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Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction.
Anal Chim Acta. 2008 Mar 10; 610(2):274-81.AC

Abstract

Coconut (Cocos nucifera L.) water, which contains many uncharacterized phytohormones is extensively used as a growth promoting supplement in plant tissue culture. In this paper, a high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of various classes phytohormones, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N(6)-benzyladenine (BA), alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) in young coconut water (CW). The analysis was carried out using a reverse-phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid, pH adjusted to 3.2 with triethylamine (TEA)) modified by methanol, and solute detection made at 265 nm wavelength. The method was validated for specificity, quantification, accuracy and precision. After preconcentration of putative endogenous phytohormones in CW using C(18) solid-phase extraction (SPE) cartridges, the HPLC method was able to screen for putative endogenous phytohormones present in CW. Finally, the identities of the putative phytohormones present in CW were further confirmed using independent liquid chromatography-tandem mass spectrometry (LC-MS/MS) equipped with an electrospray ionization (ESI) interface.

Authors+Show Affiliations

Natural Sciences and Science Education Academic Group, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18291140

Citation

Ma, Zhen, et al. "Simultaneous Analysis of Different Classes of Phytohormones in Coconut (Cocos Nucifera L.) Water Using High-performance Liquid Chromatography and Liquid Chromatography-tandem Mass Spectrometry After Solid-phase Extraction." Analytica Chimica Acta, vol. 610, no. 2, 2008, pp. 274-81.
Ma Z, Ge L, Lee AS, et al. Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction. Anal Chim Acta. 2008;610(2):274-81.
Ma, Z., Ge, L., Lee, A. S., Yong, J. W., Tan, S. N., & Ong, E. S. (2008). Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction. Analytica Chimica Acta, 610(2), 274-81. https://doi.org/10.1016/j.aca.2008.01.045
Ma Z, et al. Simultaneous Analysis of Different Classes of Phytohormones in Coconut (Cocos Nucifera L.) Water Using High-performance Liquid Chromatography and Liquid Chromatography-tandem Mass Spectrometry After Solid-phase Extraction. Anal Chim Acta. 2008 Mar 10;610(2):274-81. PubMed PMID: 18291140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction. AU - Ma,Zhen, AU - Ge,Liya, AU - Lee,Anna S Y, AU - Yong,Jean Wan Hong, AU - Tan,Swee Ngin, AU - Ong,Eng Shi, Y1 - 2008/01/25/ PY - 2007/11/16/received PY - 2008/01/17/revised PY - 2008/01/17/accepted PY - 2008/2/23/pubmed PY - 2008/8/9/medline PY - 2008/2/23/entrez SP - 274 EP - 81 JF - Analytica chimica acta JO - Anal Chim Acta VL - 610 IS - 2 N2 - Coconut (Cocos nucifera L.) water, which contains many uncharacterized phytohormones is extensively used as a growth promoting supplement in plant tissue culture. In this paper, a high-performance liquid chromatography (HPLC) method was developed for the simultaneous determination of various classes phytohormones, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N(6)-benzyladenine (BA), alpha-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) in young coconut water (CW). The analysis was carried out using a reverse-phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid, pH adjusted to 3.2 with triethylamine (TEA)) modified by methanol, and solute detection made at 265 nm wavelength. The method was validated for specificity, quantification, accuracy and precision. After preconcentration of putative endogenous phytohormones in CW using C(18) solid-phase extraction (SPE) cartridges, the HPLC method was able to screen for putative endogenous phytohormones present in CW. Finally, the identities of the putative phytohormones present in CW were further confirmed using independent liquid chromatography-tandem mass spectrometry (LC-MS/MS) equipped with an electrospray ionization (ESI) interface. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/18291140/Simultaneous_analysis_of_different_classes_of_phytohormones_in_coconut__Cocos_nucifera_L___water_using_high_performance_liquid_chromatography_and_liquid_chromatography_tandem_mass_spectrometry_after_solid_phase_extraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(08)00138-4 DB - PRIME DP - Unbound Medicine ER -