is necessary for growth and colonization of its symbiotic host nematode Steinernema carpocapsiae. Environmental Microbiology, 10(5), 1285-95. https://doi.org/10.1111/j.1462-2920.2007.01542.x
Chandra H, et al. Type 1 Fimbriae of Insecticidal Bacterium Xenorhabdus Nematophila Is Necessary for Growth and Colonization of Its Symbiotic Host Nematode Steinernema Carpocapsiae. Environ Microbiol. 2008;10(5):1285-95. PubMed PMID: 18279345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Type 1 fimbriae of insecticidal bacterium Xenorhabdus nematophila is necessary for growth and colonization of its symbiotic host nematode Steinernema carpocapsiae. AU - Chandra,Harish, AU - Khandelwal,Puneet, AU - Khattri,Arun, AU - Banerjee,Nirupama, Y1 - 2008/02/14/ PY - 2008/2/19/pubmed PY - 2008/5/17/medline PY - 2008/2/19/entrez SP - 1285 EP - 95 JF - Environmental microbiology JO - Environ Microbiol VL - 10 IS - 5 N2 - Xenorhabdus nematophila produces type 1 fimbriae on the surface of Phase I cells. Fimbriae mediate recognition and adhesion of the bacteria to its target cell. To investigate the role of fimbriae in the biology of X. nematophila, we have produced a fimbrial mutant strain by insertional inactivation of the mrxA gene, encoding the structural subunit of type 1 fimbriae. Phenotypic characterization of the mutant revealed loss of fimbriae on the cell surface. Cell surface characteristics like dye absorption, biofilm formation, red blood cell agglutination remained unaltered. The mrxA mutant was defective in swarming on soft agar, although swimming motility was not affected. Flagellar expression was suppressed in the mrxA strain under swarming conditions, but not swimming conditions. Agglutination and cytotoxicity of the mutant to larval haemocytes was also reduced. When the mutant cells were injected in the haemocoel of the fourth instar larvae of Helicoverpa armigera, an increase in the LT(50) of 9-12 h was observed relative to the wild-type strain. The nematode growth was slow on the lawn of the fimbrial mutant. The mrxA negative strain was unable to colonize the nematode gut efficiently. This study demonstrates importance of type 1 fimbriae in establishment of bacteria-nematode symbiosis, a key to successful pest management program. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/18279345/Type_1_fimbriae_of_insecticidal_bacterium_Xenorhabdus_nematophila_is_necessary_for_growth_and_colonization_of_its_symbiotic_host_nematode_Steinernema_carpocapsiae_ L2 - https://doi.org/10.1111/j.1462-2920.2007.01542.x DB - PRIME DP - Unbound Medicine ER -