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The role of macrophages in the periodontal regeneration using Emdogain gel.
J Periodontal Res 2008; 43(2):143-55JP

Abstract

BACKGROUND AND OBJECTIVE

Emdogain gel is clinically used as a periodontal regenerative material. However, the mechanism of the regeneration has not been completely elucidated. Although many studies have focused on the regenerative effect of Emdogain on connective tissue attachment and alveolar bone, the role of macrophages and the expression of growth factors remains unclear in the regeneration stimulated by Emdogain gel in vivo. The aim of this study was to investigate the effect of Emdogain gel on the expression of cytokines and growth factors by macrophages in vivo using a newly devised rat experimental periodontitis model.

MATERIAL AND METHODS

Rat experimental periodontitis was induced by elevating a full-thickness gingival flap and ligating silk threads around the first molars of the mandible. At 14 d after inducing experimental periodontitis, Emdogain gel or propylene glycol alginate was applied to the furcation area. The rats were killed 7 and 14 d after treatment with propylene glycol alginate or Emdogain gel. The expression of cytokines and growth factors, and the regeneration of periodontal tissue, were examined by histochemical and immunohistochemical methods.

RESULTS

Fourteen days after the induction of periodontitis, the resorption of alveolar bone at furcation was observed and cytokines such as interleukin-1beta, transforming growth factor-beta1, receptor activator of nuclear factor-kappaB ligand, receptor activator of nuclear factor-kappaB and osteoprotegerin were found. In the Emdogain-treatment group, the formation of new acellular cementum and, more remarkably, recovery of the bone, were observed. The new bone formation ratio in the Emdogain treatment group was significantly higher than that of the propylene glycol alginate treatment group. Although the expression of cytokines such as interleukin-1beta, transforming growth factor-beta1, receptor activator of nuclear factor-kappaB ligand and receptor activator of nuclear factor-kappaB was very low, bone morphogenetic protein-2- and bone morphogenetic protein-4-expressing macrophages were observed close to the root, and bone morphogenetic protein-4-expressing macrophages were mainly observed close to the bone surface at the furcation in the Emdogain-treatment group.

CONCLUSION

These results suggest that wound-healing macrophages may express bone morphogenetic protein and play an important role in the regeneration of periodontal tissue at the furcation following the application of Emdogain gel.

Authors+Show Affiliations

Section of Periodontology, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18302615

Citation

Fujishiro, N, et al. "The Role of Macrophages in the Periodontal Regeneration Using Emdogain Gel." Journal of Periodontal Research, vol. 43, no. 2, 2008, pp. 143-55.
Fujishiro N, Anan H, Hamachi T, et al. The role of macrophages in the periodontal regeneration using Emdogain gel. J Periodont Res. 2008;43(2):143-55.
Fujishiro, N., Anan, H., Hamachi, T., & Maeda, K. (2008). The role of macrophages in the periodontal regeneration using Emdogain gel. Journal of Periodontal Research, 43(2), pp. 143-55. doi:10.1111/j.1600-0765.2007.01004.x.
Fujishiro N, et al. The Role of Macrophages in the Periodontal Regeneration Using Emdogain Gel. J Periodont Res. 2008;43(2):143-55. PubMed PMID: 18302615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of macrophages in the periodontal regeneration using Emdogain gel. AU - Fujishiro,N, AU - Anan,H, AU - Hamachi,T, AU - Maeda,K, PY - 2008/2/28/pubmed PY - 2008/3/19/medline PY - 2008/2/28/entrez SP - 143 EP - 55 JF - Journal of periodontal research JO - J. Periodont. Res. VL - 43 IS - 2 N2 - BACKGROUND AND OBJECTIVE: Emdogain gel is clinically used as a periodontal regenerative material. However, the mechanism of the regeneration has not been completely elucidated. Although many studies have focused on the regenerative effect of Emdogain on connective tissue attachment and alveolar bone, the role of macrophages and the expression of growth factors remains unclear in the regeneration stimulated by Emdogain gel in vivo. The aim of this study was to investigate the effect of Emdogain gel on the expression of cytokines and growth factors by macrophages in vivo using a newly devised rat experimental periodontitis model. MATERIAL AND METHODS: Rat experimental periodontitis was induced by elevating a full-thickness gingival flap and ligating silk threads around the first molars of the mandible. At 14 d after inducing experimental periodontitis, Emdogain gel or propylene glycol alginate was applied to the furcation area. The rats were killed 7 and 14 d after treatment with propylene glycol alginate or Emdogain gel. The expression of cytokines and growth factors, and the regeneration of periodontal tissue, were examined by histochemical and immunohistochemical methods. RESULTS: Fourteen days after the induction of periodontitis, the resorption of alveolar bone at furcation was observed and cytokines such as interleukin-1beta, transforming growth factor-beta1, receptor activator of nuclear factor-kappaB ligand, receptor activator of nuclear factor-kappaB and osteoprotegerin were found. In the Emdogain-treatment group, the formation of new acellular cementum and, more remarkably, recovery of the bone, were observed. The new bone formation ratio in the Emdogain treatment group was significantly higher than that of the propylene glycol alginate treatment group. Although the expression of cytokines such as interleukin-1beta, transforming growth factor-beta1, receptor activator of nuclear factor-kappaB ligand and receptor activator of nuclear factor-kappaB was very low, bone morphogenetic protein-2- and bone morphogenetic protein-4-expressing macrophages were observed close to the root, and bone morphogenetic protein-4-expressing macrophages were mainly observed close to the bone surface at the furcation in the Emdogain-treatment group. CONCLUSION: These results suggest that wound-healing macrophages may express bone morphogenetic protein and play an important role in the regeneration of periodontal tissue at the furcation following the application of Emdogain gel. SN - 1600-0765 UR - https://www.unboundmedicine.com/medline/citation/18302615/The_role_of_macrophages_in_the_periodontal_regeneration_using_Emdogain_gel_ L2 - https://doi.org/10.1111/j.1600-0765.2007.01004.x DB - PRIME DP - Unbound Medicine ER -