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Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide.
Environ Mol Mutagen. 2008 Apr; 49(3):217-25.EM

Abstract

An important endpoint in assessing pollution-related toxicity is genotoxicity. To obtain insight into the time-course of oxidative- and alkylation-induced DNA damage in the freshwater mussel, Unio pictorum, mussels were exposed for 24 hr to concentration gradients of pro-oxidant hydrogen peroxide (H(2)O(2)) and a mono-functional alkylating agent, ethyl methanesulfonate (EMS). DNA damage was assessed in haemocytes immediately upon exposure and over the recovery period of up to 72 days by means of comet and micronucleus assays. Following exposure to H(2)O(2), DNA damage as detected by the comet assay returned to control values after one day, except for the mussels exposed to the highest dose when damage was detectable for the next 3 days. In contrast, alkylation-induced DNA damage was detectable even after 72 days of recovery in de-chlorinated water, with a dose-response relationship observable throughout the whole recovery period. Micronucleus frequency was the highest on Day 3 after exposure to EMS; it decreased considerably by Day 7 and returned almost to the control levels 19 days after exposure, while no significant induction of micronuclei was observed in mussels exposed to H(2)O(2). Although the comet assay is considered a biomarker of recent genotoxic exposure, detecting DNA damage of shorter longevity than with the micronucleus assay, results presented here show that in the case of alkylation damage the comet assay reveals genotoxic exposure of U. pictorum in a dose-dependent manner even after 2 months.

Authors+Show Affiliations

Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18288719

Citation

Stambuk, Anamaria, et al. "Persistence of DNA Damage in the Freshwater Mussel Unio Pictorum Upon Exposure to Ethyl Methanesulphonate and Hydrogen Peroxide." Environmental and Molecular Mutagenesis, vol. 49, no. 3, 2008, pp. 217-25.
Stambuk A, Pavlica M, Malović L, et al. Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide. Environ Mol Mutagen. 2008;49(3):217-25.
Stambuk, A., Pavlica, M., Malović, L., & Klobucsar, G. I. (2008). Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide. Environmental and Molecular Mutagenesis, 49(3), 217-25. https://doi.org/10.1002/em.20376
Stambuk A, et al. Persistence of DNA Damage in the Freshwater Mussel Unio Pictorum Upon Exposure to Ethyl Methanesulphonate and Hydrogen Peroxide. Environ Mol Mutagen. 2008;49(3):217-25. PubMed PMID: 18288719.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Persistence of DNA damage in the freshwater mussel Unio pictorum upon exposure to ethyl methanesulphonate and hydrogen peroxide. AU - Stambuk,Anamaria, AU - Pavlica,Mirjana, AU - Malović,Lana, AU - Klobucsar,Göran I V, PY - 2008/2/22/pubmed PY - 2008/6/11/medline PY - 2008/2/22/entrez SP - 217 EP - 25 JF - Environmental and molecular mutagenesis JO - Environ Mol Mutagen VL - 49 IS - 3 N2 - An important endpoint in assessing pollution-related toxicity is genotoxicity. To obtain insight into the time-course of oxidative- and alkylation-induced DNA damage in the freshwater mussel, Unio pictorum, mussels were exposed for 24 hr to concentration gradients of pro-oxidant hydrogen peroxide (H(2)O(2)) and a mono-functional alkylating agent, ethyl methanesulfonate (EMS). DNA damage was assessed in haemocytes immediately upon exposure and over the recovery period of up to 72 days by means of comet and micronucleus assays. Following exposure to H(2)O(2), DNA damage as detected by the comet assay returned to control values after one day, except for the mussels exposed to the highest dose when damage was detectable for the next 3 days. In contrast, alkylation-induced DNA damage was detectable even after 72 days of recovery in de-chlorinated water, with a dose-response relationship observable throughout the whole recovery period. Micronucleus frequency was the highest on Day 3 after exposure to EMS; it decreased considerably by Day 7 and returned almost to the control levels 19 days after exposure, while no significant induction of micronuclei was observed in mussels exposed to H(2)O(2). Although the comet assay is considered a biomarker of recent genotoxic exposure, detecting DNA damage of shorter longevity than with the micronucleus assay, results presented here show that in the case of alkylation damage the comet assay reveals genotoxic exposure of U. pictorum in a dose-dependent manner even after 2 months. SN - 0893-6692 UR - https://www.unboundmedicine.com/medline/citation/18288719/Persistence_of_DNA_damage_in_the_freshwater_mussel_Unio_pictorum_upon_exposure_to_ethyl_methanesulphonate_and_hydrogen_peroxide_ L2 - https://doi.org/10.1002/em.20376 DB - PRIME DP - Unbound Medicine ER -