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In vivo DNA damage induced by the cyanotoxin microcystin-LR: comparison of intra-peritoneal and oral administrations by use of the comet assay.
Mutat Res. 2008 Mar 29; 652(1):65-71.MR

Abstract

Microcystin-LR (MC-LR), involved in human and animal poisonings by cyanobacteria, has been shown to be both a potent tumour promoter in rat liver and an inhibitor of serine/threonine protein phosphatases, specifically PP1 and PP2A. The research on the genotoxic potential of MC-LR counts only few in vivo studies. In order to determine the target organs for DNA-damage induction by MC-LR, the single-cell gel electrophoresis (SCGE) or comet assay was performed in mice. Following a single oral administration of 2 and 4mg/kg bw of MC-LR, a statistically significant induction of DNA damage in blood cells was obtained after 3h. However, after an intra-peritoneal injection (ip), DNA lesions were mainly induced in the liver, but were also reported in the kidney, the intestine and the colon. The sensitivity of the ip route compared to the oral route suggested a difference in the bio-disponibility of the toxin. In any case, DNA damage was induced by MC-LR irrespective of the administration route. Among the target organs, the DNA damage induced in the intestinal tissues (ileum and colon) may contribute to an increased cancer risk.

Authors+Show Affiliations

Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches sur les Médicaments Vétérinaires et les Désinfectants, Unité de Toxicologie Génétique des Contaminants Alimentaires, Fougères, France. julien.gaudin0064@orange.frNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18282792

Citation

Gaudin, Julien, et al. "In Vivo DNA Damage Induced By the Cyanotoxin microcystin-LR: Comparison of Intra-peritoneal and Oral Administrations By Use of the Comet Assay." Mutation Research, vol. 652, no. 1, 2008, pp. 65-71.
Gaudin J, Huet S, Jarry G, et al. In vivo DNA damage induced by the cyanotoxin microcystin-LR: comparison of intra-peritoneal and oral administrations by use of the comet assay. Mutat Res. 2008;652(1):65-71.
Gaudin, J., Huet, S., Jarry, G., & Fessard, V. (2008). In vivo DNA damage induced by the cyanotoxin microcystin-LR: comparison of intra-peritoneal and oral administrations by use of the comet assay. Mutation Research, 652(1), 65-71. https://doi.org/10.1016/j.mrgentox.2007.10.024
Gaudin J, et al. In Vivo DNA Damage Induced By the Cyanotoxin microcystin-LR: Comparison of Intra-peritoneal and Oral Administrations By Use of the Comet Assay. Mutat Res. 2008 Mar 29;652(1):65-71. PubMed PMID: 18282792.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo DNA damage induced by the cyanotoxin microcystin-LR: comparison of intra-peritoneal and oral administrations by use of the comet assay. AU - Gaudin,Julien, AU - Huet,Sylvie, AU - Jarry,Gérard, AU - Fessard,Valérie, Y1 - 2007/12/03/ PY - 2006/10/04/received PY - 2007/05/10/revised PY - 2007/10/15/accepted PY - 2008/2/20/pubmed PY - 2008/5/29/medline PY - 2008/2/20/entrez SP - 65 EP - 71 JF - Mutation research JO - Mutat Res VL - 652 IS - 1 N2 - Microcystin-LR (MC-LR), involved in human and animal poisonings by cyanobacteria, has been shown to be both a potent tumour promoter in rat liver and an inhibitor of serine/threonine protein phosphatases, specifically PP1 and PP2A. The research on the genotoxic potential of MC-LR counts only few in vivo studies. In order to determine the target organs for DNA-damage induction by MC-LR, the single-cell gel electrophoresis (SCGE) or comet assay was performed in mice. Following a single oral administration of 2 and 4mg/kg bw of MC-LR, a statistically significant induction of DNA damage in blood cells was obtained after 3h. However, after an intra-peritoneal injection (ip), DNA lesions were mainly induced in the liver, but were also reported in the kidney, the intestine and the colon. The sensitivity of the ip route compared to the oral route suggested a difference in the bio-disponibility of the toxin. In any case, DNA damage was induced by MC-LR irrespective of the administration route. Among the target organs, the DNA damage induced in the intestinal tissues (ileum and colon) may contribute to an increased cancer risk. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/18282792/In_vivo_DNA_damage_induced_by_the_cyanotoxin_microcystin_LR:_comparison_of_intra_peritoneal_and_oral_administrations_by_use_of_the_comet_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(07)00339-7 DB - PRIME DP - Unbound Medicine ER -