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Rapid preparation of procyanidins B2 and C1 from Granny Smith apples by using low pressure column chromatography and identification of their oligomeric procyanidins.
J Agric Food Chem. 2008 Mar 26; 56(6):2096-101.JA

Abstract

Research in the field of procyanidins is always hindered by the lack of procyanidin standards, and the preparation of procyanidins, especially in large scale, remains difficult and time-consuming. Commercial sources of procyanidin standards are scarce. In this study, a rapid preparation method of procyanidins by using low-pressure column chromatography was developed. Procyanidins in Granny Smith apples were extracted with boiled water and purified on an ADS-17 macroporous resin column to obtain a Granny Smith apple procyanidin extract (GSE). GSE was fractionated according to its degree of polymerization on a Toyopearl TSK HW-40s column. Procyanidins B2 (epicatechin-(4beta-8)-epicatechin) and C1 (epicatechin-(4beta-8)-epicatechin-(4beta-8)-epicatechin) were prepared without HPLC separation. Oligomeric procyanidins from Granny Smith apples were also identified by liquid chromatography-electrospray ionization-mass spectrometry.

Authors+Show Affiliations

College of Food Science and Technology, Huazhong Agricultural University, Wuhan, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18298060

Citation

Xiao, Jun-Song, et al. "Rapid Preparation of Procyanidins B2 and C1 From Granny Smith Apples By Using Low Pressure Column Chromatography and Identification of Their Oligomeric Procyanidins." Journal of Agricultural and Food Chemistry, vol. 56, no. 6, 2008, pp. 2096-101.
Xiao JS, Liu L, Wu H, et al. Rapid preparation of procyanidins B2 and C1 from Granny Smith apples by using low pressure column chromatography and identification of their oligomeric procyanidins. J Agric Food Chem. 2008;56(6):2096-101.
Xiao, J. S., Liu, L., Wu, H., Xie, B. J., Yang, E. N., & Sun, Z. D. (2008). Rapid preparation of procyanidins B2 and C1 from Granny Smith apples by using low pressure column chromatography and identification of their oligomeric procyanidins. Journal of Agricultural and Food Chemistry, 56(6), 2096-101. https://doi.org/10.1021/jf072679x
Xiao JS, et al. Rapid Preparation of Procyanidins B2 and C1 From Granny Smith Apples By Using Low Pressure Column Chromatography and Identification of Their Oligomeric Procyanidins. J Agric Food Chem. 2008 Mar 26;56(6):2096-101. PubMed PMID: 18298060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid preparation of procyanidins B2 and C1 from Granny Smith apples by using low pressure column chromatography and identification of their oligomeric procyanidins. AU - Xiao,Jun-Song, AU - Liu,Liang, AU - Wu,Hua, AU - Xie,Bi-Jun, AU - Yang,Er-Ning, AU - Sun,Zhi-Da, Y1 - 2008/02/26/ PY - 2008/2/27/pubmed PY - 2008/6/12/medline PY - 2008/2/27/entrez SP - 2096 EP - 101 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 56 IS - 6 N2 - Research in the field of procyanidins is always hindered by the lack of procyanidin standards, and the preparation of procyanidins, especially in large scale, remains difficult and time-consuming. Commercial sources of procyanidin standards are scarce. In this study, a rapid preparation method of procyanidins by using low-pressure column chromatography was developed. Procyanidins in Granny Smith apples were extracted with boiled water and purified on an ADS-17 macroporous resin column to obtain a Granny Smith apple procyanidin extract (GSE). GSE was fractionated according to its degree of polymerization on a Toyopearl TSK HW-40s column. Procyanidins B2 (epicatechin-(4beta-8)-epicatechin) and C1 (epicatechin-(4beta-8)-epicatechin-(4beta-8)-epicatechin) were prepared without HPLC separation. Oligomeric procyanidins from Granny Smith apples were also identified by liquid chromatography-electrospray ionization-mass spectrometry. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/18298060/Rapid_preparation_of_procyanidins_B2_and_C1_from_Granny_Smith_apples_by_using_low_pressure_column_chromatography_and_identification_of_their_oligomeric_procyanidins_ L2 - https://dx.doi.org/10.1021/jf072679x DB - PRIME DP - Unbound Medicine ER -