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Solid-phase extraction procedure for determination of phenolic acids and some flavonols in honey.
J Chromatogr A. 2008 Apr 11; 1187(1-2):18-24.JC

Abstract

The solid-phase extraction procedure (SPE) for isolation and preconcentration of phenolic acids (gallic, p-HBA, p-coumaric, vanillic, caffeic and syringic acid) and some flavonols (rutin, quercetin and kaempferol) from honey samples prior to their determination by HPLC is reported. Different solid sorbents such as Bond Elut octadecyl C(18), Oasis HLB, Strata-X and Amberlite XAD-2 were tested for this purpose. The best results were obtain when aqueous solution of honey (100 mL) was adjusted to pH 2 and passed through the microcolumn containing 2.5 g of Oasis HB followed by washing the sorbent with 50 mL of acidified water (pH 2). The analytes were then eluted with methanol. The proposed method permits the quantification of the studied compounds with the limit of detections ranged from 25 ng kg(-1) to 0.75 microg kg(-1) for p-HBA and quercetin, respectively. The precision of the overall analytical procedure was estimated by measuring the within-day repeatability and the relative standard deviations of the parallel (n=3) results were in the range of 1.9-10.1%. The method was tested for real honey samples from different botanical origins.

Authors+Show Affiliations

Department of Chemistry, Warsaw University, Pasteura 1, 02-093 Warsaw, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18282581

Citation

Michalkiewicz, Anna, et al. "Solid-phase Extraction Procedure for Determination of Phenolic Acids and some Flavonols in Honey." Journal of Chromatography. A, vol. 1187, no. 1-2, 2008, pp. 18-24.
Michalkiewicz A, Biesaga M, Pyrzynska K. Solid-phase extraction procedure for determination of phenolic acids and some flavonols in honey. J Chromatogr A. 2008;1187(1-2):18-24.
Michalkiewicz, A., Biesaga, M., & Pyrzynska, K. (2008). Solid-phase extraction procedure for determination of phenolic acids and some flavonols in honey. Journal of Chromatography. A, 1187(1-2), 18-24. https://doi.org/10.1016/j.chroma.2008.02.001
Michalkiewicz A, Biesaga M, Pyrzynska K. Solid-phase Extraction Procedure for Determination of Phenolic Acids and some Flavonols in Honey. J Chromatogr A. 2008 Apr 11;1187(1-2):18-24. PubMed PMID: 18282581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solid-phase extraction procedure for determination of phenolic acids and some flavonols in honey. AU - Michalkiewicz,Anna, AU - Biesaga,Magdalena, AU - Pyrzynska,Krystyna, Y1 - 2008/02/07/ PY - 2007/11/12/received PY - 2008/01/30/revised PY - 2008/02/04/accepted PY - 2008/2/20/pubmed PY - 2008/6/27/medline PY - 2008/2/20/entrez SP - 18 EP - 24 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1187 IS - 1-2 N2 - The solid-phase extraction procedure (SPE) for isolation and preconcentration of phenolic acids (gallic, p-HBA, p-coumaric, vanillic, caffeic and syringic acid) and some flavonols (rutin, quercetin and kaempferol) from honey samples prior to their determination by HPLC is reported. Different solid sorbents such as Bond Elut octadecyl C(18), Oasis HLB, Strata-X and Amberlite XAD-2 were tested for this purpose. The best results were obtain when aqueous solution of honey (100 mL) was adjusted to pH 2 and passed through the microcolumn containing 2.5 g of Oasis HB followed by washing the sorbent with 50 mL of acidified water (pH 2). The analytes were then eluted with methanol. The proposed method permits the quantification of the studied compounds with the limit of detections ranged from 25 ng kg(-1) to 0.75 microg kg(-1) for p-HBA and quercetin, respectively. The precision of the overall analytical procedure was estimated by measuring the within-day repeatability and the relative standard deviations of the parallel (n=3) results were in the range of 1.9-10.1%. The method was tested for real honey samples from different botanical origins. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18282581/Solid_phase_extraction_procedure_for_determination_of_phenolic_acids_and_some_flavonols_in_honey_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00217-3 DB - PRIME DP - Unbound Medicine ER -