Tags

Type your tag names separated by a space and hit enter

Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages.
Eur J Pharmacol 2008; 584(1):175-84EJ

Abstract

In this study, the anti-inflammatory effects of flavonoids isolated from the roots of Glycyrrhiza uralensis (Leguminosae), namely, isoliquiritin (the glycoside of isoliquirigenin) and isoliquiritigenin (the aglycone of isoliquiritin) were evaluated on lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Isoliquiritigenin (ILG) more potently inhibited LPS-induced nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production than isoliquiritin (ILT). Consistent with these findings, ILG reduced the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner, as determined by Western blotting and RT-PCR, respectively. In addition, the release of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), and the mRNA expression levels of these cytokines were reduced by ILG in a dose-dependent manner. Moreover, ILG attenuated the LPS-induced DNA binding activity and the transcription activity of nuclear factor-kappa B (NF-kappaB), and this was associated with a decrease in inhibitory kappa B-alpha (IkappaB-alpha) phosphorylation and in the subsequent blocking of p65 and p50 protein translocations to the nucleus. Furthermore, ILG suppressed the phosphorylations of IkappaB kinase (IKK), ERK1/2, and p38, whereas the phosphorylation of JNK1/2 was unaffected. These results suggest that the anti-inflammatory properties of ILG are caused by iNOS, COX-2, TNF-alpha, and IL-6 down-regulation due to NF-kappaB inhibition via the suppression of IKK, ERK1/2 and p38 phosphorylation in RAW 264.7 cells.

Authors+Show Affiliations

Department of Pharmaceutical Biochemistry, Kyung-Hee University, Dongdaemun-ku, Hoegi-Dong, Seoul 130-701, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18295200

Citation

Kim, Ji-Yeon, et al. "Isoliquiritigenin Isolated From the Roots of Glycyrrhiza Uralensis Inhibits LPS-induced iNOS and COX-2 Expression Via the Attenuation of NF-kappaB in RAW 264.7 Macrophages." European Journal of Pharmacology, vol. 584, no. 1, 2008, pp. 175-84.
Kim JY, Park SJ, Yun KJ, et al. Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages. Eur J Pharmacol. 2008;584(1):175-84.
Kim, J. Y., Park, S. J., Yun, K. J., Cho, Y. W., Park, H. J., & Lee, K. T. (2008). Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages. European Journal of Pharmacology, 584(1), pp. 175-84. doi:10.1016/j.ejphar.2008.01.032.
Kim JY, et al. Isoliquiritigenin Isolated From the Roots of Glycyrrhiza Uralensis Inhibits LPS-induced iNOS and COX-2 Expression Via the Attenuation of NF-kappaB in RAW 264.7 Macrophages. Eur J Pharmacol. 2008 Apr 14;584(1):175-84. PubMed PMID: 18295200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages. AU - Kim,Ji-Yeon, AU - Park,Seung Jae, AU - Yun,Kyung-Jin, AU - Cho,Young-Wuk, AU - Park,Hee-Juhn, AU - Lee,Kyung-Tae, Y1 - 2008/02/05/ PY - 2007/09/03/received PY - 2008/01/03/revised PY - 2008/01/22/accepted PY - 2008/2/26/pubmed PY - 2008/7/11/medline PY - 2008/2/26/entrez SP - 175 EP - 84 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 584 IS - 1 N2 - In this study, the anti-inflammatory effects of flavonoids isolated from the roots of Glycyrrhiza uralensis (Leguminosae), namely, isoliquiritin (the glycoside of isoliquirigenin) and isoliquiritigenin (the aglycone of isoliquiritin) were evaluated on lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. Isoliquiritigenin (ILG) more potently inhibited LPS-induced nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production than isoliquiritin (ILT). Consistent with these findings, ILG reduced the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner, as determined by Western blotting and RT-PCR, respectively. In addition, the release of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), and the mRNA expression levels of these cytokines were reduced by ILG in a dose-dependent manner. Moreover, ILG attenuated the LPS-induced DNA binding activity and the transcription activity of nuclear factor-kappa B (NF-kappaB), and this was associated with a decrease in inhibitory kappa B-alpha (IkappaB-alpha) phosphorylation and in the subsequent blocking of p65 and p50 protein translocations to the nucleus. Furthermore, ILG suppressed the phosphorylations of IkappaB kinase (IKK), ERK1/2, and p38, whereas the phosphorylation of JNK1/2 was unaffected. These results suggest that the anti-inflammatory properties of ILG are caused by iNOS, COX-2, TNF-alpha, and IL-6 down-regulation due to NF-kappaB inhibition via the suppression of IKK, ERK1/2 and p38 phosphorylation in RAW 264.7 cells. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18295200/Isoliquiritigenin_isolated_from_the_roots_of_Glycyrrhiza_uralensis_inhibits_LPS_induced_iNOS_and_COX_2_expression_via_the_attenuation_of_NF_kappaB_in_RAW_264_7_macrophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(08)00104-0 DB - PRIME DP - Unbound Medicine ER -