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Cetyltrimethylammonium bromide-modified spherical and cube-like gold nanoparticles as extrinsic Raman labels in surface-enhanced Raman spectroscopy based heterogeneous immunoassays.
Anal Chem. 2008 Mar 15; 80(6):2265-71.AC

Abstract

This paper reports on the characterization and preliminary comparison of gold nanoparticles of differing surface modification and shape when used as extrinsic Raman labels (ERLs) in high-sensitivity heterogeneous immunoassays based on surface enhanced Raman scattering (SERS). ERLs are gold nanoparticles coated with an adlayer of an intrinsically strong Raman scatterer, followed by a coating of a molecular recognition element (e.g., antibody). Three types of ERLs, all with a nominal size of approximately 30 nm, were fabricated by using spherical citrate-capped gold nanoparticles (sp-cit-Au NPs), spherical CTAB-capped gold nanoparticles (sp-CTAB-Au NPs), or cube-like CTAB-capped gold nanoparticles (cu-CTAB-Au NPs) as cores. The performance of these particles was assessed via a sandwich immunoassay for human IgG in phosphate buffered saline. The ERLs fabricated with sp-CTAB-Au NPs as cores proved to be more than 50 times more sensitive than those with sp-cit-Au NPs as cores; the same comparison showed that the ERLs with cu-CTAB-Au NPs as cores were close to 200 times more sensitive. Coupled with small differences in levels of nonspecific adsorption, these sensitivities translated to a limit of detection (LOD) of 94, 2.3, and 0.28 ng/mL, respectively, for the detection of human IgG in the case of sp-cit-Au NPs, sp-CTAB-Au NPs, and cu-CTAB-Au NPs. The LOD of the cu-CTAB-Au NPs is therefore approximately 340 times below that for the sp-cit-Au NPs. Potential applications of these labels to bioassays are briefly discussed.

Authors+Show Affiliations

Department of Chemistry, University of Utah, Salt Lake City, Utah 84112-0850, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18290676

Citation

Narayanan, Radha, et al. "Cetyltrimethylammonium Bromide-modified Spherical and Cube-like Gold Nanoparticles as Extrinsic Raman Labels in Surface-enhanced Raman Spectroscopy Based Heterogeneous Immunoassays." Analytical Chemistry, vol. 80, no. 6, 2008, pp. 2265-71.
Narayanan R, Lipert RJ, Porter MD. Cetyltrimethylammonium bromide-modified spherical and cube-like gold nanoparticles as extrinsic Raman labels in surface-enhanced Raman spectroscopy based heterogeneous immunoassays. Anal Chem. 2008;80(6):2265-71.
Narayanan, R., Lipert, R. J., & Porter, M. D. (2008). Cetyltrimethylammonium bromide-modified spherical and cube-like gold nanoparticles as extrinsic Raman labels in surface-enhanced Raman spectroscopy based heterogeneous immunoassays. Analytical Chemistry, 80(6), 2265-71. https://doi.org/10.1021/ac7026436
Narayanan R, Lipert RJ, Porter MD. Cetyltrimethylammonium Bromide-modified Spherical and Cube-like Gold Nanoparticles as Extrinsic Raman Labels in Surface-enhanced Raman Spectroscopy Based Heterogeneous Immunoassays. Anal Chem. 2008 Mar 15;80(6):2265-71. PubMed PMID: 18290676.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cetyltrimethylammonium bromide-modified spherical and cube-like gold nanoparticles as extrinsic Raman labels in surface-enhanced Raman spectroscopy based heterogeneous immunoassays. AU - Narayanan,Radha, AU - Lipert,Robert J, AU - Porter,Marc D, Y1 - 2008/02/22/ PY - 2008/2/23/pubmed PY - 2008/4/30/medline PY - 2008/2/23/entrez SP - 2265 EP - 71 JF - Analytical chemistry JO - Anal Chem VL - 80 IS - 6 N2 - This paper reports on the characterization and preliminary comparison of gold nanoparticles of differing surface modification and shape when used as extrinsic Raman labels (ERLs) in high-sensitivity heterogeneous immunoassays based on surface enhanced Raman scattering (SERS). ERLs are gold nanoparticles coated with an adlayer of an intrinsically strong Raman scatterer, followed by a coating of a molecular recognition element (e.g., antibody). Three types of ERLs, all with a nominal size of approximately 30 nm, were fabricated by using spherical citrate-capped gold nanoparticles (sp-cit-Au NPs), spherical CTAB-capped gold nanoparticles (sp-CTAB-Au NPs), or cube-like CTAB-capped gold nanoparticles (cu-CTAB-Au NPs) as cores. The performance of these particles was assessed via a sandwich immunoassay for human IgG in phosphate buffered saline. The ERLs fabricated with sp-CTAB-Au NPs as cores proved to be more than 50 times more sensitive than those with sp-cit-Au NPs as cores; the same comparison showed that the ERLs with cu-CTAB-Au NPs as cores were close to 200 times more sensitive. Coupled with small differences in levels of nonspecific adsorption, these sensitivities translated to a limit of detection (LOD) of 94, 2.3, and 0.28 ng/mL, respectively, for the detection of human IgG in the case of sp-cit-Au NPs, sp-CTAB-Au NPs, and cu-CTAB-Au NPs. The LOD of the cu-CTAB-Au NPs is therefore approximately 340 times below that for the sp-cit-Au NPs. Potential applications of these labels to bioassays are briefly discussed. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/18290676/Cetyltrimethylammonium_bromide_modified_spherical_and_cube_like_gold_nanoparticles_as_extrinsic_Raman_labels_in_surface_enhanced_Raman_spectroscopy_based_heterogeneous_immunoassays_ L2 - https://doi.org/10.1021/ac7026436 DB - PRIME DP - Unbound Medicine ER -