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Glutamate-stimulated release of norepinephrine in hippocampal slices of animal models of attention-deficit/hyperactivity disorder (spontaneously hypertensive rat) and depression/anxiety-like behaviours (Wistar-Kyoto rat).
Brain Res. 2008 Mar 20; 1200:107-15.BR

Abstract

Norepinephrine is known to play an integral role in different aspects of behaviour, such as attention and arousal. It has also been implicated in the neurobiology of attention-deficit/hyperactivity disorder (ADHD). The present study was undertaken to determine the differential effects of glutamate on norepinephrine release in hippocampal slices of several rat strains. Two of the strains used in this study model behavioural disorders i.e. spontaneously hypertensive rats (SHR) mimic the behavioural characteristics of ADHD and Wistar-Kyoto (WKY) rats have been used to model depression/anxiety-like behaviours. To achieve the aims of this study, an in vitro superfusion technique was used to determine glutamate-stimulated release of radioactively labelled norepinephrine in hippocampal slices. The results show (1) SHR and Wistar rats released significantly more [(3)H]norepinephrine in response to a 1-min pulse of glutamate (1 mM) than WKY, Sprague-Dawley and Long-Evans rats. (2) Glutamate-stimulated release of [(3)H]norepinephrine was reduced by the AMPA receptor antagonist, CNQX (1 muM), suggesting that AMPA receptors are involved. (3) Exposure of hippocampal slices to a second and third 1-min pulse of glutamate revealed significant decreases in the peaks of [(3)H]norepinephrine release suggesting internalization of AMPA receptors. The rate of AMPA receptor internalization was slower in SHR than in WKY. (4) The NMDA receptor antagonist, MK-801 (10 microM) increased glutamate-stimulated release of [(3)H]norepinephrine in SHR hippocampus. This effect was blocked by CNQX, suggesting that AMPA receptors were required for the NMDA effect and that there was an NMDA component of AMPA receptor internalization in SHR hippocampus which was not evident in WKY. The present findings reveal a novel NMDA component that influences AMPA receptor-mediated regulation of norepinephrine release in SHR hippocampus.

Authors+Show Affiliations

Neuroscience Laboratory, Division of Physiology, Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory, 7925 South Africa.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18295191

Citation

Howells, Fleur Margaret, and Vivienne Ann Russell. "Glutamate-stimulated Release of Norepinephrine in Hippocampal Slices of Animal Models of Attention-deficit/hyperactivity Disorder (spontaneously Hypertensive Rat) and Depression/anxiety-like Behaviours (Wistar-Kyoto Rat)." Brain Research, vol. 1200, 2008, pp. 107-15.
Howells FM, Russell VA. Glutamate-stimulated release of norepinephrine in hippocampal slices of animal models of attention-deficit/hyperactivity disorder (spontaneously hypertensive rat) and depression/anxiety-like behaviours (Wistar-Kyoto rat). Brain Res. 2008;1200:107-15.
Howells, F. M., & Russell, V. A. (2008). Glutamate-stimulated release of norepinephrine in hippocampal slices of animal models of attention-deficit/hyperactivity disorder (spontaneously hypertensive rat) and depression/anxiety-like behaviours (Wistar-Kyoto rat). Brain Research, 1200, 107-15. https://doi.org/10.1016/j.brainres.2008.01.033
Howells FM, Russell VA. Glutamate-stimulated Release of Norepinephrine in Hippocampal Slices of Animal Models of Attention-deficit/hyperactivity Disorder (spontaneously Hypertensive Rat) and Depression/anxiety-like Behaviours (Wistar-Kyoto Rat). Brain Res. 2008 Mar 20;1200:107-15. PubMed PMID: 18295191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutamate-stimulated release of norepinephrine in hippocampal slices of animal models of attention-deficit/hyperactivity disorder (spontaneously hypertensive rat) and depression/anxiety-like behaviours (Wistar-Kyoto rat). AU - Howells,Fleur Margaret, AU - Russell,Vivienne Ann, Y1 - 2008/01/26/ PY - 2007/10/12/received PY - 2008/01/02/revised PY - 2008/01/03/accepted PY - 2008/2/26/pubmed PY - 2008/7/19/medline PY - 2008/2/26/entrez SP - 107 EP - 15 JF - Brain research JO - Brain Res VL - 1200 N2 - Norepinephrine is known to play an integral role in different aspects of behaviour, such as attention and arousal. It has also been implicated in the neurobiology of attention-deficit/hyperactivity disorder (ADHD). The present study was undertaken to determine the differential effects of glutamate on norepinephrine release in hippocampal slices of several rat strains. Two of the strains used in this study model behavioural disorders i.e. spontaneously hypertensive rats (SHR) mimic the behavioural characteristics of ADHD and Wistar-Kyoto (WKY) rats have been used to model depression/anxiety-like behaviours. To achieve the aims of this study, an in vitro superfusion technique was used to determine glutamate-stimulated release of radioactively labelled norepinephrine in hippocampal slices. The results show (1) SHR and Wistar rats released significantly more [(3)H]norepinephrine in response to a 1-min pulse of glutamate (1 mM) than WKY, Sprague-Dawley and Long-Evans rats. (2) Glutamate-stimulated release of [(3)H]norepinephrine was reduced by the AMPA receptor antagonist, CNQX (1 muM), suggesting that AMPA receptors are involved. (3) Exposure of hippocampal slices to a second and third 1-min pulse of glutamate revealed significant decreases in the peaks of [(3)H]norepinephrine release suggesting internalization of AMPA receptors. The rate of AMPA receptor internalization was slower in SHR than in WKY. (4) The NMDA receptor antagonist, MK-801 (10 microM) increased glutamate-stimulated release of [(3)H]norepinephrine in SHR hippocampus. This effect was blocked by CNQX, suggesting that AMPA receptors were required for the NMDA effect and that there was an NMDA component of AMPA receptor internalization in SHR hippocampus which was not evident in WKY. The present findings reveal a novel NMDA component that influences AMPA receptor-mediated regulation of norepinephrine release in SHR hippocampus. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/18295191/Glutamate_stimulated_release_of_norepinephrine_in_hippocampal_slices_of_animal_models_of_attention_deficit/hyperactivity_disorder__spontaneously_hypertensive_rat__and_depression/anxiety_like_behaviours__Wistar_Kyoto_rat__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(08)00030-9 DB - PRIME DP - Unbound Medicine ER -