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A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women.
J Med Microbiol 2008; 57(Pt 3):304-9JM

Abstract

The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time Mycoplasma genitalium adhesin protein (MgPa) gene PCR as detection methods for M. genitalium infection. The study also determined the prevalence of M. genitalium in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First void urine (FVU) and/or urethral swabs were collected from 381 men, and FVU and/or cervical swabs and/or urethral swabs were collected from 298 women. A total of 213 specimens were used in the PCR comparative study: 98 consecutively sampled specimens from patients enrolled in the prevalence study, 36 consecutively sampled specimens from patients with symptoms of urethritis and 79 specimens from patients positive for M. genitalium by real-time MgPa gene PCR in the prevalence study. A true-positive M. genitalium DNA specimen was defined as either a specimen positive in any two PCR assays or a specimen whose PCR product was verified by DNA sequencing. The prevalence of M. genitalium infection in men and women was 27/381 (7.1 %) and 23/298 (7.7 %), respectively. In the PCR comparative study, M. genitalium DNA was detected in 61/76 (80.3 %) of true-positive specimens by conventional 16S rRNA gene PCR, in 52/76 (68.4 %) by real-time 16S rRNA gene PCR and in 74/76 (97.4 %) by real-time MgPa gene PCR. Real-time MgPa gene PCR thus had higher sensitivity compared with conventional 16S rRNA gene PCR and had considerably increased sensitivity compared with real-time 16S rRNA gene PCR for detection of M. genitalium DNA. Real-time MgPa gene PCR is well suited for the clinical diagnosis of M. genitalium.

Authors+Show Affiliations

Department of Clinical Microbiology, Central Hospital, Karlstad, Sweden. andreas.edberg@liv.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18287292

Citation

Edberg, Andreas, et al. "A Comparative Study of Three Different PCR Assays for Detection of Mycoplasma Genitalium in Urogenital Specimens From Men and Women." Journal of Medical Microbiology, vol. 57, no. Pt 3, 2008, pp. 304-9.
Edberg A, Jurstrand M, Johansson E, et al. A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women. J Med Microbiol. 2008;57(Pt 3):304-9.
Edberg, A., Jurstrand, M., Johansson, E., Wikander, E., Höög, A., Ahlqvist, T., ... Fredlund, H. (2008). A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women. Journal of Medical Microbiology, 57(Pt 3), pp. 304-9. doi:10.1099/jmm.0.47498-0.
Edberg A, et al. A Comparative Study of Three Different PCR Assays for Detection of Mycoplasma Genitalium in Urogenital Specimens From Men and Women. J Med Microbiol. 2008;57(Pt 3):304-9. PubMed PMID: 18287292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women. AU - Edberg,Andreas, AU - Jurstrand,Margaretha, AU - Johansson,Eva, AU - Wikander,Elisabeth, AU - Höög,Anna, AU - Ahlqvist,Thomas, AU - Falk,Lars, AU - Jensen,Jørgen Skov, AU - Fredlund,Hans, PY - 2008/2/22/pubmed PY - 2008/4/1/medline PY - 2008/2/22/entrez SP - 304 EP - 9 JF - Journal of medical microbiology JO - J. Med. Microbiol. VL - 57 IS - Pt 3 N2 - The aim of this study was to compare conventional 16S rRNA gene PCR, real-time 16S rRNA gene PCR and real-time Mycoplasma genitalium adhesin protein (MgPa) gene PCR as detection methods for M. genitalium infection. The study also determined the prevalence of M. genitalium in male and female patients attending a sexually transmitted infections clinic in a rural area in the west of Sweden. First void urine (FVU) and/or urethral swabs were collected from 381 men, and FVU and/or cervical swabs and/or urethral swabs were collected from 298 women. A total of 213 specimens were used in the PCR comparative study: 98 consecutively sampled specimens from patients enrolled in the prevalence study, 36 consecutively sampled specimens from patients with symptoms of urethritis and 79 specimens from patients positive for M. genitalium by real-time MgPa gene PCR in the prevalence study. A true-positive M. genitalium DNA specimen was defined as either a specimen positive in any two PCR assays or a specimen whose PCR product was verified by DNA sequencing. The prevalence of M. genitalium infection in men and women was 27/381 (7.1 %) and 23/298 (7.7 %), respectively. In the PCR comparative study, M. genitalium DNA was detected in 61/76 (80.3 %) of true-positive specimens by conventional 16S rRNA gene PCR, in 52/76 (68.4 %) by real-time 16S rRNA gene PCR and in 74/76 (97.4 %) by real-time MgPa gene PCR. Real-time MgPa gene PCR thus had higher sensitivity compared with conventional 16S rRNA gene PCR and had considerably increased sensitivity compared with real-time 16S rRNA gene PCR for detection of M. genitalium DNA. Real-time MgPa gene PCR is well suited for the clinical diagnosis of M. genitalium. SN - 0022-2615 UR - https://www.unboundmedicine.com/medline/citation/18287292/A_comparative_study_of_three_different_PCR_assays_for_detection_of_Mycoplasma_genitalium_in_urogenital_specimens_from_men_and_women_ L2 - http://jmm.microbiologyresearch.org/pubmed/content/journal/jmm/10.1099/jmm.0.47498-0 DB - PRIME DP - Unbound Medicine ER -