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Development of a reference material using methamphetamine abusers' hair samples for the determination of methamphetamine and amphetamine in hair.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 01; 865(1-2):33-9.JC

Abstract

In the present study, we developed a reference material (RM) using authentic hair samples for the determination of methamphetamine (MA) and its main metabolite, amphetamine (AP) in human hair. MA abusers' hair samples were collected, homogenized and finally bottled. The concentration of each bottle was determined using two extraction methods, agitation with 1% HCl in methanol at 38 degrees C and ultrasonication with methanol/5M HCl (20:1), followed by gas chromatography/mass spectrometry (GC-MS) after derivatization with trifluoroacetic anhydride (TFAA). Both analytical procedures were fully validated and their extraction efficiency was compared. The homogeneity of analytes was evaluated and their property values were determined with their uncertainties. The two methods were acceptable to analyze MA and AP in human hair through the validation and comparative studies using spiked and authentic hair samples as well as NIST SRM 2379 certified reference material. Satisfying homogeneity was reached for MA and AP in the prepared RM. Finally, a human hair RM containing MA and AP is prepared at the level of 7.64+/-1.24 and 0.54+/-0.07 ng/mg, respectively. This material can be useful in forensic laboratories for internal quality control and external quality assurance.

Authors+Show Affiliations

National Institute of Scientific Investigation, 331-1 Sinwol-7-dong, Yangcheon-gu, Seoul 158-707, Republic of Korea. spp26625@yahoo.co.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

18282747

Citation

Lee, Sooyeun, et al. "Development of a Reference Material Using Methamphetamine Abusers' Hair Samples for the Determination of Methamphetamine and Amphetamine in Hair." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 865, no. 1-2, 2008, pp. 33-9.
Lee S, Park Y, Yang W, et al. Development of a reference material using methamphetamine abusers' hair samples for the determination of methamphetamine and amphetamine in hair. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;865(1-2):33-9.
Lee, S., Park, Y., Yang, W., Han, E., Choe, S., In, S., Lim, M., & Chung, H. (2008). Development of a reference material using methamphetamine abusers' hair samples for the determination of methamphetamine and amphetamine in hair. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 865(1-2), 33-9. https://doi.org/10.1016/j.jchromb.2008.01.039
Lee S, et al. Development of a Reference Material Using Methamphetamine Abusers' Hair Samples for the Determination of Methamphetamine and Amphetamine in Hair. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):33-9. PubMed PMID: 18282747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a reference material using methamphetamine abusers' hair samples for the determination of methamphetamine and amphetamine in hair. AU - Lee,Sooyeun, AU - Park,Yonghoon, AU - Yang,Wonkyung, AU - Han,Eunyoung, AU - Choe,Sanggil, AU - In,Sangwhan, AU - Lim,Miae, AU - Chung,Heesun, Y1 - 2008/02/03/ PY - 2007/06/26/received PY - 2008/01/24/revised PY - 2008/01/25/accepted PY - 2008/2/20/pubmed PY - 2008/8/9/medline PY - 2008/2/20/entrez SP - 33 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 865 IS - 1-2 N2 - In the present study, we developed a reference material (RM) using authentic hair samples for the determination of methamphetamine (MA) and its main metabolite, amphetamine (AP) in human hair. MA abusers' hair samples were collected, homogenized and finally bottled. The concentration of each bottle was determined using two extraction methods, agitation with 1% HCl in methanol at 38 degrees C and ultrasonication with methanol/5M HCl (20:1), followed by gas chromatography/mass spectrometry (GC-MS) after derivatization with trifluoroacetic anhydride (TFAA). Both analytical procedures were fully validated and their extraction efficiency was compared. The homogeneity of analytes was evaluated and their property values were determined with their uncertainties. The two methods were acceptable to analyze MA and AP in human hair through the validation and comparative studies using spiked and authentic hair samples as well as NIST SRM 2379 certified reference material. Satisfying homogeneity was reached for MA and AP in the prepared RM. Finally, a human hair RM containing MA and AP is prepared at the level of 7.64+/-1.24 and 0.54+/-0.07 ng/mg, respectively. This material can be useful in forensic laboratories for internal quality control and external quality assurance. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18282747/Development_of_a_reference_material_using_methamphetamine_abusers'_hair_samples_for_the_determination_of_methamphetamine_and_amphetamine_in_hair_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00079-2 DB - PRIME DP - Unbound Medicine ER -