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Using oligonucleotide-functionalized Au nanoparticles to rapidly detect foodborne pathogens on a piezoelectric biosensor.
J Microbiol Methods. 2008 Apr; 73(1):7-17.JM

Abstract

A circulating-flow piezoelectric biosensor, based on an Au nanoparticle amplification and verification method, was used for real-time detection of a foodborne pathogen, Escherichia coli O157:H7. A synthesized thiolated probe (Probe 1; 30-mer) specific to E. coli O157:H7 eaeA gene was immobilized onto the piezoelectric biosensor surface. Hybridization was induced by exposing the immobilized probe to the E. coli O157:H7 eaeA gene fragment (104-bp) amplified by PCR, resulting in a mass change and a consequent frequency shift of the piezoelectric biosensor. A second thiolated probe (Probe 2), complementary to the target sequence, was conjugated to the Au nanoparticles and used as a "mass enhancer" and "sequence verifier" to amplify the frequency change of the piezoelectric biosensor. The PCR products amplified from concentrations of 1.2 x 10(2) CFU/ml of E. coli O157:H7 were detectable by the piezoelectric biosensor. A linear correlation was found when the E. coli O157:H7 detected from 10(2) to 10(6) CFU/ml. The piezoelectric biosensor was able to detect targets from real food samples.

Authors+Show Affiliations

Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30005, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18279983

Citation

Chen, Sz-Hau, et al. "Using Oligonucleotide-functionalized Au Nanoparticles to Rapidly Detect Foodborne Pathogens On a Piezoelectric Biosensor." Journal of Microbiological Methods, vol. 73, no. 1, 2008, pp. 7-17.
Chen SH, Wu VC, Chuang YC, et al. Using oligonucleotide-functionalized Au nanoparticles to rapidly detect foodborne pathogens on a piezoelectric biosensor. J Microbiol Methods. 2008;73(1):7-17.
Chen, S. H., Wu, V. C., Chuang, Y. C., & Lin, C. S. (2008). Using oligonucleotide-functionalized Au nanoparticles to rapidly detect foodborne pathogens on a piezoelectric biosensor. Journal of Microbiological Methods, 73(1), 7-17. https://doi.org/10.1016/j.mimet.2008.01.004
Chen SH, et al. Using Oligonucleotide-functionalized Au Nanoparticles to Rapidly Detect Foodborne Pathogens On a Piezoelectric Biosensor. J Microbiol Methods. 2008;73(1):7-17. PubMed PMID: 18279983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Using oligonucleotide-functionalized Au nanoparticles to rapidly detect foodborne pathogens on a piezoelectric biosensor. AU - Chen,Sz-Hau, AU - Wu,Vivian C H, AU - Chuang,Yao-Chen, AU - Lin,Chih-Sheng, Y1 - 2008/01/18/ PY - 2007/09/25/received PY - 2008/01/03/revised PY - 2008/01/09/accepted PY - 2008/2/19/pubmed PY - 2008/6/25/medline PY - 2008/2/19/entrez SP - 7 EP - 17 JF - Journal of microbiological methods JO - J Microbiol Methods VL - 73 IS - 1 N2 - A circulating-flow piezoelectric biosensor, based on an Au nanoparticle amplification and verification method, was used for real-time detection of a foodborne pathogen, Escherichia coli O157:H7. A synthesized thiolated probe (Probe 1; 30-mer) specific to E. coli O157:H7 eaeA gene was immobilized onto the piezoelectric biosensor surface. Hybridization was induced by exposing the immobilized probe to the E. coli O157:H7 eaeA gene fragment (104-bp) amplified by PCR, resulting in a mass change and a consequent frequency shift of the piezoelectric biosensor. A second thiolated probe (Probe 2), complementary to the target sequence, was conjugated to the Au nanoparticles and used as a "mass enhancer" and "sequence verifier" to amplify the frequency change of the piezoelectric biosensor. The PCR products amplified from concentrations of 1.2 x 10(2) CFU/ml of E. coli O157:H7 were detectable by the piezoelectric biosensor. A linear correlation was found when the E. coli O157:H7 detected from 10(2) to 10(6) CFU/ml. The piezoelectric biosensor was able to detect targets from real food samples. SN - 0167-7012 UR - https://www.unboundmedicine.com/medline/citation/18279983/Using_oligonucleotide_functionalized_Au_nanoparticles_to_rapidly_detect_foodborne_pathogens_on_a_piezoelectric_biosensor_ DB - PRIME DP - Unbound Medicine ER -