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Community-acquired methicillin-resistant Staphylococcus aureus in Madrid, Spain: transcontinental importation and polyclonal emergence of Panton-Valentine leukocidin-positive isolates.
Diagn Microbiol Infect Dis 2008; 61(2):143-9DM

Abstract

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates producing the Panton-Valentine leukocidin (PVL) have been reported worldwide. We describe the molecular characteristics of PVL-positive CA-MRSA strains isolated in Madrid, Spain, and analyze the clinical features of patients infected with these isolates. From 2004 to 2007, we collected 13 PVL-positive MRSA isolates from patients attending to the emergency department. The isolates were genotyped by pulsed-field gel electrophoresis, SCCmec typing, agr polymorphism, and multilocus sequence typing. Susceptibility to 29 antimicrobials was determined by the broth microdilution and by the E-test methods. The isolates belonged to 3 genotypes: ST8-SCCmec IVc (n = 11), ST5-SCCmec IVa (n = 1), and ST80-SCCmec IVc (n = 1). The corresponding agr types were I, II, and III, respectively. Five isolates were resistant to tetracycline and doxycycline, and 1 was resistant to fusidic acid (ST80). The isolates were from children (n = 9) and adults (n = 4), and were associated with skin and soft tissue infections (n = 9), otitis (n = 1), and bacteremia (n = 1). Nine patients were from South America. Our results indicate the transcontinental importation and recent emergence in Spain of PVL-positive CA-MRSA strains belonging to 3 distinct lineages, including 1 predominant (ST8-SCCmec IVc).

Authors+Show Affiliations

Servicio de Microbiología, Hospital General Universitario Gregorio Marañón, Madrid, Spain. ecercenado@terra.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18280081

Citation

Cercenado, Emilia, et al. "Community-acquired Methicillin-resistant Staphylococcus Aureus in Madrid, Spain: Transcontinental Importation and Polyclonal Emergence of Panton-Valentine Leukocidin-positive Isolates." Diagnostic Microbiology and Infectious Disease, vol. 61, no. 2, 2008, pp. 143-9.
Cercenado E, Cuevas O, Marín M, et al. Community-acquired methicillin-resistant Staphylococcus aureus in Madrid, Spain: transcontinental importation and polyclonal emergence of Panton-Valentine leukocidin-positive isolates. Diagn Microbiol Infect Dis. 2008;61(2):143-9.
Cercenado, E., Cuevas, O., Marín, M., Bouza, E., Trincado, P., Boquete, T., ... Vindel, A. (2008). Community-acquired methicillin-resistant Staphylococcus aureus in Madrid, Spain: transcontinental importation and polyclonal emergence of Panton-Valentine leukocidin-positive isolates. Diagnostic Microbiology and Infectious Disease, 61(2), pp. 143-9. doi:10.1016/j.diagmicrobio.2008.01.001.
Cercenado E, et al. Community-acquired Methicillin-resistant Staphylococcus Aureus in Madrid, Spain: Transcontinental Importation and Polyclonal Emergence of Panton-Valentine Leukocidin-positive Isolates. Diagn Microbiol Infect Dis. 2008;61(2):143-9. PubMed PMID: 18280081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Community-acquired methicillin-resistant Staphylococcus aureus in Madrid, Spain: transcontinental importation and polyclonal emergence of Panton-Valentine leukocidin-positive isolates. AU - Cercenado,Emilia, AU - Cuevas,Oscar, AU - Marín,Mercedes, AU - Bouza,Emilio, AU - Trincado,Pilar, AU - Boquete,Teresa, AU - Padilla,Belén, AU - Vindel,Ana, Y1 - 2008/02/15/ PY - 2007/10/24/received PY - 2007/12/19/revised PY - 2008/01/07/accepted PY - 2008/2/19/pubmed PY - 2008/7/25/medline PY - 2008/2/19/entrez SP - 143 EP - 9 JF - Diagnostic microbiology and infectious disease JO - Diagn. Microbiol. Infect. Dis. VL - 61 IS - 2 N2 - Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates producing the Panton-Valentine leukocidin (PVL) have been reported worldwide. We describe the molecular characteristics of PVL-positive CA-MRSA strains isolated in Madrid, Spain, and analyze the clinical features of patients infected with these isolates. From 2004 to 2007, we collected 13 PVL-positive MRSA isolates from patients attending to the emergency department. The isolates were genotyped by pulsed-field gel electrophoresis, SCCmec typing, agr polymorphism, and multilocus sequence typing. Susceptibility to 29 antimicrobials was determined by the broth microdilution and by the E-test methods. The isolates belonged to 3 genotypes: ST8-SCCmec IVc (n = 11), ST5-SCCmec IVa (n = 1), and ST80-SCCmec IVc (n = 1). The corresponding agr types were I, II, and III, respectively. Five isolates were resistant to tetracycline and doxycycline, and 1 was resistant to fusidic acid (ST80). The isolates were from children (n = 9) and adults (n = 4), and were associated with skin and soft tissue infections (n = 9), otitis (n = 1), and bacteremia (n = 1). Nine patients were from South America. Our results indicate the transcontinental importation and recent emergence in Spain of PVL-positive CA-MRSA strains belonging to 3 distinct lineages, including 1 predominant (ST8-SCCmec IVc). SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/18280081/Community_acquired_methicillin_resistant_Staphylococcus_aureus_in_Madrid_Spain:_transcontinental_importation_and_polyclonal_emergence_of_Panton_Valentine_leukocidin_positive_isolates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(08)00029-1 DB - PRIME DP - Unbound Medicine ER -