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Role of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit GluR1 in spinal dorsal horn in inflammatory nociception and neuropathic nociception in rat.
Brain Res. 2008 Mar 20; 1200:19-26.BR

Abstract

The present study aims to investigate changes of spinal cord AMPA receptor GluR1 and its phosphorylation in inflammatory and neuropathic pain. Complete Freund's adjuvant (CFA) injection into the hind paw produced inflammatory thermal hyperalgesia that was assessed by decreased response latency to radiant heat; spinal nerve ligation (SNL) was used to induce mechanical allodynia that was evaluated with von Frey hairs. By method of Western blot, expression of GluR1 (the main subunit of the AMPA receptor) and its phosphorylated forms at serine 845 (pGluR1-Ser845) and at serine 831 (pGluR1-Ser831) in the spinal dorsal horn was observed. It was found that the expression of pGluR1-Ser845 and pGluR1-Ser831 increased significantly at 1 h after CFA injection, reached peak at 4 h and returned to the normal control level at 24 h, while no significant change was detected in GluR1 itself. In contrast, neither GluR1 nor pGluR1 showed any significant change in rats following SNL. These results suggest that phosphorylated GluR1 (pGluR1-Ser845 and pGluR1-Ser831) might play a role in the induction of inflammatory but not neuropathic pain.

Authors+Show Affiliations

Neuroscience Research Institute, Peking University, 38 Xueyuan Road, Beijing 100083, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18289517

Citation

Lu, Yue, et al. "Role of Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) Receptor Subunit GluR1 in Spinal Dorsal Horn in Inflammatory Nociception and Neuropathic Nociception in Rat." Brain Research, vol. 1200, 2008, pp. 19-26.
Lu Y, Sun YN, Wu X, et al. Role of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit GluR1 in spinal dorsal horn in inflammatory nociception and neuropathic nociception in rat. Brain Res. 2008;1200:19-26.
Lu, Y., Sun, Y. N., Wu, X., Sun, Q., Liu, F. Y., Xing, G. G., & Wan, Y. (2008). Role of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit GluR1 in spinal dorsal horn in inflammatory nociception and neuropathic nociception in rat. Brain Research, 1200, 19-26. https://doi.org/10.1016/j.brainres.2008.01.012
Lu Y, et al. Role of Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) Receptor Subunit GluR1 in Spinal Dorsal Horn in Inflammatory Nociception and Neuropathic Nociception in Rat. Brain Res. 2008 Mar 20;1200:19-26. PubMed PMID: 18289517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor subunit GluR1 in spinal dorsal horn in inflammatory nociception and neuropathic nociception in rat. AU - Lu,Yue, AU - Sun,Yan-Ni, AU - Wu,Xi, AU - Sun,Qian, AU - Liu,Feng-Yu, AU - Xing,Guo-Gang, AU - Wan,You, Y1 - 2008/01/16/ PY - 2007/02/14/received PY - 2007/12/05/revised PY - 2008/01/06/accepted PY - 2008/2/22/pubmed PY - 2008/7/19/medline PY - 2008/2/22/entrez SP - 19 EP - 26 JF - Brain research JO - Brain Res VL - 1200 N2 - The present study aims to investigate changes of spinal cord AMPA receptor GluR1 and its phosphorylation in inflammatory and neuropathic pain. Complete Freund's adjuvant (CFA) injection into the hind paw produced inflammatory thermal hyperalgesia that was assessed by decreased response latency to radiant heat; spinal nerve ligation (SNL) was used to induce mechanical allodynia that was evaluated with von Frey hairs. By method of Western blot, expression of GluR1 (the main subunit of the AMPA receptor) and its phosphorylated forms at serine 845 (pGluR1-Ser845) and at serine 831 (pGluR1-Ser831) in the spinal dorsal horn was observed. It was found that the expression of pGluR1-Ser845 and pGluR1-Ser831 increased significantly at 1 h after CFA injection, reached peak at 4 h and returned to the normal control level at 24 h, while no significant change was detected in GluR1 itself. In contrast, neither GluR1 nor pGluR1 showed any significant change in rats following SNL. These results suggest that phosphorylated GluR1 (pGluR1-Ser845 and pGluR1-Ser831) might play a role in the induction of inflammatory but not neuropathic pain. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/18289517/Role_of_alpha_amino_3_hydroxy_5_methyl_4_isoxazolepropionate__AMPA__receptor_subunit_GluR1_in_spinal_dorsal_horn_in_inflammatory_nociception_and_neuropathic_nociception_in_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(08)00043-7 DB - PRIME DP - Unbound Medicine ER -