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In vitro activity of antibiotic combinations against Pseudomonas aeruginosa biofilm and planktonic cultures.
Int J Antimicrob Agents. 2008 Apr; 31(4):329-36.IJ

Abstract

In order to investigate the hypothesis that the combination of clarithromycin with other antibacterial agents offers a successful treatment in the eradication of Pseudomonas aeruginosa biofilm, we first determined the activity of eight antimicrobial agents against planktonic cultures of P. aeruginosa isolates by the microdilution technique. Second, we determined the in vitro effects of these antimicrobial agents individually and in combination against planktonic cultures and pre-formed biofilms of P. aeruginosa PA01. Drug combinations with marked activity were tested on biofilms of clinical isolates. The percentages of planktonic culture survival and biofilm persistence were determined using spectrophotometry and scanning electron microscopy, respectively. Bacterial enumeration was used as a more quantitative method to assess the viability of bacteria in the biofilm. Among the antibacterial agents tested, tobramycin and polymyxin B had the strongest activity against planktonic cultures when tested alone. Synergistic activity was observed for the combination chitosan/tobramycin against planktonic cultures but not biofilms, and for the combination tobramycin/clarithromycin against biofilms but not planktonic cultures. The results suggest that the combination clarithromycin/tobramycin may be successful for eradicating infections involving bacterial biofilms such as in cystic fibrosis patients chronically infected by P. aeruginosa. Further studies on representative isolates in vivo are warranted to support these results.

Authors+Show Affiliations

Pharmaceutical Microbiology Laboratory, Institute of Pharmacy, Université Libre de Bruxelles, CP 205/02, Boulevard du Triomphe, 1050 Brussels, Belgium. marie.tre-hardy@ulb.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18280117

Citation

Tré-Hardy, Marie, et al. "In Vitro Activity of Antibiotic Combinations Against Pseudomonas Aeruginosa Biofilm and Planktonic Cultures." International Journal of Antimicrobial Agents, vol. 31, no. 4, 2008, pp. 329-36.
Tré-Hardy M, Vanderbist F, Traore H, et al. In vitro activity of antibiotic combinations against Pseudomonas aeruginosa biofilm and planktonic cultures. Int J Antimicrob Agents. 2008;31(4):329-36.
Tré-Hardy, M., Vanderbist, F., Traore, H., & Devleeschouwer, M. J. (2008). In vitro activity of antibiotic combinations against Pseudomonas aeruginosa biofilm and planktonic cultures. International Journal of Antimicrobial Agents, 31(4), 329-36. https://doi.org/10.1016/j.ijantimicag.2007.12.005
Tré-Hardy M, et al. In Vitro Activity of Antibiotic Combinations Against Pseudomonas Aeruginosa Biofilm and Planktonic Cultures. Int J Antimicrob Agents. 2008;31(4):329-36. PubMed PMID: 18280117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro activity of antibiotic combinations against Pseudomonas aeruginosa biofilm and planktonic cultures. AU - Tré-Hardy,Marie, AU - Vanderbist,Francis, AU - Traore,Hamidou, AU - Devleeschouwer,Michel Jean, Y1 - 2008/02/14/ PY - 2007/11/08/received PY - 2007/12/05/revised PY - 2007/12/06/accepted PY - 2008/2/19/pubmed PY - 2008/7/17/medline PY - 2008/2/19/entrez SP - 329 EP - 36 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 31 IS - 4 N2 - In order to investigate the hypothesis that the combination of clarithromycin with other antibacterial agents offers a successful treatment in the eradication of Pseudomonas aeruginosa biofilm, we first determined the activity of eight antimicrobial agents against planktonic cultures of P. aeruginosa isolates by the microdilution technique. Second, we determined the in vitro effects of these antimicrobial agents individually and in combination against planktonic cultures and pre-formed biofilms of P. aeruginosa PA01. Drug combinations with marked activity were tested on biofilms of clinical isolates. The percentages of planktonic culture survival and biofilm persistence were determined using spectrophotometry and scanning electron microscopy, respectively. Bacterial enumeration was used as a more quantitative method to assess the viability of bacteria in the biofilm. Among the antibacterial agents tested, tobramycin and polymyxin B had the strongest activity against planktonic cultures when tested alone. Synergistic activity was observed for the combination chitosan/tobramycin against planktonic cultures but not biofilms, and for the combination tobramycin/clarithromycin against biofilms but not planktonic cultures. The results suggest that the combination clarithromycin/tobramycin may be successful for eradicating infections involving bacterial biofilms such as in cystic fibrosis patients chronically infected by P. aeruginosa. Further studies on representative isolates in vivo are warranted to support these results. SN - 0924-8579 UR - https://www.unboundmedicine.com/medline/citation/18280117/In_vitro_activity_of_antibiotic_combinations_against_Pseudomonas_aeruginosa_biofilm_and_planktonic_cultures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(08)00013-7 DB - PRIME DP - Unbound Medicine ER -